Cytokinin activity ofN⁶-benzyladenine derivatives assayed by interaction with the receptorsin planta, in vitro, andin silico

Abstract

Individual cytokinin receptors from Arabidopsis were assayed in planta, in vitro and in silico with 25 different 6-benzyladenine derivatives, new receptor-specific cytokinins were revealed.not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission.The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/241281 doi: bioRxiv preprint first posted online Jan. The copyright holder for this preprint (which was . http://dx.doi.org/10.1101/241281 doi: bioRxiv preprint first posted online Jan. and its ability to activate the same receptor in planta were evaluated for each AHK protein.Interaction patterns between individual receptors and ligands were rationalized by structure analysis and molecular docking in sensory modules of AHK receptors. The best correlation between docking scores and specific binding was observed for AHK3. In addition, receptorspecific ligands have been discovered with unique properties to predominantly activate or block distinct cytokinin receptors. These ligands are promising for practical application and as molecular tools in the study of the cytokinin perception by plant cells.not peer-reviewed)