Cytokines and Markers of Immune Response to HPV Infection

Koshiol, Jill; Kovacic, Melinda Butsch

doi:10.5772/32540

Cited by 4 publications

(5 citation statements)

References 52 publications

(57 reference statements)

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“…Its primary purpose was to evaluate the performance of a commercially available multiplexed immune panel in the analysis of cervical secretions. Because this study was not specifically powered to make comparisons by HPV and CIN status, although its size is comparable to the majority of studies that have measured immune markers in cervical secretions, 12–16 true differences may have been missed. Given the number of immune marker and CIN/HPV comparisons, some results may have been due to chance.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies of cytokines in the context of HPV infection or cervical precancer or cancer typically have assessed only a few cytokines. 12–16 New developments now make it feasible to measure many immune-related markers simultaneously in a high-throughput, multiplex format using small volumes of specimen. Measuring proteins that are secreted from immune cells like cytokines, chemokines, and soluble receptors, which affect cell-mediated immune response and can be altered in the presence of infection, 17–19 may help detect early changes in the immune response that predict whether an HPV infection will progress to cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a multiplex panel of immune‐related markers in cervical secretions: A methodologic study

Koshiol

Sklavos

Wentzensen

et al. 2013

Intl Journal of Cancer

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While persistent carcinogenic human papillomvirus (HPV) infection is necessary for cervical carcinogenesis, the co-factors involved in HPV persistence and disease progression are poorly understood. Chronic cervical inflammation may increase risk, but few studies have measured immune markers (cytokines/chemokines/soluble receptors) in cervical secretions. We evaluated the performance of 74 multiplexed, bead-based immune markers in cervical secretions from three groups of women with biopsy evaluation of cervical intraepithelial neoplasia (CIN): 1) 25% detectability and >80% interclass correlation coefficients (ICC) acceptable for epidemiologic studies. Within-batch coefficients of variation (CV) of ≥25% indicated room for assay improvement. Secondarily, we explored associations between marker levels and CIN/HPV status adjusted for matching variables, assay batch, age, and number of sexual partners. Sixty-two markers (84%) had >25% detectability and ICCs>80%. Of those, 53 (85%) had CVs<25%. Using these preliminary data, we found that HPV-positivity was associated with increased eotaxin-1 (OR: 15.63, 95% CI: 1.26–200.00) and G-CSF (OR: 12.99, 95% CI: 1.10–142.86) among CIN-negative women. There was suggestive evidence that higher chemoattractant marker levels were associated with CIN2/3 (e.g., MIP-1delta, OR: 4.48, 95% CI: 0.87–23.04 versus

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of a multiplex panel of immune‐related markers in cervical secretions: A methodologic study

Koshiol

Sklavos

Wentzensen

et al. 2013

Intl Journal of Cancer

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“…(47) Other studies have shown that plasma IL-2 levels can differ depending on cervical HPV positivity and/or disease stage, although results have varied. (13) Our finding that IL-2 is related to decreased clearance in men may have been affected by its lower detectability, and may not represent true sex differences in IL-2. Therefore, the association of IL-2 with oral HPV clearance observed in this study warrants confirmation from other studies.…”

Section: Discussionmentioning

confidence: 81%

“…(7, 8) Attempts to characterize these responses have found that women with persistent cervical HPV have distinct systemic and/or local cytokine profiles as compared to women who clear HPV or women without HPV infection. (9-13) In particular, higher circulating or local levels of interleukin (IL)-6, IL-8 and tumor necrosis factor-alpha (TNF-α) have been associated with reduced cervical HPV clearance,(9-11) suggesting that cytokines secreted may be predictive of disease progression and outcome, although results have been mixed. (14, 15) The relationships between cytokines and oral HPV infection, and any differences by biologic sex, have not been described.…”

Section: Introductionmentioning

confidence: 99%

Association of serum cytokines with oral HPV clearance

et al. 2016

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Background Initial studies suggest higher serum levels of some pro-inflammatory cytokines may be associated with decreased cervical human papillomavirus (HPV) clearance. However, the relationship of cytokines with oral HPV clearance has not been explored. Methods From 2010 to 2014, oral rinse and serum samples were collected semi-annually from 1,601 adults. Oral rinse samples were tested for HPV DNA using PCR. Based on oral HPV results, 931 serum samples were selected for cytokine evaluation to include a roughly equal number of prevalent (n=307), incident (n=313), and no oral HPV infections (n=311). Electrochemiluminescence multiplex assays were used to determine the concentrations of IL-6, IL-8, TNF-α, IFN-γ, IL-1β, IL-2, IL-4, IL-10, IL-12 and IL-13. The relationship between serum cytokine concentrations (categorized into quartiles) and oral HPV clearance was evaluated with Wei-Lin-Weissfeld regression models, adjusting for HPV infection type (prevalent vs. incident), age, HIV status, and CD4 T cell count. Results Higher TNF-α concentration was associated with decreased clearance in men (highest vs. lowest quartile, adjusted hazard ratio [aHR]=0.52, 95% CI=0.34-0.79) and women (aHR=0.76, 95% confidence interval [CI]=0.55-1.04), with stronger associations in men than women (p-interaction=0.049). Higher IL-2 concentration was associated with reduced clearance in men (aHR=0.69, 95% CI=0.50-0.95), but not women (p-interaction=0.058). Results were similar within CD4 T cell strata (CD4 ≥500 or CD4<500 cells/μl) among HIV-infected participants. No other cytokines were associated with clearance. Conclusion High serum TNF-α is associated with reduced clearance of oral HPV infection.

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“…Entretanto, grande parte das infecções por HPV são eliminadas com sucesso pelo sistema imune, principalmente através da resposta imune adaptativa celular. Os tipos HPV16 e HPV18 persistem por mais tempo que os demais tipos de alto risco, o que explica, em parte, a discrepante prevalência destes tipos em carcinomas (Koshiol & Kovacic, 2012).…”

Section: Imunologia Das Infecções Por Hpvunclassified

Vias de transdução de sinal e polimorfismo de <i>Toll-like Receptors</i> na carcinogenese por HPV

Oliveira¹

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Cytokines and Markers of Immune Response to HPV Infection

Cited by 4 publications

References 52 publications

Evaluation of a multiplex panel of immune‐related markers in cervical secretions: A methodologic study

Evaluation of a multiplex panel of immune‐related markers in cervical secretions: A methodologic study

Association of serum cytokines with oral HPV clearance

Vias de transdução de sinal e polimorfismo de <i>Toll-like Receptors</i> na carcinogenese por HPV

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