Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity

Boomsma, Carolien M.; Kavelaars, Annemieke; Eijkemans, Marinus J.C.; Amarouchi, Karima; Teklenburg, Gijs; Gutknecht, D; Fauser, Bcjm; Heijnen, Cobi J.; Macklon, Nick S.

doi:10.1016/s1472-6483(10)60429-4

Cited by 117 publications

(111 citation statements)

References 77 publications

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“…Interest in the protein content of endometrial secretions has gained much momentum in recent years and has been suggested to play a key role in the embryo implantation process. Therefore, differential proteomics of the eninsight to understand the mechanisms involved in the onset of endometrial pathologies and the process of embryo implantation [2].…”

Section: Communicationmentioning

confidence: 99%

Comprehensive Proteomic Analysis of Human Endometrial Fluid Aspirate

et al. 2009

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The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of endometrial function and reflects the state of the endometrium. In this study, we describe, for the first time, a comprehensive catalogue of proteins of the endometrial fluid during the secretory phase of the menstrual cycle. To achieve this objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein separation by denaturing one-dimensional electrophoresis (SDS-PAGE) followed by HPLC-MS/MS analysis. Finally, twodimensional polyacrylamide gel electrophoresis (2D-PAGE) followed by MALDI-TOF/TOF analysis. The combination of the three strategies led to the successful identification of 803 different proteins in the International Protein Index (IPI) human database (v3.48). An extensive description of the endometrial fluid proteome will help provide the basis for a better understanding of a number of diseases and processes, including endometriosis, endometrial cancer and embryo implantation. We believe that the thorough catalogue of proteins presented here can serve as a valuable reference for the study of embryo implantation and for future biomarker discovery involved in pathologic alterations of endometrial function.

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Section: Communicationmentioning

confidence: 99%

Comprehensive Proteomic Analysis of Human Endometrial Fluid Aspirate

et al. 2009

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“…This issue should be addressed based on the analysis to be undertaken. For instance, blood contamination recovered from ALF samples in women was a confounder factor when analysing pro-inflammatory cytokines since red cells interfered with the measurements of pro-inflammatory cytokines in uterine fluid aspirated from women (Boomsma et al, 2009b).…”

Section: Discussionmentioning

confidence: 99%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

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In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

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“…Badania takie mogłyby być niezwykle użyteczne przy określaniu optymalnego czasu transferu zarodka do macicy w procedurze IVF, jeszcze w tym samym cyklu, w którym przeprowadzono badanie wydzieliny. Dalsze badania powinny skupić się na wyjaśnieniu zmian poziomu poszczególnych markerów podczas cyklu miesięcznego kobiety, dopuszczalnej zmienności w poziomach markerów pomiędzy cyklami i opracowania zakresu wartości prawidłowych poszczególnych markerów w poszczególnych fazach cyklu [27,28].…”

Section: Opis Stanu Wiedzyunclassified

“…[27] dokonali analizy składu cytokin w wydzielinie macicy oraz śluzu szyjkowego kobiet w okresie receptywności doczesnej. Wśród wykrytych substancji znalazło się wiele czynników o różnych funkcjach i właś-ciwościach, np.…”

Section: Opis Stanu Wiedzyunclassified

The role of immunological markers in assessment of implantation uterus receptivity - clinical implications.

Macura¹,

Szczepanik²,

Sura³

et al. 2017

Med Og Nauk Zdr.

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Macura B, Szczepanik M, Sura P, Śliwa L. Rola markerów immunologicznych w ocenie właściwości implantacyjnych endometrium macicyimplikacje kliniczne. Med. Og Nauk Zdr. 2017; 23(2): 143-147. doi: 10.26444/monz/75293 Streszczenie Wprowadzenie i cel pracy. Problemy nawracających poronień u kobiet, jak również niepowodzeń w implantacji wszczepianych zarodków w metodzie zapłodnienia in vitro (IVF) mogą być związane z nieprawidłowym funkcjonowaniem błony śluzowej macicy. Do przyczyn tych zjawisk zalicza się m.in. nieprawidłowości immunologiczne w funkcjonowaniu endometrium. Skrócony opis stanu wiedzy. Implantację można zdefiniować jako proces wnikania zarodka w głąb błony śluzowej macicy. Przebieg procesu implantacji jest uzależniony od wielu molekularnych interakcji pomiędzy "receptywnym" endometrium a dojrzałą blastocystą. Dojrzała blastocysta może rozpocząć implantację w endometrium macicy tylko podczas tzw. "okienka implantacyjnego". Ograniczony czas "okienka implantacyjnego" umożliwia skoordynowaną komunikację molekularną pomiędzy zarodkiem a endometrium. Obecnie znanych jest wiele czynników pochodzenia macicznego i zarodkowego związanych z implantacją i wczesnym utrzymaniem ciąży. Niestety, chociaż teoretyczna znajomość procesów immunologicznych w endometrium macicy jest powszechnie znana, to jednak trudno jest przełożyć tę wiedzę na praktykę kliniczną. Podsumowanie. Istnieje nadzieja, że nowe procedury diagnostyczne tych zaburzeń będą opierać się o bezpośredni pomiar stężeń określonych markerów w wydzielinie endometrium. Być może w niedługiej przyszłości wiedza o immunologicznych zaburzeniach endometrium zostanie lepiej wykorzystana, co umożliwi skuteczniejszą terapię par z problemem niepłodności. Słowa kluczoweczynniki immunologiczne, endometrium, implantacja, poronienia nawykowe, zapłodnienie in vitro (IVF)

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Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity

Cited by 117 publications

References 77 publications

Comprehensive Proteomic Analysis of Human Endometrial Fluid Aspirate

Comprehensive Proteomic Analysis of Human Endometrial Fluid Aspirate

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

The role of immunological markers in assessment of implantation uterus receptivity - clinical implications.

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