Cytokinesis-block micronucleus cytome assay parameters in peripheral blood lymphocytes of the general population: Contribution of age, sex, seasonal variations and lifestyle factors

Gajski, Goran; Gerić, Marko; Oreščanin, Višnja; Garaj‐Vrhovac, Vera

doi:10.1016/j.ecoenv.2017.11.003

Cited by 60 publications

(38 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The demographic and genotypic features of the investigated urban population are reported in As for the role of sex, no significant differences we observed between females and males in the frequencies of MNi, NPBs and NBUDs (Tables and 3). This result contrasts with that recently observed by Gajski et al (2018) with the same cytogenetic biomarker. A convincing explanation for a possible gender difference in genomic damage levels is still missing.…”

Section: General Characteristics Of the Studied Populationcontrasting

confidence: 99%

Micronuclei frequency in peripheral blood lymphocytes of healthy subjects living in Turin (North-Italy): contribution of body mass index, age and sex

Santovito

Gendusa

2020

Annals of Human Biology

View full text Add to dashboard Cite

Referee 1: "This study describes more general frequency of CBMN assay biomarkers and could be regarded as the study giving baseline values of MN assay parameters. Saying that this is connected with the air pollution because Turin is regarded as one of the most polluted cities in Italy is a bit overestimated. To connect MN frequency with the biomarkers of exposure to air pollution one should measure biomarkers of exposure or at least present certain data regarding air pollution in the time frame of the blood sampling to access the exact levels of air pollutants during the sapling of population. Without that, this paper is simply biomonitoring study undertaken to access baseline frequency of MN assay parameters in a healthy population of the city of Turin. In line with that authors should change the title and the aim of the study. Trough discussion section one could hypothesis that the higher MN frequency could be due to air pollution but nothing more". Authors: In agreement with the referee, we changed title and aim of the study. Introduction and Discussion sessions were modified, trying to give more emphasis to the population aspects. Moreover, as suggested by the referee 2, the introduction session was improved with some concepts about the evolutionary meaning of MNi. Referee 1: "Authors could even give some data on the air pollution parameters form their city in the year of blood sampling but without that this seems a bit overestimated to say that the higher frequency is due to air pollution since it could be from several other lifestyle factors, dietary preferences, polluted water etc". Authors: In agreement with the referee, we provide table (Table 5) with data about air pollution in Turin in 2017, year of sampling Referee 1: "Page 6, line 33-use × instead of X" Authors: In agreement with the referee, we modified the text as suggested.

show abstract

Section: General Characteristics Of the Studied Populationcontrasting

confidence: 99%

Micronuclei frequency in peripheral blood lymphocytes of healthy subjects living in Turin (North-Italy): contribution of body mass index, age and sex

Santovito

Gendusa

2020

Annals of Human Biology

View full text Add to dashboard Cite

show abstract

“…Considering this valuable information, the mean MN frequency of all participants in our study was found to be 3.91 ± 1.82‰, 3.69 ± 1.75‰ in female group, and 4.12 ± 1.87‰ in male group. Whereas those values were reported to be higher in adults such as 5.06 ± 3.11‰ in all subjects, 5.16 ± 2.98‰ in females, and 4.79 ± 3.44‰ in males by Gajski et al [15]. e major difference between those studies and our study is the age groups of the participants.…”

Section: Discussioncontrasting

confidence: 84%

“…e main factors to affect the frequency of MN were analysed, and they are confirming the age and sex as the main factors associated with the frequency. However, as pointed out by some studies, normal values for micronucleus test in healthy populations has been suggested by some authors due to the wide range of results of this test [14,15,17]. Considering this valuable information, the mean MN frequency of all participants in our study was found to be 3.91 ± 1.82‰, 3.69 ± 1.75‰ in female group, and 4.12 ± 1.87‰ in male group.…”

Section: Discussionsupporting

confidence: 57%

“…is variability has been linked to study designs, different experimental conditions, and population characteristics such as genetic and lifestyle factors [15]. In the light of those observations and reports, requirement of normal reference values has been pointed out by some authors [14,15]. In this study we aimed to present the age-specific MN values in 150 apparently healthy children aged 1 to 15.…”

Section: Introductionmentioning

confidence: 97%

“…Although this test has been reported to be reliable and useful, large variations are observed in the literature [13,14]. is variability has been linked to study designs, different experimental conditions, and population characteristics such as genetic and lifestyle factors [15]. In the light of those observations and reports, requirement of normal reference values has been pointed out by some authors [14,15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Lymphocyte Cytokinesis-Block Micronucleus Values in Apparently Healthy Children by means of Age and Sex

Durmaz

Taslidere

Koturoğlu

et al. 2019

BioMed Research International

View full text Add to dashboard Cite

The cytokinesis-block micronucleus (MN) assay on blood lymphocytes is one of the most important tests implemented in cytogenetics for the measurement of genotoxicity. For the purpose of biological dosing, it is crucial to know the spontaneous frequency of MN and its normal values in general population, especially in children, which are used for the population databases. In this study, MN levels were investigated in cytokinesis-blocked lymphocytes of 150 apparently healthy children aged 1 to 15. Our aim was to assess the variability of MN values according to age and sex. The mean MN frequency among boys was 3.69 ± 1.747‰ and 4.12 ± 1.867‰ in girls where there was no significant difference in relation to age and sex. However, when we separated age groups as 0–2 years, 3–5 years, 6–10 years, and 11–15 years, one-way ANOVA test showed significant association. Significance was obvious in the 0–2 years age group with the 3–5 years age group and 6–10 years age group. When we grouped our study population as 0–2 years and 3–15 years, the mean MN frequency among the 0–2 years age group was 2.85 ± 1.599‰ and 4.07 ± 1.867‰ in the 3–15 years age group which was also statistically significant. This difference may be attributed to age-related increase of close contact with environmental hazardous agents. In conclusion, normal values of MN obtained in this study will add valuable information in regard to update the current childhood population data and will act as a reference for further genotoxicity studies.

show abstract

Genotoxic Activity of Secondary Metabolites of Teucrium Species

Grujičić

Marinković

Milošević-Djordjević

2020

Teucrium Species: Biology and Applications

View full text Add to dashboard Cite

Cytokinesis-block micronucleus cytome assay parameters in peripheral blood lymphocytes of the general population: Contribution of age, sex, seasonal variations and lifestyle factors

Cited by 60 publications

References 72 publications

Micronuclei frequency in peripheral blood lymphocytes of healthy subjects living in Turin (North-Italy): contribution of body mass index, age and sex

Micronuclei frequency in peripheral blood lymphocytes of healthy subjects living in Turin (North-Italy): contribution of body mass index, age and sex

Determination of Lymphocyte Cytokinesis-Block Micronucleus Values in Apparently Healthy Children by means of Age and Sex

Genotoxic Activity of Secondary Metabolites of Teucrium Species

Contact Info

Product

Resources

About