Cytokine-Stimulated Human Natural Killer Cytotoxicity: Response to Rotavirus-Infected Cells

Kohl, Steve; Harmon, Maurice W.; Tang, J.-P.

doi:10.1203/00006450-198311000-00006

Cited by 15 publications

(11 citation statements)

References 10 publications

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“…Also, cytotoxic activity in our study was demonstrated for IEL preparations from uninoculated control chickens which were negative for antibodies to rotaviruses. While Kohl et al (1983) reported NK activity for human peripheral blood leukocytes against rotavirus-infected target cells, our demonstration of IEL cytotoxicity for rotavirus-infected cells extend these findings to suggest that NK cell activity may indeed be an important intestinal response to rotavirus infection. In similar studies, in vitro spontaneous cytotoxic activity has been demonstrated for murine (Carman et al, 1986) and porcine (Cepica and Derbyshire, 1983 ) IELs against emetic coronavirus-infected target cells.…”

Section: Discussionsupporting

confidence: 78%

“…This cytotoxic activity was directed against target cells infected with rotaviruses either homotypic or heterotopic to the virus inoculum. Kohl et al (1983) demonstrated that human interferon can induce in vitro NK cell activity of human peripheral blood leukocytes (collected from randomly selected individuals) against rotavirus-infected target cells. In calves, La Bonnardiere et al (1981) showed that intestinal interferon is produced during rotavirus infection and Van den Broecke et al (1984) found a correlation between high levels of intestinal interferon and reduced clinical disease.…”

Section: Introductionmentioning

confidence: 99%

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Natural killer cell activity of chicken intraepithelial leukocytes against rotavirus-infected target cells

Myers

Schat

1990

Veterinary Immunology and Immunopathology

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Intraepithelial leukocytes (IEL) and splenocytes collected from uninfected and rotavirus-infected chickens were evaluated for cytotoxic activity against a natural killer (NK) cell-susceptible lymphoblastoid cell line (LSCC-RP9) and against rotavirus-infected chick kidney cells in 4-h chromium-release assays. Both splenocytes and IELs from uninfected and rotavirus-infected chickens were cytotoxic for LSCC-RP9, and the levels of this NK cell activity were not altered by infection of the host with rotavirus. IELs but not splenocytes from uninfected and rotavirus-infected chickens were cytotoxic for rotavirus-infected but not for uninfected chick kidney cell targets. Because this cytotoxic activity was not induced nor altered by rotavirus infection of the host, and was not major histocompatibility complex-restricted, it was considered to be due to NK cell activity. The cytotoxicity of IELs against rotavirus-infected target cells was dose-dependent; however, there was some suppression of cytotoxic activity at high effector to target cell ratios. There were no differences in the cytotoxic activities of IELs collected from the duodenum versus the jejunum. The in vitro cytotoxic activity of IELs against rotavirus-infected target cells suggested that NK cell activity may be an important immune response to rotavirus infections in vivo. The absence of cytotoxic activity by splenocytes against rotavirus-infected target cells indicated that there may be different subpopulations of NK cells in the spleen and intestinal epithelium of chickens.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Natural killer cell activity of chicken intraepithelial leukocytes against rotavirus-infected target cells

Myers

Schat

1990

Veterinary Immunology and Immunopathology

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“…Cytokines released by RVactivated CD4 ϩ T cells, such as interferons (IFNs) might activate intraepithelial natural killer cells which, in turn, would kill RV-infected epithelial cells. Thus, IFN was shown to activate cytotoxicity of human natural killer cells against RVinfected target cells (19). On the other hand, IFN-␥ released from activated natural killer cells (especially in the SCID microenvironment) or CD4 ϩ T cells themselves might kill infected epithelial cells directly.…”

Section: Our Findings That Appreciable Numbers Of Cd4mentioning

confidence: 99%

B2 but Not B1 Cells Can Contribute to CD4⁺T-Cell-Mediated Clearance of Rotavirus in SCID Mice

Kushnir

Bos

Zuercher

et al. 2001

J Virol

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“…It has been reported that various kinds of virus-infected cells are susceptible to lysis by NK and LAK cells (Santoli et al. 1978: Kohl et al. 1983Yasukawa & Zarling.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus induces proliferative response and augments non-specific cytotoxic activity of lymphocytes in humans

Yasukawa

Nakagomi

Kobayashi

1990

Clinical and Experimental Immunology

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SUMMARYIn vitro ccll-mediated immune responses lo rotavirus in humans were studied. Peripheral blood mononuclear cells (PBMC) of healthy adults proliferated in response to stimiihuion with the infectious and u.v.-inactivated Wa strain othuman rotavirus, showing a maximum response on day 7 of culture; however, cord blood lymphocytes failed to respond to rotavirus. A CTOSS-reactive proiiferative response of PBMC detected by stimulation with the NCDV strain of bovine rotavirus suggests the existence of epitopes common to both human and bovine rotaviruses. which are recognized by human T lymphocytes. The phenotype of the majority of activated lymphocytes was CD3 *4* 8 , indicating that the cells mainly activated were helper T cells. Culture supernatants of PBMC stimulated with rotavirus contained interleukin-2 (IL-2) and interferon-gamma (IFN-y). In addition, PBMC stimulated with rotavirus demonstrated significantly enhanced cytotoxic activity against natural killer (NK) sensitive K562 ceils as well as an NK-resistanl Epstein-Barr virusimmortalized lymphoblastoid cell line (LCL). Treatment of PBMC with anti-CDl6 or NKHIA monoclonal antibody, both of which react with most NK cells and lymphokine-activated killer cells and complement markedly reduced the cytotoxic activity against K562 and LCL. These results suggest that stimulation of human PBMC with rotavirus results in the prodtiction of tymphokines, such as IL-2 and IFN-y, by rotavirus-reactive helper T cells and that these lymphokines augment NK activity and generate other forms of non-specific eytotoxic human lymphocyte activity. These cellmediated immune responses observed in the present in vitro study might play an important role in protection and recovery from rotavirus infection.

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Cytokine-Stimulated Human Natural Killer Cytotoxicity: Response to Rotavirus-Infected Cells

Cited by 15 publications

References 10 publications

Natural killer cell activity of chicken intraepithelial leukocytes against rotavirus-infected target cells

Natural killer cell activity of chicken intraepithelial leukocytes against rotavirus-infected target cells

B2 but Not B1 Cells Can Contribute to CD4⁺T-Cell-Mediated Clearance of Rotavirus in SCID Mice

Rotavirus induces proliferative response and augments non-specific cytotoxic activity of lymphocytes in humans

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Cytokine-Stimulated Human Natural Killer Cytotoxicity: Response to Rotavirus-Infected Cells

Cited by 15 publications

References 10 publications

Natural killer cell activity of chicken intraepithelial leukocytes against rotavirus-infected target cells

Natural killer cell activity of chicken intraepithelial leukocytes against rotavirus-infected target cells

B2 but Not B1 Cells Can Contribute to CD4+T-Cell-Mediated Clearance of Rotavirus in SCID Mice

Rotavirus induces proliferative response and augments non-specific cytotoxic activity of lymphocytes in humans

Contact Info

Product

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B2 but Not B1 Cells Can Contribute to CD4⁺T-Cell-Mediated Clearance of Rotavirus in SCID Mice