Cytokines as Immunological Adjuvants

Aw, Heath

doi:10.1007/978-1-4615-1823-5_28

Cited by 54 publications

(34 citation statements)

References 53 publications

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“…Rabies virus and herpes simplex virus vaccines supplemented with interleukin-2 (IL-2) were found to be successful in animals at about the same time (50,76). Subsequently, other cytokines have been evaluated as adjuvants in a variety of experimental vaccines (22,23,25,26). In a related approach, naked DNA or recombinant vaccine strains have been engineered to express soluble cytokines upon administration to the host (6,7,24,27,35,36).…”

mentioning

confidence: 99%

General Strategy for Decoration of Enveloped Viruses with Functionally Active Lipid-Modified Cytokines

Kueng

Leb

Haiderer

et al. 2007

J Virol

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Viral particles preferentially incorporate extra-and intracellular constituents of host cell lipid rafts, a phenomenon central to pseudotyping. Based on this mechanism, we have developed a system for the predictable decoration of enveloped viruses with functionally active cytokines that circumvents the need to modify viral proteins themselves. Human interleukin-2 (hIL-2), hIL-4, human granulocyte-macrophage colony-stimulating factor (hGM-CSF), and murine IL-2 (mIL-2) were used as model cytokines and fused at their C terminus to the glycosylphosphatidylinositol (

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mentioning

confidence: 99%

General Strategy for Decoration of Enveloped Viruses with Functionally Active Lipid-Modified Cytokines

Kueng

Leb

Haiderer

et al. 2007

J Virol

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“…The Th1 response is characterized by γ-interferon, granulocyte monocyte colony stimulating factor, and interleukin-12 activates macrophages and cytotoxic T cells [179]. The direct approach of using cytokines as vaccine adjuvants fi rst concentrated on three cytokines, interleukin-1, interleukin-2, and γ-interferon [180], followed by successful application of interleukin-12 to leishmania vaccine [181] and use of granulocyte monocyte colony-stimulating factor [182] with both peptide and protein tumor antigens. The most extensive efforts on infectious disease vaccines were made with interleukin-2, a T-cell-activating agent that was used alone with rabies vaccines, where it increased protection 25-to 50-fold [183,184] and in combination with several vehicles to be discussed later.…”

Section: Historical Progressionmentioning

confidence: 99%

Development of Vaccine Adjuvants: A Historical Perspective

Ott

Nest

2006

Vaccine Adjuvants and Delivery Systems

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“…Exogenous IFN-γ can be administered alone in the treatment of diseases alone or together with genetically engineered vaccines to enhance and improve their immune effect (Sun et al 2005). Due to its central role in orchestrating the immune response, it has become an attractive candidate for use as a vaccine adjuvant (Heath & Playfair 1992), growth promotion in poultry and livestock (Lowenthal et al 2000), and potential alternatives to antibiotics (Lillehoj et al 1992;Digby et al 1995;Song et al 1997;Lowenthal et al 1997Lowenthal et al , 1998Yashiro et al 2001;Takehara et al 2003). Furthermore, IFNs are non-toxic after administration into the nose or mouth (oromucosal route) or the stomach (intragastric route) at different doses and under different administration conditions (Cummins et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression of synthetic chicken IFN-γ in transgenic tobacco plants

Zhao

Song

et al. 2009

Biologia

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To develop a plant expression system for the production of the chicken interferon gamma (ChIFN-γ) oral vaccine adjuvant, we investigated whether the ChIFN-γ protein can be expressed in tobacco plants. The coding sequence of the ChIFN-γ gene was optimized by modification of codon usage to that of tobacco plant genes. A synthetic ChIFN-γ gene was inserted into plasmid pSW-IFNG containing the CaMV35S promoter, NOS terminator, GUS (β-glucuronidase) reporter gene and the nptII resistance gene. The synthetic ChIFN-γ gene, along with an endoplasmic reticulum retention signal (SEKDEL) was introduced into tobacco plants via Agrobacterium-mediated transformation. PCR analysis confirmed the presence of the ChIFN-γ gene in transformants. Histochemical GUS assay of the tobacco leaves revealed stable integration of ChIFN-γ into the genome. RT-PCR analysis revealed the presence of ChIFN-γ-specific transcript. Bands of approximately 30 and 40 kDa were detected by Western analysis of transgenic tobacco leaves using an anti-chicken IFN-γ antibody. Furthermore, quantitative ELISA detected ChIFN-γ protein, suggesting that tobacco leaves expressed ChIFN-γ of up to 10 to 20 µg/g fresh leaf weight, or 0.02 to 0.04% of total soluble protein. ChIFN-γ present in tobacco extracts possessed antiviral activity (4.8×105 IU/mg in vitro). The present results indicate that the ChIFN-γ introduced into tobacco plant was correctly transcribed and translated in plant. High-level expression of biologically active ChIFN-γ will allow further studies of oral administration and therapeutic effects of this cytokine.

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Cytokines as Immunological Adjuvants

Cited by 54 publications

References 53 publications

General Strategy for Decoration of Enveloped Viruses with Functionally Active Lipid-Modified Cytokines

General Strategy for Decoration of Enveloped Viruses with Functionally Active Lipid-Modified Cytokines

Development of Vaccine Adjuvants: A Historical Perspective

Heterologous expression of synthetic chicken IFN-γ in transgenic tobacco plants

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