Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry

Novák, Ondřej; Hauserová, Eva; Amakorová, Petra; Doležal, Karel; Strnad, Miroslav

doi:10.1016/j.phytochem.2008.04.022

Cited by 216 publications

(203 citation statements)

References 38 publications

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“…Deuterium-labeled cytokinin internal standards (Olchemim Ltd., Czech Republic) were added, each at 1 pmol per sample, to assess the recovery during purification and to validate the determination (44). The samples were purified with a combined cation (SCX cartridge) and anion (DEAE-Sephadex C18 cartridge) exchanger and immunoaffinity chromatography based on wide range-specific monoclonal antibodies against cytokinins (45).…”

Section: Plasmid Construction and Arabidopsis Transformation-mentioning

confidence: 99%

“…Quantification was conducted by the multiple reaction monitoring of [M ϩ H] ϩ and the appropriate product ion. For the selective multiple reaction monitoring experiments, the optimal conditions, dwell time, cone voltage, and collision energy in the collision cell corresponding to exact diagnostic transition were optimized for each cytokinin (44). Quantification was conducted using Masslynx software using a standard isotope dilution method.…”

Section: Plasmid Construction and Arabidopsis Transformation-mentioning

confidence: 99%

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A Subset of Cytokinin Two-component Signaling System Plays a Role in Cold Temperature Stress Response in Arabidopsis

Jeon

Kim

et al. 2010

Journal of Biological Chemistry

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316

288

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A multistep two-component signaling system is established as a key element of cytokinin signaling in Arabidopsis. Here, we provide evidence for a function of the two-component signaling system in cold stress response in Arabidopsis. Cold significantly induced the expression of a subset of A-type ARR genes and of GUS in Pro ARR7 :GUS transgenic Arabidopsis. AHK2 and AHK3 were found to be primarily involved in mediating cold to express A-type ARRs despite cytokinin deficiency. Cold neither significantly induced AHK2 and AHK3 expression nor altered the cytokinin contents of wild type within the 4 h during which the A-type ARR genes exhibited peak expression in response to cold, indicating that cold might induce ARR expression via the AHK2 and AHK3 proteins without alterations in cytokinin levels. The ahk2 ahk3 and ahk3 ahk4 mutants exhibited enhanced freezing tolerance compared with wild type. These ahk double mutants acclimated as efficiently to cold as did wild type. The overexpression of the cold-inducible ARR7 in Arabidopsis resulted in a hypersensitivity response to freezing temperatures under coldacclimated conditions. The expression of C-repeat/dehydration-responsive element target genes was not affected by ARR7 overexpression as well as in ahk double mutants. By contrast, the arr7 mutants showed increased freezing tolerance. The ahk2 ahk3 and arr7 mutants showed hypersensitive response to abscisic acid (ABA) for germination, whereas ARR7 overexpression lines exhibited insensitive response to ABA. These results suggest that AHK2 and AHK3 and the cold-inducible A-type ARRs play a negative regulatory role in cold stress signaling via inhibition of ABA response, occurring independently of the cold acclimation pathway.Cytokinins are plant hormones that regulate a variety of developmental and physiological processes, including cell division, cell proliferation, root and leaf differentiation, chloroplast biogenesis, and the inhibition of leaf senescence (1). Arabidopsis cytokinin signaling utilizes a multistep phospho-relay composed of a sensor kinase, a histidine phosphotransfer protein, and a response regulator similar to the TCS 2 of bacterial and yeast cells (2). A hybrid-type histidine kinase referred to as CYTOKININ INDEPENDENT1 (CKI1) is essential for megagametogenesis (3). CYTOKININ RESPONSE1 (CRE1)/ WOODEN LEG1 (WOL1)/ARABIDOPSIS HISTIDINE KINASE4 (AHK4) were shown to bind directly to a variety of natural and synthetic cytokinins in vitro with high specificity as well as in a yeast system and thus to be a primary receptor for cytokinins (4 -8). The experiments conducted using a heterologous phospho-relay system demonstrated that AHK2 and AHK3 are also cytokinin receptors. The primary functions of these Arabidopsis histidine kinase (AHK) genes involve the triggering of cell division and the maintenance of the meristematic competence of cells to prevent subsequent differentiation (9, 10). Partially redundant functions of cytokinin receptors have also been revealed in shoot growth, root development, leaf sen...

