Cytokinin delays dark-induced senescence in rice by maintaining the chlorophyll cycle and photosynthetic complexes

Talla, Sai Krishna; Panigrahy, Madhusmita; Kappara, Saivishnupriya; Nirosha, P; Neelamraju, Sarla; Ramanan, Rajeshwari

doi:10.1093/jxb/erv575

Cited by 90 publications

(61 citation statements)

References 56 publications

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“…Nagina 22 is a well-known aus variety used routinely as a check for heat tolerance in rice (Jagadish et al, 2010). In a network project, EMS-induced stabilized N22 mutants were evaluated for different traits, such as tolerance to herbicide, heat, low P, water deficiency, and salinity and resistance to bacterial leaf blight and blast (Kulkarni et al, 2013(Kulkarni et al, , 2014Lima et al, 2015;Mithra et al, 2016;Mohapatra et al, 2014;Panigrahy et al, 2009Panigrahy et al, , 2011Panigrahy et al, , 2014Poli et al, 2013;Talla et al, 2016). As a part of the network project on functional genomics, a total of 2500 N22 mutants were screened at the Indian Institute of Rice Research (IIRR) under field conditions maintained with low P (Olsen P, <1.8) to identify low-P-tolerant and susceptible mutants.…”

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confidence: 99%

Comparing Hydroponics, Sand, and Soil Medium To Evaluate Contrasting Rice Nagina 22 Mutants for Tolerance to Phosphorus Deficiency

et al. 2017

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Soils deficient in P are widespread in major rice (Oryza sativa L.) ecosystems. In view of declining reserves of rock phosphate and the rising costs of P fertilizers, breeding rice varieties tolerant to low P has become important for future food security. Four different methods (hydroponics without sand [H], hydroponics with sand [HS], large pots with soil [PS], and glasses with soil [GS]) were evaluated using rice aus variety Nagina 22 (N22) and its known gain‐of‐function (gof) and loss‐of‐function (lof) mutants to screen for low P tolerance in the field. In −P (no addition of NaH2PO4) shoots, dry weight was significantly higher in gof mutant NH787 than in N22 in HS, PS, and GS but not in H, with fold increases of 1.8 in HS, 5.2 in GS, and 9.4 in PS. In HS, in −P, out of six traits only shoot dry weight was significantly higher in gof and lower in lof mutants. However, in GS both root and shoot dry weight could confirm gof and lof mutants. It took 40 d in GS and 70 d in PS to differentiate between growth in –P/low P and +P (0.32 mM NaH2PO4) and between gof and lof mutants. Thus, shoot dry weight at 30 d in HS and root and shoot dry weight at 40 d in GS are best to differentiate between genotypes grown in –P/low P and +P soil and also between gof and lof mutants for low P tolerance. The HS method can be performed in ambient conditions and needs 70% less medium compared with H. If germplasm is to be screened for low P tolerance on a large scale and if there is no access to low‐P soil, then screening using HS is the best approach.

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confidence: 99%

Comparing Hydroponics, Sand, and Soil Medium To Evaluate Contrasting Rice Nagina 22 Mutants for Tolerance to Phosphorus Deficiency

et al. 2017

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“…7B). External applications of CK has also been shown to improve chlorophyll retention and delay dark-induced senescence in leaves (Gan and Amasino 1995; Kim et al 2006; Talla et al 2016). As well, increased endogenous levels of CKs have been shown to be able to delay senescence in plants (Richmond and Lang, 1957; Lin et al, 2002; Ma and Liu, 2009; Zhang et al, 2010; Liu et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…Significant progress have been made in our understanding of the role of cytokinins (CK) in plant growth, and development. Through interactions with other hormonal pathways and transcriptional factors, CKs plays an important role in a variety of plant processes including greening (Cortleven and Schmülling, 2015), etioplast-to-chloroplast transition (Cortleven et al, 2016), root greening (Kobayashi et al, 2012; 2017), shoot and shoot development (Howell et al, 2003; Kieber and Schaller, 2014; Raines et al, 2016) and delay in leaf senescence (Gan and Amasino, 1995; Kim et al, 2006; Kieber and Schaller, 2014; Raines et al, 2016; Talla et al, 2016). The current knowledge of CK signalling involves a multistep signal transduction system that begins with the perception of CK by the Arabidopsis Histidine Kinases ( AHK2 , AHK3 , CRE1 / AHK4 ) in the endoplasmic reticulum (ER) phosphate transfer via the Arabidopsis Histidine Phosphotransfer proteins (AHPs).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis geneRGOmediates cytokinin responses and increases seed yield

