Abstract:When injected subcutaneously, extracts from the white berry mistletoe (Viscum album L) lead to a dose-dependent local inflammatory reaction at the injection site. From in vitro investigations with V album extracts, the release of proinflammatory cytokines from peripheral blood mononuclear cells and from a human skin model (Skin(2) model; Advanced Tissue Sciences, La Jolla, CA) is known. This shows dose dependence for mistletoe lectin-I in the range of 0.02 ng/mL to 10.0 ng/mL. In this study, an investigation w… Show more
“…In a skin model system, VAA-I (0.75–8 ng/ml) given in isolated form or in mistletoe extracts caused increased release of IL-1α and IL-6 dose dependently (44). In a model of multilayered keratinocytes, similar results were found (45). Proinflammatory cytokines play a significant role in the regulation of innate immunity.…”
Section: Biological Activity Of Mistletoe Lectin (Vaa-i)supporting
“…In a skin model system, VAA-I (0.75–8 ng/ml) given in isolated form or in mistletoe extracts caused increased release of IL-1α and IL-6 dose dependently (44). In a model of multilayered keratinocytes, similar results were found (45). Proinflammatory cytokines play a significant role in the regulation of innate immunity.…”
Section: Biological Activity Of Mistletoe Lectin (Vaa-i)supporting
“…European mistletoe lectin caused increased secretion of TNF-α, IL-1, and IL-6 in exposed human mononuclear cells (13). Experiments using multilayered skin keratinocytes showed that European mistletoe extracts caused the release of IL-1α, IL-2, IL-6, IL-10 and TNF-α (12). Thus, the present study suggests ML-J to have very similar properties to European and Korean mistletoe lectins so that ML-J can be expected to have anti-cancer activity in vivo.…”
Section: Effects Of Ml-j On Gene Expression In Caco-2 Cellssupporting
Mistletoe lectins have various biological activities including anti-cancer and immunomodulatory effects. We previously isolated a lectin (ML-J) from Japanese mistletoe. In the present study, we examined the effects of ML-J on cytokine gene expression in human colon adenocarcinoma Caco-2 cells and in the mouse intestine. The results of reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction indicated that ML-J caused an upregulation of the gene expression of the proinflammatory cytokines interleukin (IL)-8, tumor necrosis factor-α (TNF-α) and IL-6 in Caco-2 cells and TNF-α and IL-6 in the duodenum. This study provides the first example to show that a perorally administered plant lectin affects gene expression in the duodenum.
“…Conversely, the effects of different stimuli on the metabolic activity of macrophages and the secretion of proinflammatory mediators are clearly not superimposable [78,79]. It may also be the case that the secretion of proinflammatory mediators is increased after the inhibition of the metabolic activity of cells [80,81].…”
Section: Functional Properties Of Sst 1 and Sst 2 In Human Macrophagesmentioning
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