Cytokine-Producing Function of Peripheral Blood Cells in Colorectal Cancer Patients

Sosnina, A. V.; Autenshlyus, A. I.; Velikaya, N. V.; Вараксин, Н. А.; Rukhavishnikov, M. Yu.; Михайлова, Е. С.; Morozov, D. V.; Фурсов, С. А.; Agarina, N. V.

doi:10.15789/1563-0625-2011-2-3-197-204

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…The cytokine-producing activity of a tumour, its microenvironment profile and cytokine-producing potential were assessed using the CYTOKINE-STIMUL-BEST standardised as-say kit (Vector-Best Inc., Novosibirsk Region). Tumour biopsy samples (8 mm 3 ) obtained by trepanobiopsy 9 , 12 were placed into two glass vials containing 1 mL of a liquid each; one vial contained only the DMEM/F12 medium (analysis of spontaneous production of cytokines), while the second vial contained a mixture of polyclonal activators (PAs; consisting of 4 µg/mL phytohaemagglutinin, 4 µg/mL concanavalin A and 2 µg/mL lipopolysaccharide) in an equivalent amount of the medium (analysis of PA-stimulated cytokine production). The incubation of tumour biopsy samples lasted for 72 h. To obtain a culture supernatant, each tumour biopsy sample was taken out of the vial, and the remaining cells were pelleted by centrifugation at 900 g for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Cytokine production in mammary adenocarcinoma and its microenvironmental cells in patients with or without metastases in regional lymph nodes

Autenshlyus

Архипов

Михайлова

et al. 2017

Int J Immunopathol Pharmacol

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In recent years, the concept of formation of a sufficiently autonomous cytokine network in a malignant tumour has emerged. In this regard, the data on the role of this network and its signalling pathways in the process of metastasis are an interesting topic. The aim of this study was to evaluate the in vitro cytokine-producing potential of mammary adenocarcinoma (MAC; and cells of its microenvironment) from patients with or without metastases in regional lymph nodes (LNs). By enzyme-linked immunosorbent assays of culture supernatants, we studied the cytokine production by biopsy samples of MAC: spontaneous and stimulated by polyclonal activators (PAs: phytohaemagglutinin, concanavalin A and lipopolysaccharide). The levels of spontaneous production of interleukin (IL)-10 and granulocyte colony-stimulating factor (G-CSF) and the amounts of IL-2, IL-10, G-CSF and monocyte chemoattractant protein-1 (MCP-1) produced during stimulation by PAs, as well as the index of stimulation by polyclonal activators (ISPA) for IL-2 production, were lower for MAC with LN metastasis than for MAC without LN metastasis. The levels of spontaneous production of IL-2 and interferon (IFN)-γ and the ISPA for granulocyte–macrophage colony-stimulating factor (GM-CSF) production were higher for MAC with LN metastasis. There were only three pairwise correlations between the produced cytokines that were specific to MAC with LN metastasis: IL-2 and IFN-γ, IL-6 and GM-CSF, and IL-8 and GM-CSF. There were 10 pairwise correlations between the produced cytokines that were specific to nonmetastasising MAC: IL-6 and IL-10, IL-6 and MCP-1, IL-8 and IL-10, IL-8 and MCP-1, IL-10 and G-CSF, IL-10 and MCP-1, IFN-γ and MCP-1, MCP-1 and G-CSF, G-CSF and IL-1Ra, and GM-CSF and tumour necrosis factor (TNF)-α. Our data indicate that metastatic tumours show desynchronisation of many pathways of induction and synthesis of cytokines that are characteristic of nonmetastatic tumours.

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Section: Methodsmentioning

confidence: 99%

Cytokine production in mammary adenocarcinoma and its microenvironmental cells in patients with or without metastases in regional lymph nodes

Autenshlyus

Архипов

Михайлова

et al. 2017

Int J Immunopathol Pharmacol

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“…[1][2][3] A growing tumor and cells in its microenvironment can produce a variety of cytokines, some of which inhibit its growth and metastasis, while others ensure tumor progression. 4,5 Correlations have been found among increased concentrations of certain angiogenic Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors cytokines, the degree of tumor vascularization, proliferative activity of tumor cells, and the depth of tumor invasion. 3,5,6 The data obtained led to the following concept: in the course of tumor progression, a sufficiently autonomous cytokine network 4 can be formed in the tumor, and this network is one of the multicomponent regulators of malignant tumor growth and metastasis.…”

Section: Introductionmentioning

confidence: 99%

Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors

Autenshlyus

Архипов

Кунц

et al. 2017

Int J Immunopathol Pharmacol

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Interrelations between cytokines, produced by invasive ductal carcinoma (IDC) and fibroadenoma (FA) of the breast, and angiogenic growth factor VEGF-A, expressed in IDC and FA, were investigated. The analysis of the cytokine profiles of IDC and FA was performed by cultivation of tumor biopsy specimens in vitro. Testing of the cytokine-producing reserve of the tumors for production of VEGF-A was conducted by culturing samples of IDC and FA in a medium containing polyclonal activator (a complex of phytohemagglutinin, concanavalin A, and lipopolysaccharide). Levels of cytokines and growth factors (IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α, IFN-γ, G-CSF, GM-CSF, VEGF-A) and MCP-1 (monocyte chemoattractant protein-1) in tumor supernatants were determined by an ELISA. Expression of VEGF-A was analyzed in tumor biopsy specimens by immunohistochemical analysis. In the IDC supernatants, the concentrations of IL-17, IL-18, and IFN-γ were higher and the concentrations of IL-10 and MCP-1 were lower in comparison with the FA supernatants. We observed negative correlations between the macrophage infiltration and VEGF-A concentration in the IDC supernatants (r = −0.508; P = 0.011) and between VEGF-A expression and the IDC vascularization degree (r = −0.423, P = 0.039). Spontaneous expression of VEGF-A in samples of IDC significantly exceeded the VEGF-A expression in FA. There was no difference between IDC and FA in VEGF-A expression after treatment with the polyclonal activators. Our results indicate that greater malignancy may have a paradoxical effect that is controlled by cytokines and characterized by weakening of tumor angiogenesis during overproduction of VEGF-A. These findings point to complex mechanisms of positive and negative regulation of tumor angiogenesis by cytokines that are produced by the tumor and by cells in its microenvironment, whose cytokine profiles may change at different stages of tumor progression.

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The effects of tumor-derived supernatants (TDS) on cancer cell progression: A review and update on carcinogenesis and immunotherapy

Ahmadpour,

Habibi,

Ghazi

et al. 2024

Cancer Treatment and Research Communications

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Cytokine-Producing Function of Peripheral Blood Cells in Colorectal Cancer Patients

Cited by 5 publications

References 12 publications

Cytokine production in mammary adenocarcinoma and its microenvironmental cells in patients with or without metastases in regional lymph nodes

Cytokine production in mammary adenocarcinoma and its microenvironmental cells in patients with or without metastases in regional lymph nodes

Cytokine profiles of tumor supernatants in invasive ductal cancer and fibroadenoma of the breast and its relationship with VEGF-A expression in the tumors

The effects of tumor-derived supernatants (TDS) on cancer cell progression: A review and update on carcinogenesis and immunotherapy

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