Cytokine profile of patient’s sera of Leptospirosis to OMP of Leptospira interrogans serovar tarassovi

Abstract

Aims and Objectives: In the past decade, Leptospirosis has emerged as a globally important emerging infectious disease. Leptospires have several chemical components such as Lipoproteins, glycolipoproteins which are toxic and participate in the pathogenesis of the disease. This study was focused to find out the immune response of outer membrane proteins (OMPs) of Leptospira interrogans serovar tarassovi by estimating the cytokine profile and antibody levels from cases of Leptospirosis.Materials and Methods: A total of 120 blood samples were collected from patients (90 male/30 female) presenting with acute febrile illness and fulfilling the criteria of clinical diagnosis of Leptospirosis. All the 120 samples & 40 healthy controls were subjected to ELISA (IgM serion), MAT and Leptospira interrogans serovar tarassovi IgM ELISA and also to detect TNF? and IL-6 levels using (BD Opt EIA Human IL-6 & TNF? ELISA kits).Results: Sera which were found to be reactive in L. interrogans serovar tarassovi OMP IgM ELISA had significant levels of TNF? and IL-6 and antibody titres in ELISA and MAT.Conclusions: Patients from Group I had significant levels of TNF? in the serum i.e., p = 0.02 which indicates the pro-inflammatory Th1 response as TNF? are macrophage derived cytokine mediators. The IL-6 level of Group I, II, III were significantly higher than the Group IV (p = 0.02) suggesting that Th2 anti-inflammatory response seen in majority of these cases which has a protective role in leptospirosis.Asian Journal of Medical Sciences Vol.7(1) 2015 93-95

Cytokine response in human leptospirosis with different clinical outcomes: a systematic review

“…The full-texts of the remaining 43 articles were examined for suitability to the review criteria, and 25 were excluded. After the screening procedure, 18 articles were selected for the systematic review [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37]. Selected articles were published over 18 years from 2000 to 2018.…”

“…Most of the studies were conducted in India and Thailand (each 17% -3/18). The majority of the studies were comparative cross-sectional studies (83% -15/18 Studies reported Eotaxin-2 [22], G-CSF [22], GM-CSF [22,33], IFN-γ [22], IL-1β [22], IL-2 [35], IL-4 [22], IL-6 [21,22,26,31,33,34], IL-7 [22], IL-8 [21,23,26,31,33,36], IL-10 [21,22,26,31,36], IL-11 [22], IL-12P40 [28], IL-13 [22], IL-17 [22], IL-21 [20], IL-23 [20], IP-10 [27,33], MCP-1 [22,33], MIG [27], MIP-1δ [22], sTNFR1 [22], TNF-α [20-22, 31, 34, 35] and VEGF [33] as elevated among leptospirosis patients. In addition, studies also reported IL-1β [21,26], IL-12P70 [21,26] and TNFα [21,23,…”

“…An involvement of Th17 cells in the immunopathogenesis of leptospirosis was proposed by Bandara et al 2018 because an increase in IL-17A, IL-21 and IL-23 levels were observed [20]. Latha et al 2015 demonstrated significant levels of TNF-α and IL-6 in sera which were reactive in Leptospira tarassovi outer membrane protein IgM ELISA [34]. Wang et al 2012 revealed that the secretion of pro-inflammatory cytokines is induced by hemolysin of Leptospira interogans in human macrophages [22].…”