Cytokinesis-block micronucleus cytome (CBMN-CYT) assay biomarkers and telomere length analysis in relation to inorganic elements in individuals exposed to welding fumes

Quintana-Sosa, Milton; León-Mejía, Grethel; Luna-Carrascal, Jaime; Moya, Yurina Sh De; Rodríguez, Ibeth Luna; Acosta-Hoyos, Antonio; Anaya-Romero, Marco; Trindade, Cristiano; Narváez, Diana; Restrepo, Helena Groot de; Dias, Johnny Ferraz; Niekraszewicz, Liana Appel Boufleur; Garcia, Ana Letícia Hilário; Rohr, Paula; Silva, Juliana da; Henriques, João Antônio Pêgas

doi:10.1016/j.ecoenv.2021.111935

Cited by 16 publications

(3 citation statements)

References 64 publications

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“…When DNA damage is tolerable, it can lead to different responses, such as cell cycle arrest and DNA repair; however, when the damage is excessive or irreparable, it can lead to cell death to counteract the damage caused by carcinogens (Jarvis et al 2014;Matt and Hofmann 2016). Regarding the NDI biomarker, which measures cytostasis and the proliferative state of the viable cell fraction, no significant differences were observed between the exposed and control groups (León-Mejía et al 2019;Quintana-Sosa et al 2021).…”

Section: Discussionmentioning

confidence: 96%

Analysis of the cytotoxic and genotoxic effects in a population chronically exposed to coal mining residues

León-Mejía

Rueda

Pérez

et al. 2023

Environ Sci Pollut Res

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During coal mining activities, many compounds are released into the environment that can negatively impact human health. Particulate matter, polycyclic aromatic hydrocarbons (PAHs), metals, and oxides are part of the complex mixture that can affect nearby populations. Therefore, we designed this study to evaluate the potential cytotoxic and genotoxic effects in individuals chronically exposed to coal residues from peripheral blood lymphocytes and buccal cells. We recruited 150 individuals who lived more than 20 years in La Loma-Colombia and 120 control individuals from the city of Barranquilla without a history of exposure to coal mining. In the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay, significant differences in the frequency of micronucleus (MN), nucleoplasmic bridge (NPB), nuclear bud (NBUD), and apoptotic cells (APOP) were observed between the two groups. In the buccal micronucleus cytome (BM-Cyt) assay, a significant formation of NBUD, karyorrhexis (KRX), karyolysis (KRL), condensed chromatin (CC), and binucleated (BN) cells was observed in the exposed group. Considering the characteristics of the study group, a significant correlation for CBMN-Cyt was found between NBUD and vitamin consumption, between MN or APOP and meat consumption, and between MN and age. Moreover, a significant correlation for BM-Cyt was found between KRL and vitamin consumption or age, and BN versus alcohol consumption. Using Raman spectroscopy, a significant increase in the concentration of DNA/RNA bases, creatinine, polysaccharides, and fatty acids was detected in the urine of individuals exposed to coal mining compared to the control group. These results contribute to the discussion on the effects of coal mining on nearby populations and the development of diseases due to chronic exposure to these residues.

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Section: Discussionmentioning

confidence: 96%

Analysis of the cytotoxic and genotoxic effects in a population chronically exposed to coal mining residues

León-Mejía

Rueda

Pérez

et al. 2023

Environ Sci Pollut Res

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“…Previous studies by León-Mejía et al, ( 2023a , 2023b ) reported significant concentrations of S and Fe in the blood of the exposed population. These elements, present in the inhaled particles, might be absorbed by various tissues and subsequently eliminated through urine (Quintana-Sosa et al, 2021 ), suggesting a systemic effect on these individuals. Metallic elements can directly interact with organic molecules or indirectly generate ROS, resulting in DNA damage and structural changes in cellular components, thus contributing to the development of various diseases (Chen et al, 2018 ; Jomova & Valko, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

The dangerous link between coal dust exposure and DNA damage: unraveling the role of some of the chemical agents and oxidative stress

