Cytokine TNF-α and its receptors TNFRI and TNFRII play a key role in the in vitro proliferative response of BLV infected animals

Lendez, Pamela Anahí; Martinez-Cuesta, Lucía; Farías, María Victoria Nieto; Dolcini, Guillermina Laura; Ceriani, María Carolina

doi:10.1007/s11259-021-09825-z

Vet Res Commun

2021

DOI: 10.1007/s11259-021-09825-z

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Cytokine TNF-α and its receptors TNFRI and TNFRII play a key role in the in vitro proliferative response of BLV infected animals

Pamela Anahí Lendez

Lucía Martinez-Cuesta

María Victoria Nieto Farías

et al.

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Cited by 4 publications

(1 citation statement)

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“…The conditions were as follows: 95°C for 1 min, 95°C, 20 s, and 60°C, 1 min, for 40 cycles. The primer sequences of the genes are shown in Table 2(Cui et al, 2020;Lendez et al, 2021).…”

mentioning

confidence: 99%

NF-κB signaling pathway mechanism in cow intertoe skin inflammation caused by Fusobacterium necrophorum

Zhang

Zheng

Sun

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundFusobacterium necrophorum is the main pathogen inducing bovine foot rot. The infected site is often accompanied by a strong inflammatory response, but the specific inflammatory regulatory mechanism remains unclear.AimA cow skin explants model was established to elucidate the mechanism of F. necrophorum bacillus causing foot rot in cows, and to provide reference for future clinical practice.MethodsCow intertoe skin explants were cultured in vitro, and F. necrophorum bacteria solution and nuclear factor-κB (NF-κB) inhibitor BAY 1-7082 were added to establish an in vitro infection model. Hematoxylin and eosin staining, terminal - deoxynucleotidyl transferase mediated nick end labeling, and immunohistochemistry were used to detect the pathological changes of the skin explants infected with F. necrophorum, the degree of tissue cell apoptosis, and the expression of the apoptosis-related protein Caspase-3, respectively. RT-qPCR, Western blot, and ELISA were used to detect the activation of the NF-κB pathway and inflammatory cytokines by F. necrophorum.ResultsThe intertoe skin structure of cows infected with F. necrophorum changed with different degrees of inflammation, and the degree of tissue cell apoptosis was significantly increased (P < 0.01). In addition, infection with F. necrophorum significantly increased the phosphorylation level of IκBα protein and up-regulated the expression level of NF-κB p65. The high expression and transcriptional activity of NF-κB p65 significantly increased the expression and concentration of the inflammatory cytokines TNF-α, IL-1β, and IL-8, thus inducing the occurrence of an inflammatory response. However, inhibition of NF-κB p65 activity significantly decreased the expression of inflammatory factors in the intertoe skin of cows infected with F. necrophorum.ConclusionF. necrophorum activates NF‐κB signaling pathway by increasing the expression of TNF‐α, IL‐1β, IL‐8 and other inflammatory factors, leading to foot rot in dairy cows.

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“…The conditions were as follows: 95°C for 1 min, 95°C, 20 s, and 60°C, 1 min, for 40 cycles. The primer sequences of the genes are shown in Table 2(Cui et al, 2020;Lendez et al, 2021).…”

mentioning

confidence: 99%

NF-κB signaling pathway mechanism in cow intertoe skin inflammation caused by Fusobacterium necrophorum

Zhang

Zheng

Sun

et al. 2023

Front. Cell. Infect. Microbiol.

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An LPS-induced TNF-α factor involved in immune response of oyster Crassostrea gigas by regulating haemocytes apoptosis

Tang,

Qiao,

Jin

et al. 2024

Fish & Shellfish Immunology

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Altered apoptosis and proliferation in milk cells and PBMc from BLV-infected bovines with different proviral loads: Possible role of the BCL-2 family proteins, TNF-alpha, and receptors

Ladera Gómez,

Nieto Farias,

Rodríguez

et al. 2024

Veterinary Immunology and Immunopathology

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Cytokine TNF-α and its receptors TNFRI and TNFRII play a key role in the in vitro proliferative response of BLV infected animals

Cited by 4 publications

References 57 publications

NF-κB signaling pathway mechanism in cow intertoe skin inflammation caused by Fusobacterium necrophorum

NF-κB signaling pathway mechanism in cow intertoe skin inflammation caused by Fusobacterium necrophorum

An LPS-induced TNF-α factor involved in immune response of oyster Crassostrea gigas by regulating haemocytes apoptosis

Altered apoptosis and proliferation in milk cells and PBMc from BLV-infected bovines with different proviral loads: Possible role of the BCL-2 family proteins, TNF-alpha, and receptors

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