Cytological and Biochemical Markers of Autophagy in Wheat Suspension Culture Cells under Abiotic Stress

Mazina, Anastasia; Dmitrieva, S. A.; Renkova, A. G.; Valitova, J. N.; Khabibrakhmanova, V.R.; Minibayeva, Farida

doi:10.26907/2542-064x.2020.4.541-556

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…Autophagy is a catabolic process strictly regulated at the molecular genetic level [ 207 ]. It is observed during starvation, stress, senescence, and some developmental stages.…”

Section: Carbon Starvationmentioning

confidence: 99%

Plant Heterotrophic Cultures: No Food, No Growth

Puzanskiy,

Romanyuk,

Kirpichnikova

et al. 2024

Plants

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Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are Nicotiana tabacum and Arabidopsis thaliana. Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the heterotrophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as glycerol, carbonic acids, starch, and mannitol have the ability to support growth occasionally, or in combination with another substrate. Culture growth is accompanied by processes of specialization, such as elongation growth. This determines the pattern of the carbon budget. Culture ageing is closely linked to substrate depletion, changes in medium composition, and cell physiological rearrangements. A lack of substrate leads to starvation, which results in a decrease in physiological activity and the mobilization of resources, and finally in the loss of viability. The cause of the instability of cultivated cells may be the non-optimal metabolism under cultural conditions or the insufficiency of internal regulation.

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“…Autophagy is a catabolic process strictly regulated at the molecular genetic level [ 207 ]. It is observed during starvation, stress, senescence, and some developmental stages.…”

Section: Carbon Starvationmentioning

confidence: 99%

Plant Heterotrophic Cultures: No Food, No Growth

Puzanskiy,

Romanyuk,

Kirpichnikova

et al. 2024

Plants

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“…Therefore, the aim of the present study was to identify potential protein targets of S-nitrosylation in the cells of wheat roots, in which autophagy was induced by the application of antimycin A, a mitochondrial inhibitor, and KNO2, a donor of NO. We have previously showed that antimycin A [25] and KNO2 [26] efficiently induce autophagy in wheat roots. To identify S-nitrosylated proteins, we applied a complex approach including polyacrylamide gel electrophoresis (PAGE), immunoblotting with monoclonal antibodies followed by protein identification in visualized electrophoretic zones (bands) using the bottom-up proteomics approach, i.e.…”

Section: Introductionmentioning

confidence: 99%

S-nitrosylated Proteins Involved in Autophagy in <em>Triticum aestivum</em> Roots: A Bottom-Up Proteomics Approach and In Silico Predictive Algorithms

Mazina,

Shumilina,

Gazizova

et al. 2023

Preprint

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Autophagy is a highly conserved catabolic process in eukaryotic cells. Reactive nitrogen species play roles as inductors and signaling molecules of autophagy. A key mechanism of NO-mediated signaling is S-nitrosylation, a posttranslational modification (PTM) of proteins at cysteine resi-dues. In the present work we analyzed the patterns of protein S-nitrosylation during the induc-tion of autophagy in Triticum aestivum roots. The accumulation of S-nitrosylated proteins in the cells during autophagy induced by KNO2 and antimycin A was visualized using monoclonal an-tibodies by Western blot analysis, and proteins were identified using a standard bottom-up pro-teomics approach. Protein S-nitrosylation is a labile and reversible PTM, and therefore SNO group can be lost during experimental procedures. Subsequent bioinformatic analysis using predictive algorithms and protein-ligand docking showed that identified proteins possess hypothetical S-nitrosylation sites. Analyzing protein-protein interaction networks enabled us to discover the targets that can directly interact with autophagic proteins, and those that can interact with them indirectly via key multifunctional regulatory proteins. In this study, we show that S-nitrosylation is a key mechanism of NO-mediated regulation of autophagy in wheat roots. A combination of in silico predictive algorithms with a mass spectrometry analysis provides a targeted approach for the identification of S-nitrosylated proteins.

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