“…Immunofluorescent labeling and microscopic imaging has been described previously. 16 Primary antibodies used were ßIIItubulin (ab78078, 1:250; Abcam, Cambridge, MA), calbindin-D28K (C2724, 1:500; Sigma-Aldrich, St Louis, MO), CD11b/ c (ab1211, 1:100, Abcam), EGFP (ab6556, 1:500, Abcam), glial fibrillary acidic protein (GFAP; ab33922, 1:200, Abcam), IBA-1 (ab5076, 1:200, Abcam), NeuN (ab177487, 1:500 and ab104224, 1:500, Abcam), S100 (MAB079, 1:200; Millipore, Billerica, MA), S100 (Z0311, 1:200; Dako, Carpinteria, CA). Secondary antibodies were Alexa Fluor 488/555, goat/donkey antimouse (1:500), Alexa Fluor 488/555, goat/donkey anti-rabbit Experimental protocol using wild-type (WT) and YG8R mice to investigate the effects of allogeneic BMT.…”