Murmu

Allard

Chabot

et al. 2020

Preprint

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17One-sentence summary: RGO, a novel gene from Arabidopsis, is essential for plant development, 18 mediates CK signaling and increases seed yield in Arabidopsis and rapeseed when overexpressed. Abstract 1 A novel gene, At1g77960, from Arabidopsis thaliana was characterized. At1g77960 transcripts 2 accumulate to very high levels in plants ectopically overexpressing the Golden2-like1 (GLK1) 3 transcription factor and is designated as a Response to GLK1 Overexpression (RGO) gene. RGO 4 encodes a protein with domains of tandem QH and QN repeats. Transcripts and promoter GUS 5 reporter analyses indicated that RGO is expressed in roots, leaves, stems, floral and siliques tissues 6 but not in seeds. Expression of the RGO:YFP fusion protein demonstrated that RGO is localized 7 to the endoplasmic reticulum. MicroRNA mediated silencing of RGO resulted in severe reductions 8 in vegetative and root growth, delayed flowering and reduced seed yield and viability, suggesting 9 that RGO is essential for plant development. Conversely, ectopic overexpression of RGO resulted 10 in enhanced vegetative growth including increased axillary bud formation and a 20% higher seed 11 yield. Stable overexpression of RGO in Brassica napus also produced a similar increase in seed 12 yield. Cytokinin (CK) response assays including root growth, green calli formation from excised 13 hypocotyls and chlorophyll retention during dark-induced senescence suggest that one role of RGO 14 is to mediate CK responses in plant development. These results suggest that RGO could be a target 15 gene for increasing crop seed yields. 16 Keywords 17 Arabidopsis thaliana, At1g77960, GLK1 overexpression, cytokinin response, seed yield 18 19 [Type text] [Type text] 4 1A direct link between GLKs and CK signalling however, is lacking. There is evidence to suggest 2 that type B ARRs coordinate the expression of HY5 (elongated hypocotyl 5), which is required by 3 GLK2 for maximal (although not essential) greening in roots (Kobayashi et al., 2012).

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“…It is known that endogenous factors such as plant growth regulators, reproduction and cellular differentiation also influence senescence. Among these endogenous factors, various plant hormones such as auxin, cytokinin, ethylene and jasmonic acid etc., have been characterized most comprehensively at the molecular and physiological level [17,21,32,35,39]. More recently, the roles of an additional plant hormone strigolactones (SLs) were intensely discussed during leaf senescence development [41].…”

Section: Introductionmentioning

confidence: 99%

Response to Plant Hormones of Senescence-related Genes for Cucumis sativus L. in Cotyledon Development

Jeong¹,

Kim²

2016

Journal of Life Science

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This study was carried out to discover the response of cucumber (Cucumis sativus L.) senescence-associated genes (SAGs) to several plant hormones in detached and developing cotyledon. Accordingly, a collection of cucumber SAGs were examined to characterize their gene expression response through semi-quantitative RT-PCR. Cotyledons were excised at day 14 after seed sowing from plantlets, then incubated in 100 μM each of IAA or zeatin solution for up to 4 days in light and darkness. They were collected at 2-day intervals and used for total RNA extraction and subjected to RT-PCR. Gene expression levels of several cucumber SAGs were significantly changed during the incubation period. More than five cucumber SAGs involving SAG 60 responded to the IAA and zeatin treatment. In the ethylene response study, cotyledons were exposed up to 10 days by ethylene gas. Most of the cucumber SAGs did not show immediate response to ethylene in green cotyledon. The exceptions were PCK, SAG 158, and SAG 288 genes, which responded after 1 day of exposure to green cotyledon, while ICL and SAG 281 revealed strong responses after 10 days of being exposed to yellowing cotyledon. These results suggest that several cucumber SAGs react actively in response to starvation or senescence against exogenously applied stimulus. This induced senescence response is able to understand the SAGs role in lipids and amino acids metabolism partly and function in organ senescence during development.

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Cytokinin delays dark-induced senescence in rice by maintaining the chlorophyll cycle and photosynthetic complexes

Abstract: HighlightCytokinin induces functional stay-greenness by maintaining the Chl a/b ratios and the stability of photosynthetic complexes during dark-induced senescence in rice.

Cited by 90 publications

References 56 publications

Comparing Hydroponics, Sand, and Soil Medium To Evaluate Contrasting Rice Nagina 22 Mutants for Tolerance to Phosphorus Deficiency

Comparing Hydroponics, Sand, and Soil Medium To Evaluate Contrasting Rice Nagina 22 Mutants for Tolerance to Phosphorus Deficiency

The Arabidopsis geneRGOmediates cytokinin responses and increases seed yield

Response to Plant Hormones of Senescence-related Genes for Cucumis sativus L. in Cotyledon Development

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