Cytokine (interleukin‐1beta) and MMP levels in gingival crevicular fluid after use of platelet‐rich fibrin or connective tissue graft in the treatment of localized gingival recessions

Abstract

Root coverage with CAF+PRF has a significant effect on increasing gingival crevicular fluid TIMP-1 levels and suppressing gingival crevicular fluid MMP-8 and IL-β levels at 10 d. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-1 and IL-1β did not seem to be affected by the technique at later phases of wound healing.

Platelet-Rich Fibrin in Coverage of Gingival Recession: A Systematic Review and Meta-Analysis

“…These molecules include metalloproteases (MMPs) and their tissue inhibitors (TIMPs), which are responsible for remodeling the tissue collagen matrix. 15,42 MMPs make up a family of proteolytic enzymes that are active in pathological and physiological processes involved in collagen remodeling and tissue repair. 40 These proteins are divided into two groups and are regulated by TIMPs, which are endogenous proteins.…”

“…13,43 In homeostasis, MMPs and TIMPs are in equilibrium, but trauma such as that which is caused by surgery can trigger substantial metabolic and immunological reactions that disrupt this balance and affect the tissue repair process. 15,44 Despite the role of these molecules in wound healing, only one of the articles included in this review addressed the molecular aspects of the healing process. The authors evaluated changes in MMP and TIMP levels over time in the control group (SCTG) and the experimental group (PRF).…”

“…7,8 Recent studies have recommended the use of PRF membranes for the treatment of gingival recession as an alternative to SCTGs. [9][10][11][12][13][14][15][16][17] However, because it is a new type of biomaterial that has not been tested in many prospective follow-up studies, issues such as root coverage predictability, effects on healing, and the molecular mechanisms of action involved in these processes have yet to be fully understood. 12 These factors support this systematic review of the literature and meta-analysis, which together seek to objectively present the most recent findings on the efficacy of PRF as an alternative to the gold standard in the coverage of Miller class I and II gingival recessions.…”

Effects of leukocyte‐ and platelet‐rich fibrin associated or not with bovine bone graft on the healing of bone defects in rats with osteoporosis induced by ovariectomy

“…Leukocyte‐ and platelet‐rich fibrin (L‐PRF) has been investigated as a promising autologous source of cytokines and growth factors that favor tissue regeneration (Choukroun et al, ; Choukroun et al, ; Dohan Ehrenfest et al, ; He, Lin, Hu, Zhang, and Wu, ; Kang et al, ; Simonpieri et al, ; Ghanaati et al, ; Zhao et al, ). In clinical practice, L‐PRF has been applied in different areas that seek excellence for the healing processes of soft and hard tissues (Braccini and Dohan, ; Chang et al, ; Eren, Tervahartiala, Sorsa, and Atilla, ; Eshghpour et al, ; Canellas et al, ; Somani and Rai, ). Initially described by Choukroun, Adda, Schoeffler, and Vervelle () in France, L‐PRF is considered a second‐generation platelet concentrate and is characterized as a true autologous healing matrix (Choukroun et al, ; Dohan et al, ).…”

“…Some studies have demonstrated that L‐PRF compressed clot alone produced favorable clinical outcomes in the treatment of infrabony periodontal defects (Chang et al, ; Zhao et al, ; Sharma and Pradeep, ; Ajwani et al, ; Chandradas et al, ), protected open wounds in the oral cavity (Asaka et al, ), sped up the healing of hard and soft tissues (Marenzi et al, ; Simon et al, ; Boora et al, ; Tunali et al, ; Eren et al, ; Kotsakis et al, ), reduced the rates of alveolitis after tooth extractions (Eshghpour et al, ; Kumar et al, ), and improved BV in preprosthetic surgeries and the osseointegration of dental implants (Oncu and Erbeyoglu, ; Castro et al, ; Cortese et al, ; Kanayama et al, ; Aoki et al, ; Oncu and Alaaddinoglu, ; Oncu et al, ; Boora et al, ; Simon et al, ).…”