Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint

Mulvihill, Daniel P.; Hyams, Jeremy S.

doi:10.1242/jcs.00031

Cited by 34 publications

(26 citation statements)

References 68 publications

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“…3C and supplementary material Movie 2). The rate of CAR constriction in these cells is equivalent to that reported previously, providing further evidence of GFP-Cdc8 functionality (Bezanilla et al, 2000;Kitayama et al, 1997;Mulvihill and Hyams, 2002).…”

Section: Cdc8 Localises To Actin Filaments During Interphasesupporting

confidence: 85%

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Acetylation regulates tropomyosin function in the fission yeastSchizosaccharomyces pombe

Skoumpla

Coulton

Lehman

et al. 2007

Journal of Cell Science

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121

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Tropomyosin is an evolutionarily conserved α-helical coiled-coil protein that promotes and maintains actin filaments. In yeast, Tropomyosin-stabilised filaments are used by molecular motors to transport cargoes or to generate motile forces by altering the dynamics of filament growth and shrinkage. The Schizosaccharomyces pombe tropomyosin Cdc8 localises to the cytokinetic actomyosin ring during mitosis and is absolutely required for its formation and function. We show that Cdc8 associates with actin filaments throughout the cell cycle and is subjected to post-translational modification that does not vary with cell cycle progression. At any given point in the cell cycle 80% of Cdc8 molecules are acetylated, which significantly enhances their affinity for actin. Reconstructions of electron microscopic images of actin-Cdc8 filaments establish that the majority of Cdc8 strands sit in the `closed' position on actin filaments, suggesting a role in the regulation of myosin binding. We show that Cdc8 regulates the equilibrium binding of myosin to actin without affecting the rate of myosin binding. Unacetylated Cdc8 isoforms bind actin, but have a reduced ability to regulate myosin binding to actin. We conclude that although acetylation of Cdc8 is not essential, it provides a regulatory mechanism for modulating actin filament integrity and myosin function.

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Section: Cdc8 Localises To Actin Filaments During Interphasesupporting

confidence: 85%

“…Cells were grown in minimal medium (EMM2) supplemented with the appropriate amino acids. Synchronous cultures were generated by transient arrest using the cdc25-22 mutation in a strain possessing the myo2-gfp allele (Mulvihill and Hyams, 2002).…”

Section: Yeast Cell Culture and Strainsmentioning

confidence: 99%

Acetylation regulates tropomyosin function in the fission yeastSchizosaccharomyces pombe

Skoumpla

Coulton

Lehman

et al. 2007

Journal of Cell Science

Self Cite

121

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“…Mitotic cells treated with a high dose of MBC typically result in the lethal 'cut' phenotype, which displays enforced cytokinesis with unsegregated chromosomes [14][15][16] . To determine whether the ipMT independent division observed during MII might result in abnormal segregation of chromosomes, we labelled the centromeres of chro mosome II (cen2) with GFP (the cen2GFP system 17 ) and filmed cells to make kymographs.…”

Section: Normal Chromosome Segregation In Absence Of Ipmts In MIImentioning

confidence: 99%

“…SAC components including Mad2 recognize unattached kinetochores and inhibit the activation of the anaphasepromoting complex/cyclosome so that cells can wait at metaphase until the attachment improves 19 . During mitosis, MBC causes spindle defects and activates SAC 15,20 . It is possible that SAC activity is required for the ipMTindependent MII caused by MBC.…”

Section: Normal Chromosome Segregation In Absence Of Ipmts In MIImentioning

confidence: 99%

Interpolar microtubules are dispensable in fission yeast meiosis II

2012

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The mitotic spindle consists of two types of microtubules. Dynamic kinetochore microtubules capture kinetochores, whereas stable interpolar microtubules serve as the structural backbone that connects the two spindle poles. Both have been believed to be indispensable for cell division in eukaryotes. Here we demonstrate that interpolar microtubules are dispensable for the second division of meiosis in fission yeast. Even when interpolar microtubules are disrupted by a microtubule-depolymerizing drug, spindle poles separate and chromosomes segregate poleward in second division of meiosis in most zygotes, producing viable spores. The forespore membrane, which encapsulates the nucleus in second division of meiosis and is guided by septins and the leading-edge proteins, is responsible for carrying out meiotic events in the absence of interpolar microtubules. Furthermore, during physiological second division of meiosis without microtubule perturbation, the forespore membrane assembly contributes structurally to spindle pole separation and nuclear division, generating sufficient force for spindle pole separation and subsequent events independently of interpolar microtubules.

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“…The mitotic arrest deficiency (Mad) and budding uninhibited by benomyl (Bub) proteins are key components of the mitotic spindle checkpoint conserved in organisms ranging from yeast to humans. The conserved components of the mitotic spindle checkpoint act at various stages of mitosis to coordinate spindle assembly and sister-chromatid separation as well as to delay mitotic exit and cytokinesis in response to spindle defects (Booher and Beach 1988;Mulvihill and Hyams 2002;Musacchio and Salmon 2007). However, the existence of surveillance mechanisms capable of verifying microtubule integrity prior to the commitment to mitosis is controversial (Uetake and Sluder 2007).…”

mentioning

confidence: 99%

A G2-Phase Microtubule-Damage Response in Fission Yeast

Balestra

Jiménez

2008

Genetics

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Microtubules assume a variety of structures throughout the different stages of the cell cycle. Ensuring the correct assembly of such structures is essential to guarantee cell division. During mitosis, it is well established that the spindle assembly checkpoint monitors the correct attachment of sister chromatids to the mitotic spindle. However, the role that microtubule cytoskeleton integrity plays for cell-cycle progression during interphase is uncertain. Here we describe the existence of a mechanism, independent of the mitotic checkpoint, that delays entry into mitosis in response to G 2 -phase microtubule damage. Disassembly of the G 2 -phase microtubule array leads to the stabilization of the universal mitotic inhibitor Wee1, thus actively delaying entry into mitosis via inhibitory Cdc2 Tyr15 phosphorylation.

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Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint

Cited by 34 publications

References 68 publications

Acetylation regulates tropomyosin function in the fission yeastSchizosaccharomyces pombe

Acetylation regulates tropomyosin function in the fission yeastSchizosaccharomyces pombe

Interpolar microtubules are dispensable in fission yeast meiosis II

A G2-Phase Microtubule-Damage Response in Fission Yeast

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