Miranda-Guevara,

Muñoz-Acevedo,

Fiorillo-Moreno

et al. 2023

Environ Geochem Health

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Exposure to coal mining dust poses a substantial health hazard to individuals due to the complex mixture of components released during the extraction process. This study aimed to assess the oxidative potential of residual coal mining dust on human lymphocyte DNA and telomeres and to perform a chemical characterization of coal dust and urine samples. The study included 150 individuals exposed to coal dust for over ten years, along with 120 control individuals. The results revealed significantly higher levels of DNA damage in the exposed group, as indicated by the standard comet assay, and oxidative damage, as determined by the FPG-modified comet assay. Moreover, the exposed individuals exhibited significantly shorter telomeres compared to the control group, and a significant correlation was found between telomere length and oxidative DNA damage. Using the PIXE method on urine samples, significantly higher concentrations of sodium (Na), phosphorus (P), sulfur (S), chlorine (Cl), potassium (K), iron (Fe), zinc (Zn), and bromine (Br) were observed in the exposed group compared to the control group. Furthermore, men showed shorter telomeres, greater DNA damage, and higher concentrations of nickel (Ni), calcium (Ca), and chromium (Cr) compared to exposed women. Additionally, the study characterized the particles released into the environment through GC–MS analysis, identifying several compounds, including polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene, naphthalene, anthracene, 7H-benzo[c]fluorene, phenanthrene, pyrene, benz[a]anthracene, chrysene, and some alkyl derivatives. These findings underscore the significant health risks associated with exposure to coal mining dust, emphasizing the importance of further research and the implementation of regulatory measures to safeguard the health of individuals in affected populations. Graphic abstract

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“…can lead to cell death to counteract the damage caused by carcinogens(Jarvis et al, 2014;Matt and Hofmann, 2016). Regarding the NDI biomarker, which measures cytostasis and the proliferative state of the viable cell fraction, no signi cant differences were observed between the exposed and nonexposed groups(León-Mejía et al, 2019;Quintana-Sosa et al, 2021).…”

mentioning

confidence: 99%

Analysis of the Cytotoxic and Genotoxic Effects in a Population Chronically Exposed to Coal-Mining Residues

León-Mejía¹,

Rueda

Pérez

et al. 2022

Preprint

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During coal-mining activities, many compounds are released into the environment that can negatively impact human health. Particulate matter, polycyclic aromatic hydrocarbons (PAHs), metals, and oxides are part of the complex mixture that can affect nearby populations. Therefore, this study was designed to evaluate the potential cytotoxic and genotoxic effects of coal residues on peripheral blood lymphocytes and buccal cells in individuals chronically exposed to them. We recruited 150 individuals who lived more than 20 years in the Loma-Colombia and 120 control individuals from the city of Barranquilla without a history of exposure to coal-mining. In cytokinesis-block micronucleus cytome (CBMN-Cyt) assay, signi cant differences in the frequency of micronucleus (MN), nucleoplasmic bridge (NPB), nuclear bud (NBUD), and apoptotic cells (APOP) were observed between the two groups. In buccal micronucleus cytome (BM-Cyt) assay, a signi cant formation of NBUD, karyorrhexis (KRX), karyolysis (KRL), condensed chromatin (CC), and binucleated (BN) cells was observed in the exposed group. Considering sociodemographic factors, a signi cant correlation was found between CBMN-Cyt-NBUD and vitamin consumption and between CBMN-Cyt-MN and CBMN-Cyt-APOP and meat consumption. Moreover, a signi cant correlation was found between BM-Cyt-KRL and vitamin consumption. Using Raman spectroscopy, a signi cant increase in the concentration of DNA/RNA bases, creatinine, polysaccharides and fatty acids was detected in the urine of individuals exposed to coal-mining compared to the control group. These results contribute to the discussion on the effects of coal-mining on nearby populations and the development of diseases due to chronic exposure to these residues.

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Cytokinesis-block micronucleus cytome (CBMN-CYT) assay biomarkers and telomere length analysis in relation to inorganic elements in individuals exposed to welding fumes

Cited by 16 publications

References 64 publications

Analysis of the cytotoxic and genotoxic effects in a population chronically exposed to coal mining residues

Analysis of the cytotoxic and genotoxic effects in a population chronically exposed to coal mining residues

The dangerous link between coal dust exposure and DNA damage: unraveling the role of some of the chemical agents and oxidative stress

Analysis of the Cytotoxic and Genotoxic Effects in a Population Chronically Exposed to Coal-Mining Residues

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