Cytolethal Distending Toxin in Avian and Human Isolates of<i>Helicobacter pullorum</i>

Young, Vincent B.; Chien, Chih-Ching; Knox, Kimberly A.; Taylor, Nancy S.; Schauer, David B.; Fox, James G.

doi:10.1086/315705

Cited by 86 publications

(66 citation statements)

References 15 publications

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“…Taxa 2, 3 and 8 are presently unnamed and their taxonomic characterization awaits further study (Dewhirst et al, 2000). Young et al (2000b) have also proposed that the presence/absence of the cdtB gene may prove useful in the classification of microaerobic spiral bacteria. Their suggestion was based on experiments that included eight clinical isolates and three reference strains of H. pullorum.…”

Section: Discussionmentioning

confidence: 99%

Cytolethal distending toxin B gene (cdtB) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira

Kostia

Veijalainen

Hirvi

et al. 2003

Journal of Medical Microbiology

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The presence of the cytolethal distending toxin B gene (cdtB) was examined in eight Helicobacter sp. flexispira reference strains, Helicobacter trogontum ATCC 700114 T and 12 Finnish porcine H. trogontum strains and canine flexispira isolates. Part of the cdtB gene was amplified by PCR with degenerate primers VAT2 and DHF1, cloned and sequenced. The presence/absence of the cdtB gene as determined by PCR was confirmed by Southern hybridization and toxin production by HeLa cell-line experiments. PCR amplification resulted in approximately 700 bp fragments from Helicobacter sp. flexispira taxa 2 (ATCC 49314), 3 (ATCC 49320) and 8 (ATCC 43880, ATCC 49308, ATCC 43879), from six canine isolates as well as from the control strains Helicobacter bilis and Helicobacter hepaticus. The hybridization patterns of HaeIII-, HindIII-and AseI-digested chromosomal DNA confirmed the results of the PCR experiments. The cdtB-positive strains had effects ranging from weak to strong on HeLa cell cultures. PCR amplification from the reference strains Helicobacter sp. flexispira taxa 1 (ATCC 43968), 4 (ATCC 49310) and 5 (ATCC 43966) and H. trogontum (ATCC 700114 T ), and also six of the Finnish strains, was unsuccessful. No toxic effect on HeLa cells was evident when bacterial suspensions of PCR-negative strains were used for toxicity assay. Our results are in accordance with previous observations that the cdtB gene is not present in all Helicobacter species. Further, the presence/absence of the cdtB gene in Helicobacter sp. flexispira strains was in accordance with recent taxonomic analysis of the same strains, which suggests that it could serve as a useful marker in Helicobacter taxonomy.

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Section: Discussionmentioning

confidence: 99%

Cytolethal distending toxin B gene (cdtB) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira

Kostia

Veijalainen

Hirvi

et al. 2003

Journal of Medical Microbiology

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“…Until a few years ago, enteritis caused by these microorganisms were included within those caused by the Campylobacter genus due to their microscopic similarity (86). However, the development of new laboratory techniques as the fatty acid profile (87), mass spectrometry and the PCR (8) have made it possible to identify the germs more specifically.…”

Section: Acute Infectious Gastroenteritismentioning

confidence: 99%

“…Therefore, although the epidemiological chain has not been clearly defined (72), there appears to be a basis to consider food borne transmission of the gastroenteritis of H. pullorum as occurs with the Campylobacter species (4). Generally, they cause watery diarrhea with rectal bleeding in which it is believed that a cytotoxic toxin that has been isolated in certain cases of H. pullorum diarrhea could be involved, although not in other EHHs (86). In our center, where Helicobacter pullorum is being investigated systematically since April 2010, 9 cases that affected patients with a wide age spectrum have been identified (74).…”

Section: Acute Infectious Gastroenteritismentioning

confidence: 99%

Enterohepatic Helicobacter other than Helicobacter pylori

Mateos-Muñoz

Pérez-de-la-Serna

Ruiz-de-León

et al. 2013

Rev. esp. enferm. dig.

