Cytokine profiles and clinical characteristics in primary Sjögren´s syndrome patient groups

López-Villalobos, Erika Fabiola; Muñoz‐Valle, José Francisco; Palafox‐Sánchez, Claudia Azucena; García‐Arellano, Samuel; Martínez‐Fernández, Diana Emilia; Orozco‐Barocio, Gerardo; García-Espinoza, José Antonio

doi:10.1002/jcla.23629

Cited by 6 publications

(7 citation statements)

References 25 publications

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“…These cells can produce both Th1 and Th2 cytokines but are characterized by a shift in the favor of Th1 in the salivary gland in patients with SS ( Mitsias et al, 2002 ). Moreover, an overexpression of the cytokines IL4, IL17, and IFNγ have been associated with the pathogenesis of SS ( Verstappen et al, 2017 ; López-Villalobos et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Proteomics Analysis of Tears and Saliva From Sjogren’s Syndrome Patients

et al. 2021

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Sjogren’s syndrome (SS) is characterized by dysfunctional mucous membranes and dysregulated moisture-secreting glands resulting in various symptoms, including dry mouth and dry eyes. Here, we wanted to profile and compare the tear and saliva proteomes of SS patients to healthy controls. Tear and saliva samples were collected and subjected to an isotopic dimethylation labeling shotgun proteomics workflow to identify alterations in protein levels. In tear samples, we identified 83 upregulated and 112 downregulated proteins. Pathway enrichment analysis of the changing proteins by Metascape identified leukocyte transendothelial migration, neutrophil degranulation, and post-translation protein phosphorylation in tears of SS patients. In healthy controls’ tears, an enrichment for proteins related to glycolysis, amino acid metabolism and apoptotic signaling pathway were identified. In saliva, we identified 108 upregulated and 45 downregulated proteins. Altered pathways in SS patients’ saliva included cornification, sensory perception to taste and neutrophil degranulation. In healthy controls’ saliva, an enrichment for proteins related to JAK-STAT signaling after interleukin-12 stimulation, phagocytosis and glycolysis in senescence were identified. Dysregulated protease activity is implicated in the initiation of inflammation and immune cell recruitment in SS. We identified 20 proteases and protease inhibitors in tears and 18 in saliva which are differentially expressed between SS patients and healthy controls. Next, we quantified endogenous proteoglycan 4 (PRG4), a mucin-like glycoprotein, in tear wash and saliva samples via a bead-based immune assay. We identified decreased levels of PRG4 in SS patients’ tear wash compared to normal samples. Conversely, in saliva, we found elevated levels of PRG4 concentration and visualized PRG4 expression in human parotid gland via immunohistological staining. These findings will improve our mechanistic understanding of the disease and changes in SS patients’ protein expression will help identify new potential drug targets. PRG4 is among the promising targets, which we identified here, in saliva, for the first time.

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Section: Introductionmentioning

confidence: 99%

Proteomics Analysis of Tears and Saliva From Sjogren’s Syndrome Patients

et al. 2021

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“…Serum containing unfavorable factors may even exacerbate ocular surface inflammation and lead to adverse effects when applied therapeutically. Some studies in the literature have demonstrated higher concentrations of serum inflammatory cytokines in patients with SS, especially when associated with a rheumatological disorder or during active disease (28,29,37,38). In a study by Ma et al (28), patients with SS were divided into active and inactive groups based on erythrocyte sedimentation rate and rheumatoid arthritis activity.…”

Section: Discussionmentioning

confidence: 99%

Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus

DUYAN¹,

Bozkurt

Ünlü

et al. 2023

Genel Tıp Dergisi

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Abstract Background/ Aims: Sjögren’s syndrome (SS) and diabetes mellitus (DM) are common causes of dry eye disease (DED) and autologous serum is used when artificial tears are not sufficient. Our aim was to compare serum growth factor and inflammatory cytokine levels of SS and DM patients with the healthy individuals. Methods: Twenty-three SS patients (mean age 52.70±7.55 years), 25 DM patients (mean age 56.68±6.53 years), and 23 healthy subjects (mean age 51.70±9.14 years) were included in the study. After detailed ophthalmological examination, Schirmer test, tear break-up time (TBUT) and Ocular Surface Disease Index (OSDI) scores were measured. Serum levels of six different proinflammatory interleukins (IL), five growth factors, matrix metalloproteinase-9, and fibronectin were measured by immunoassay. One-way ANOVA or Kruskal-Wallis tests and Dunn-Bonferroni post hoc analysis were used for comparison and p

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“… 105 Molecular markers might, in the future, provide key baseline information for pSS clustering. 42 , 45 , 106 …”

Section: Established Pssmentioning

confidence: 99%

Sjögren syndrome: looking forward to the future

Callegher

Giovannini

Zenz

et al. 2022

Therapeutic Advances in Musculoskeletal

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Primary Sjögren’s syndrome (pSS) is a heterogeneous disease characterised by a wide spectrum of manifestations that vary according to the different stages of the disease and among different subsets of patients. The aim of this qualitative literature review is to summarise the recent advances that have been reported in pSS, ranging from the early phases to the established disease and its complications. We analysed the diagnostic, prognostic, and management aspects of pSS, with a look into future clinical and research developments. The early phases of pSS, usually antedating diagnosis, allow us to investigate the pathophysiology and risk factors of the overt disease, thus allowing better and timely patient stratification. Salivary gland ultrasound (SGUS) is emerging as a valid complementary, or even alternative, tool for histopathology in the diagnosis of pSS, due to a standardised scoring system with good agreement and performance. Other promising innovations include the application of artificial intelligence to SGUS, ultrasound-guided core needle biopsy, and a wide array of novel diagnostic and prognostic biomarkers. Stratifying pSS patients through the integration of clinical, laboratory, imaging, and histopathological data; differentiating between activity-related and damage-related manifestations; and identifying patients at higher risk of lymphoma development are essential steps for an optimal management and individualised treatment approach. As new treatment options are emerging for both glandular and systemic manifestations, there is a need for a more reliable treatment response evaluation. pSS is a complex and heterogeneous disease, and many distinct aspects should be considered in the different stages of the disease and subsets of patients. In recent years, efforts have been made to improve our understanding of the disease, and certainly in the coming years, some of these novelties will become part of our routine clinical practice, thus improving the management of pSS patients.

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Cytokine profiles and clinical characteristics in primary Sjögren´s syndrome patient groups

Cited by 6 publications

References 25 publications

Proteomics Analysis of Tears and Saliva From Sjogren’s Syndrome Patients

Proteomics Analysis of Tears and Saliva From Sjogren’s Syndrome Patients

Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus

Sjögren syndrome: looking forward to the future

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