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Section: Plasmid Construction and Arabidopsis Transformation-mentioning

confidence: 99%

Section: Plasmid Construction and Arabidopsis Transformation-mentioning

confidence: 99%

A Subset of Cytokinin Two-component Signaling System Plays a Role in Cold Temperature Stress Response in Arabidopsis

Jeon

Kim

et al. 2010

Journal of Biological Chemistry

Self Cite

316

288

View full text Add to dashboard Cite

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“…4 Therefore, middle ribs of leaves were excised and leaf material was frozen in liquid nitrogen. After grinding, phytohormones 21,22 and scopoletin 23 were extracted from leaf tissue and analyzed as described previously. For the evaluation of resistance effects, scoring of symptom development at 6 and 12 d post infection (dpi) based on a six-category scale ("0, no symptoms" to "5, maximum necrosis") and determination of Pst growth in planta were performed.…”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

Cis- and trans-zeatin differentially modulate plant immunity

Großkinsky

Edelsbrunner²,

Pfeifhofer³

et al. 2013

Plant Signaling & Behavior

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“…Briefly, TSS were 175 extracted from 50 mg frozen leaves using 80% (v/v) ethanol at 80 C 176 for 1 h. After centrifugation, the supernatant was mixed with 177 1.5 mL of anthrone and incubated at 100 C during 10 min. Total phenolic compounds were determined according to the 187 protocol used by Singleton and Rossi (1965 Technologies) using the protocol described by Novák et al (2008). Water deficit reduced net CO 2 assimilation rate (A) ( Fig.…”

mentioning

confidence: 99%

Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit

Jesus

Meijón

Monteiro

et al. 2015

Environmental and Experimental Botany

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A B S T R A C TEucalyptus genus is the most widely planted hardwood tree, which productivity and development are limited by low water availability. Plant drought tolerance can be managed by adopting strategies, such as the exogenous application of salicylic acid. The main objective of the present study was to assess whether the exogenous foliar salicylic acid application would ameliorate the damages of water deficit on Eucalyptus globulus plants. Plants were watered at 70% (well water) or 15% (water deficit) of field capacity and four concentrations of salicylic acid (0, 0.75, 2.5 and 5.0 mM) were applied. Water potential, total chlorophylls and carotenoids contents, chlorophyll fluorescence parameters, leaf gas exchange, malondialdehyde, total soluble sugars, starch and total phenols contents were measured. The global hormonal content was quantified by ultra-performance liquid chromatography-mass spectrometry and specific local dynamics of indolacetic acid and abcisic acid were detected by immunolocalization in leaves. A multivariate statistical approach was used to get an overview of the plant physiological status. E. globulus water deficit response included growth rate decline associated with reduced in both water potential and leaf gas exchange parameters. Plant water deficit defence strategies led to an increase in total chlorophylls and carotenoids contents, lipid peroxidation, phenols and total soluble sugars. Six from the 18 hormones detected increased in water deficit plants. Exogenous salicylic acid application improved water deficit tolerance of E. globulus by improving water potential with a positive impact in primary metabolism (photosynthetic rate, soluble sugars) but also in secondary metabolism and defence mechanisms (higher total phenols and less lipid peroxidation) in the highest salicylic acid concentrations. Also, changes in endogenous levels of abscisic and salicylic acids, gibberellins 4 and 7, and specific cytokinins were found in water deficit plants with salicylic acid application. Our results indicated that salicylic acid application could be a potential chemical priming strategy to ameliorate water deficit effects on E. globulus plants.

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Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry

Cited by 216 publications

References 38 publications

A Subset of Cytokinin Two-component Signaling System Plays a Role in Cold Temperature Stress Response in Arabidopsis

A Subset of Cytokinin Two-component Signaling System Plays a Role in Cold Temperature Stress Response in Arabidopsis

Cis- and trans-zeatin differentially modulate plant immunity

Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit

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