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The Helicobacter genus includes Gram negative bacteria which were originally considered to belong to the Campylobacter genus. They have been classified in a separate genus since 1989 because they have different biochemical characteristics, with more than 24 species having been identified and more still being studied.H. pylori is the best known. It has an important etiopathogenic role in peptic ulcer disease and gastric cancer. Enterohepatic Helicobacters (EHH) other than H. pylori colonize the bowel, biliary tree and liver of animals and human beings with pathogenic potential. The difficulties existing to correctly isolate these microorganisms limit the description of their true prevalence and of the diseases they cause. Many studies have tried to discover the different clinical implications of EHH. Diseases like chronic liver disease, autoimmune hepatitis, hepatocarcinoma, autoimmune hepatobiliary disease, biliary lithiasis, cholangiocarcinoma and gallbladder cancer, Meckel´s diverticulum, acute appendicitis and inflammatory bowel disease have been related with different EHH species with different results, although their prevalence is greater than in healthy subjects. However, these data are currently not sufficient to draw definitive conclusions. Finally, the best known role of EHH in bowel disease is production of acute and chronic diarrhea pictures initially referred to as Campylobacter. H. pullorum has been identified in patients with acute gastroenteritis. The correct identification of EHH as producers of infectious gastroenteritis is found in its antibiotic susceptibility. It is generally macrolidesusceptible and quinolone-resistant.

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“…In infected persons, no history of human contact with birds was reported so far. Nevertheless, recent studies demonstrated that H. pullorum could be an initiator of the intestinal inflammation, because of the releasing cytolethal distending toxin, leading to activation of kB-necrozing factor pathway in the mucous intestine cells [12] . However, Infection of apparently healthy finch and Australian parrots with H. pullorum indicated that these birds might be referred as a source enterohepatic pathogenic bacteria in humans and causing digestive problems to humans.…”

Section: Discussionmentioning

confidence: 99%

Evcil Kuşların Sekum İçeriklerinden Helicobacter pullorum 16S rRNA Geninin Amplifikasyonu

Gholami‐Ahangaran¹,

Azargoon²,

Anari³

2015

Kafkas Univ Vet Fak Derg

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For evaluation of pet birds as a resource of Helicobacter pullorum, 100 cecal content samples were collected from 50 finches and 50 Australian parrots, with no clinical signs suggestive of systemic and/or enteric disease, in Iran. DNA was extracted from cecal samples and PCR was carry out for identification of H. pullorum 16S rRNA gene. Results showed, H. pullorum DNA was detected in 3 of the 50 (6%) cecal samples of finch and 6 out of 50 (12%) cecal samples of Australian parrot. This study represents that finch and Australian parrot can be a source of H. pullorum infection and serve as reservoirs for the H. pullorum. Keywords: Australian Parrot, Finch, Helicobacter pullorum Evcil Kuşların Sekum İçeriklerinden Helicobacter pullorum 16S rRNA Geninin Amplifikasyonu ÖzetHelicobacter pullorum kaynağı olarak evcil kuşların değerlendirilmesi amacıyla İran'da sistemik veya enterit klinik belirtileri göstermeyen 50 ispinoz ve 50 Avustralya papağanından toplam 100 sekum içeriği örneği toplandı. Sekal örneklerden DNA ekstraksiyonu yapıldı ve H. pullorum 16S rRNA geninin identifikasyonu amacıyla PCR gerçekleştirildi. H. pullorum geni 50 ispinozun 3'ünde (%6) ve 50 Avustralya papağanının 6'sında (%12) belirlendi. Çalışmanın sonuçları ispinoz ve Avustralya papağanının H. pullorum enfeksiyonu kaynağı ve bu etken için reservuar olabileceğini göstermiştir.

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Cytolethal Distending Toxin in Avian and Human Isolates ofHelicobacter pullorum

Cited by 86 publications

References 15 publications

Cytolethal distending toxin B gene (cdtB) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira

Cytolethal distending toxin B gene (cdtB) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira

Enterohepatic Helicobacter other than Helicobacter pylori

Evcil Kuşların Sekum İçeriklerinden Helicobacter pullorum 16S rRNA Geninin Amplifikasyonu

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