Cytokinesis-blocked micronucleus cytome and comet assays in peripheral blood lymphocytes of workers exposed to lead considering folate and vitamin B12 status

Minozzo, Renato; Deimling, Luiz Irineu; Santos-Mello, Renato

doi:10.1016/j.mrgentox.2010.01.009

Cited by 27 publications

(20 citation statements)

References 99 publications

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“…Similarly, significant differences were also found for the frequency of NBUDs in exposed subjects in Tuzla. Our results are in strong correlation with the results suggesting that the frequencies of NPBs and NBUDs were significantly higher in the Pb‐exposed group as compared to the values measured in the control groups (Minozzo et al, ). Similarly, Rohr et al () demonstrated that the groups exposed to coal had significantly increased frequency of NPBs compared with unexposed individuals.…”

Section: Discussionsupporting

confidence: 91%

Assessment of the genotoxicity and cytotoxicity in environmentally exposed human populations to heavy metals using the cytokinesis‐block micronucleus cytome assay

Mesic

Nefic

2014

Environmental Toxicology

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The cytokinesis-block micronucleus cytome (CBMN-Cyt) assay was developed as a system for evaluating DNA damage, cytostasis, and cytotoxicity. The aim of the present study was to estimate levels of micronuclei (MNi), nucleoplasmic bridges (NPBs), nuclear buds (NBUDs), cell death (apoptosis/necrosis), nuclear division index, and nuclear division cytotoxicity index values in the peripheral blood lymphocytes of environmentally exposed subjects to heavy metals from five Bosnian regions, characterized by different exposure to heavy metals. The study was performed using CBMN-Cyt assay, considering factors, such as age, gender and smoking habits and their possible effects on analyzed parameters. In total, 104 healthy subjects were selected (49.04% females and 50.96% males; average age, 35.41 years; 51.92% smokers and 48.08% nonsmokers). There was significant difference between the frequency of NBUDs in Tuzla as compared to the control group. Furthermore, there was observed a statistically significant difference for the frequency of NPBs between Zenica, Olovo, and Kakanj when compared with the controls. Males showed a significantly higher number of apoptotic cells than females in controls. There were significant differences between smokers and nonsmokers in the frequency of NPBs in controls (higher in nonsmokers) and necrotic cells in Olovo (higher in nonsmokers). The pack years of smoking significantly influenced the number of necrotic cells in controls and the frequency of NBUDs in the overall sample. The results of the present study provide evidence of significantly increased frequency of NPBs and NBUDs in exposed subjects, suggesting that these endpoints are highly sensitive markers for measuring genotoxicity.

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Section: Discussionsupporting

confidence: 91%

Assessment of the genotoxicity and cytotoxicity in environmentally exposed human populations to heavy metals using the cytokinesis‐block micronucleus cytome assay

Mesic

Nefic

2014

Environmental Toxicology

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“…A positive correlation for both nonexposed subjects and welders was observed between MN rate and age ( Figure 3B); namely, the higher the detected MN rate, the higher the age. It was reported that there was a positive correlation between MN and age (18).…”

Section: Discussionmentioning

confidence: 96%

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal-arc welding

Şener¹,

Eroğlu

2013

Turk J Med Sci

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To foster the practical development of the constructed wetlands used for water quality enhancement in Turkey, 2 vertical subsurface flow pilot-scale constructed wetlands were implemented on the METU campus, Ankara, Turkey. Both of the wetlands were planted with Phragmites australis and operated identically at a flowrate of 3 m 3 .d −1 and a hydraulic loading rate (HLR) of 0.100 m.d −1 , intermittently. The main objective of the research was to quantify the effect of different substrates (gravel and blast furnace granulated slag) on the nutrient removal performance of the constructed wetlands in the prevailing climate of Ankara. According to the monitoring study (July 2002-January 2003), concentration based average removal efficiencies for the slag and gravel reed beds were as follows: TSS (64% and 62%), COD (49% and 40%), NH + 4 -N (88% and 58%), TN (41% and 44%), TP (63% and 9%) and PO 3− 4 -P (60% and 4%). In general, the treatment performance of the slag system was better than that of the gravel system.

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“…Studies assessing folate and other vitamins levels in plasma did not find a protective effect either: even though there were great differences in folate levels among populations exposed to air-pollution, no protective effect from oxidative DNA damage such as M1dG and 8-oxodG adducts was found by Singh et al [2007]. A study with lead-exposed workers, reported higher MN frequencies in the subgroup showing higher-than-the-mean serum folate levels [Minozzo et al, 2010]; these workers were assumed to consume folate-enriched wheat and maize flour in food. According to these results, whether folic acid supplementation is enough to protect against environmental pollutant-induced DNA-damage needs to be further explored.…”

Section: Folic Acid Intakementioning

confidence: 95%

Assessment of exposure to organochlorine pesticides and levels of DNA damage in mother–infant pairs of an agrarian community

Alvarado-Hernández

Montero‐Montoya

Serrano-García

et al. 2013

Environ and Mol Mutagen

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Exposure to organochlorine pesticides was studied in a group of mother-infant pairs living in a rural area where agriculture is the main economic activity. Fumigation in this zone is performed with airplanes, thus affecting the inhabited areas around them, including schools. Heparinized venous blood of mothers and umbilical cords was used to evaluate the olive tail moment in the comet assay, and micronuclei, chromatin buds, and nucleoplasmic bridges in peripheral blood lymphocytes. Cord blood samples were taken at the moment of birth only from natural and normal parturitions. Determinations of hexachlorobenzene, aldrin, heptachlor epoxide, oxichlordane, t and c-chlordane, cis-nonachlor, mirex, alpha and beta-endosulfan, alpha, beta and gamma hexachlorocyclohexane, and p'p'-DDT, p'p'-DDE were conducted to establish the differential distribution of the toxicants between compartments, i.e., mother and umbilical cord. Significantly higher pesticide levels were found in umbilical cord plasma than in mothers' plasma for almost all compounds tested, except DDE and oxychlordane. Significantly higher olive tail moments were found in umbilical cords than in mothers, whereas micronuclei frequencies were higher in mothers than in umbilical cords. However, neither the levels of micronuclei nor the olive tail moment were correlated with pesticide levels. Given that no other exposure to toxic compounds has been identified in this region, the lack of correlation between genotoxicity biomarkers and pesticide levels may be due to the variability of the exposure and to endogenous processes related to lipid mobility during pregnancy, the metabolism of the compounds, and individual susceptibilities.

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Cytokinesis-blocked micronucleus cytome and comet assays in peripheral blood lymphocytes of workers exposed to lead considering folate and vitamin B12 status

Cited by 27 publications

References 99 publications

Assessment of the genotoxicity and cytotoxicity in environmentally exposed human populations to heavy metals using the cytokinesis‐block micronucleus cytome assay

Assessment of the genotoxicity and cytotoxicity in environmentally exposed human populations to heavy metals using the cytokinesis‐block micronucleus cytome assay

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal-arc welding

Assessment of exposure to organochlorine pesticides and levels of DNA damage in mother–infant pairs of an agrarian community

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