Cytokines, estradiol and progesterone in the plasma of women of reproductive age with pelvic inflammatory  disease in remission

Трунов, А. Н.; Обухова, О. О.; Горбенко, О. М.; Shvayk, Alya; Trunova, L. A.

doi:10.4236/abb.2013.46096

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…As we known, IL-1 β and IL-6 were two main proinflammatory cytokines. In upper genital tract of PID patients, elevated IL-1 β could increase IL-6 level and in turn suppress the IL-1 β synthesis in the second phase of the immune response [ 28 , 29 ]. In upper genital tract, these proinflammatory cytokines can induce chemokines (CXCL-1, MCP-1) production which lead to hematopoietic immune cells recruitment and stimulate the proliferation and activation of leukocyte.…”

Section: Discussionmentioning

confidence: 99%

Anti‐Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF‐κB Pathway

Liu

Yang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Objectives In this study, we aimed to illustrate the preventive effect and possible mechanisms of Feiyangchangweiyan capsule (FYCWYC) on rat pelvic inflammatory disease (PID) model. Methods To construct the rat PID model, upper genital tract was infected by multipathogen, and then drugs were orally administered for 8 days. The histological examination, immunohistochemical analysis, and ELISA were carried out. Furthermore, Western blotting was used to analyze the expression of Akt, MAPKs, NF-κB p65, and IκB-α in uterus. Results As the results showed, infiltrations of neutrophils and lymphocytes in uterus were significantly suppressed, and IL-1β, IL-6, CXCL-1, and TNF-α were also reduced in a dose-dependent manner. We also found that FYCWYC inhibited apoptosis induced by infection. Furthermore, FYCWYC could block the infection-induced nuclear translocation of NF-κB. We found that FYCWYC treatment only decreased the phosphorylation of JNK induced by infection and had no effects on Akt and P38. Additional, the effects of SP600125, an inhibitor of phospho-JNK, were similar to the results of FYCWYC. Conclusions Taken together, our results demonstrated that FYCWYC had anti-inflammatory effect in pathogen-induced PID model, and the mechanism might be through inhibiting NF-κB nuclear translocation which is mediated by JNK.

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Section: Discussionmentioning

confidence: 99%

Anti‐Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF‐κB Pathway

Liu

Yang

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…2A ) illustrated the inflammatory response. The pro-inflammatory cytokine IL-1β is a key factor in the pathological development of PID ( 25 ), and the cytokine IL-6 is involved in the processes of chronization and suppression of the synthesis of IL-1β in the second phase of the immune response ( 26 ). Both IL-1β and IL-6 exhibit significant elevation in patients with PID ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

Gas chromatography-mass spectrometric method-based urine metabolomic profile of rats with pelvic inflammatory disease

Zou

Wen

Sheng

et al. 2016

Experimental and Therapeutic Medicine

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Pelvic inflammatory disease (PID) can lead to a poor outcome of severe sequelae, and the current methods of clinical diagnosis are not satisfactory. Metabolomics is an effective method for the identification of disease-related metabolite biomarkers to facilitate disease diagnosis. However, to the best of our knowledge, no PID-associated metabolomic study has yet been carried out. The metabolomic changes of rats with PID were investigated in the present study. A PID model was constructed by the multi-pathogenic infection of the upper genital tract in rats. Infiltration of inflammatory cells and elevated expression levels of the cytokines interleukin (IL)-1β and IL-6 in the uterus and fallopian tubes validated the disease model. Gas chromatography-mass spectrometry coupled with derivatization was used to determine the urine metabolomic profile. Principal component analysis and partial least squares-discriminant analysis of the data sets showed a clear separation of metabolic profiles between rats with PID and control rats. Eighteen differentiating metabolites were found, including four amino acids, three fatty acids, nine organic acids, and two sugars, which indicated alterations in sugar metabolism, the citric acid cycle, amino acid metabolism and fatty acid metabolism. These metabolites could be potential biomarkers of PID, and this research may offer a new approach to evaluate the effect of anti-PID drugs in pre-clinical or clinical trials.

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“…As the plasma CRP level has been included in the criteria for clinical diagnosis of PID [ 35 ], we also observed the concentration changes of CRP to illustrate the inflammation in PID group and anti-inflammatory effect of PSF extract. Elevated IL-1 β in upper genital tract indicates the pathological development of PID [ 36 ], while increasing production of IL-6 is involved in the processes of chronization and can suppress the synthesis of IL-1 β in the second phase of the immune response [ 37 ]. In our study, the concentrations of IL-1 β and IL-6 in uterus and fallopian tube were significantly deceased in PSF group compared with PID group, indicating the anti-inflammatory effect of PSF extract.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Study on the Preventive Effect ofPatrinia scabiosaefoliaFisch on Multipathogen Induced Pelvic Inflammatory Disease in Rats

Zou

Wen

Zheng

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Patrinia scabiosaefolia Fisch (PSF), a well-known traditional Chinese medicine (TCM), has been used as a “heat-clearing and detoxifying” agent. The present study was to illustrate the preventive effect of PSF on pelvic inflammatory disease (PID) in rats. The PID model was constructed by multipathogen infection of the upper genital tract with reference to the method previously reported. Urine metabolomic analysis was conducted with a GC-MS coupled with derivatization method. In this study, PID rats showed obvious infiltration of inflammatory cells and elevated expression of cytokines (IL-1β and IL-6) in upper genital tract, compared with control rats. Sixteen differentiating metabolites contributed to the alteration of metabolic profile in PID rats, including two amino acids, three fat acids, nine organic acids, and two types of sugars. The rats, infected by multipathogen and administered with PSF, showed decreased infiltration of inflammatory cells and lowered expression of cytokines in upper genital tract, compared with PID rats. Meanwhile, PSF intervened in the PID-associated alterations in TCA cycle, sugar metabolism, amino acid metabolism, and other uncertain metabolic pathways. These results indicate that PSF has preventive effect on multipathogen induced PID and holistic interventional effect on disease-associated metabolomic change.

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Cytokines, estradiol and progesterone in the plasma of women of reproductive age with pelvic inflammatory disease in remission

Cited by 5 publications

References 5 publications

Anti‐Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF‐κB Pathway

Anti‐Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF‐κB Pathway

Gas chromatography-mass spectrometric method-based urine metabolomic profile of rats with pelvic inflammatory disease

Metabolomic Study on the Preventive Effect ofPatrinia scabiosaefoliaFisch on Multipathogen Induced Pelvic Inflammatory Disease in Rats

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