Cytokine profile of iron-laden macrophages: Implications for cellular magnetic resonance imaging

Siglienti, Ines; Bendszus, Martin; Kleinschnitz, Christoph; Stoll, Guido

doi:10.1016/j.jneuroim.2005.11.011

Cited by 85 publications

(58 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, more detailed assessment of the immune response associated with these particles, such as a cytokine profile after exposure to the particles, could be interesting for the elucidation of their biological effects since they are immune-responding to foreign body. [44][45][46][47] However, this work confirmed the biocompatibility of BFO-NP and their utility as nanoprobes for biological -in particular diagnostics -applications.…”

Section: Discussionsupporting

confidence: 64%

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Staedler

Passemard

Magouroux

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

Bismuth Ferrite (BFO) nanoparticles (BFO-NP) display interesting optical (nonlinear response) and magnetic properties which make them amenable for bio-oriented diagnostic applications as intra-and extra membrane contrast agents. Due to the relatively recent availability of this material in well dispersed nanometric form, its biocompatibility was not known to date. In this study, we present a thorough assessment of the effects of in vitro exposure of human adenocarcinoma (A549), lung squamous carcinoma (NCI-H520), and acute monocytic leukemia (THP-1) cell lines to uncoated and poly(ethylene glycol)-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility. Our results support the attractiveness of the functional-BFO towards biomedical applications focused on advanced diagnostic imaging.

show abstract

Section: Discussionsupporting

confidence: 64%

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Staedler

Passemard

Magouroux

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

View full text Add to dashboard Cite

show abstract

“…Comparable iron oxide nanoparticles have frequently been used for MRI in animal models and in patients. In vitro studies have shown that high concentrations of nanoparticles may change the cytokine profile of macrophages, 25 yielding a dose-dependent shift toward an antiinflammatory phenotype was observed. It is unclear how the relatively high doses in this in vitro study reflect the in vivo situation.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle PET-CT Imaging of Macrophages in Inflammatory Atherosclerosis

et al. 2008

View full text Add to dashboard Cite

Background-Macrophages participate centrally in atherosclerosis, and macrophage markers (eg, CD68, correlate well with lesion severity and therapeutic modulation. On the basis of the avidity of lesional macrophages for polysaccharide-containing supramolecular structures such as nanoparticles, we have developed a new positron emission tomography (PET) agent with optimized pharmacokinetics to allow in vivo imaging at tracer concentrations. Methods and Results-A dextranated and DTPA-modified magnetofluorescent 20-nm nanoparticle was labeled with the PET tracer 64 Cu (1 mCi/0.1 mg nanoparticles) to yield a PET, magnetic resonance, and optically detectable imaging agent. Peak PET activity 24 hours after intravenous injection into mice deficient in apolipoprotein E with experimental atherosclerosis mapped to areas of high plaque load identified by computed tomography such as the aortic root and arch and correlated with magnetic resonance and optical imaging. Accumulated dose in apolipoprotein E-deficient aortas determined by gamma counting was 260% and in carotids 392% of respective wild-type organs (PϽ0.05 both). Autoradiography of aortas demonstrated uptake of the agent into macrophage-rich atheromata identified by Oil Red O staining of lipid deposits. The novel nanoagent accumulated predominantly in macrophages as determined by fluorescence microscopy and flow cytometry of cells dissociated from aortas. Conclusions-This report establishes the capability of a novel trimodality nanoparticle to directly detect macrophages in atherosclerotic plaques. Advantages include improved sensitivity; direct correlation of PET signal with an established biomarker (CD68); ability to readily quantify the PET signal, perform whole-body vascular surveys, and spatially localize and follow the trireporter by microscopy; and clinical translatability of the agent given similarities to magnetic resonance imaging probes in clinical trials. (Circulation. 2008;117:379-387.)

show abstract

“…These features are known to involve in general mechanism of acute inflammation caused not only by nanoparticulate magnetic substance but other external pollutant factors. The ability of micronized particles of various chemical nature and size to induce acute lung inflammation made in some reports supports this notion (Danilov et al, 2003;Siglienti et al, 2006;Fujiia et al, 2002;Schins et al, 2004;Donaldson et al, 2002;Wong et al, 2007).…”

Section: Resultsmentioning

confidence: 77%

“…The results of studying the protein composition by the HLPC and gel electrophoresis suggest transfer of a part of plasmatic proteins including antibodies into the alveolar area, which is a part of nonspecific protection of the organism caused by administration of NPs into bronchopulmonary tract. As a whole, the pattern of inflammatory processes with initiation of synthesis of pro-inflammatory cytokins and activation of the NF-kB complex corresponds to the pattern of nonspecific response of lung to inhalation of finely dispersed aerosols (Limbach et al, 2007;Oberdörster et al, 2005;Li et al, 1999;Tran et al, 2000;Danilov et al, 2003;Siglienti et al, 2006;Schins et al, 2004;Donaldson K. & Tran Cl, 2002;Zhu et al, 2008). The toxicity of iron NPs can be caused by the chemical composition of materials, the particular features of their size, increased aspiration dose of the preparation, and physiological peculiarities of their delivery through a respiratory tract.…”

Section: Discussionmentioning

confidence: 99%

The Inflammatory Response of Respiratory System to Metal Nanoparticle Exposure and Its Suppression by Redox Active Agent and Cytokine Therapy

Nikolaev¹,

Mikhalev²,

Marchenko³

et al. 2012

Recent Trend in Electrochemical Science and Technology

View full text Add to dashboard Cite

Cytokine profile of iron-laden macrophages: Implications for cellular magnetic resonance imaging

Cited by 85 publications

References 36 publications

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles

Nanoparticle PET-CT Imaging of Macrophages in Inflammatory Atherosclerosis

The Inflammatory Response of Respiratory System to Metal Nanoparticle Exposure and Its Suppression by Redox Active Agent and Cytokine Therapy

Contact Info

Product

Resources

About