Cytological and Cytochemical Investigations of the Adenohypophysis of the Sheep

Mikami, Shuji; Daimon, Tateo

doi:10.1679/aohc1950.29.427

Cited by 20 publications

(13 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The two acidophil types (orange and red) correspond well with those observed by other workers (Clarke and Purves 1960;van Blom 1965;Mikami and Daimon 1968). The aurantiphil (orange) type is similar to the cell identified as a somatotroph in the bovine by Dubois and Herlant (1968).…”

Section: Discussionsupporting

confidence: 89%

“…Apparent activity about the time of parturition and in early lactation supports the designation of erythrosinophils as the source of prolactin, suggested by Racadot (1963b). Mikami and Daimon (1968) described a similar cell in sheep.…”

Section: Discussionmentioning

confidence: 93%

“…They were AF-and AB-negative, and probably correspond to the delta 2 cells of Mikami and Daimon (1968). In wethers some of these cells were stained with acid fuchsin after Slidder's trichrome and in most gonadectomized animals a weak P AS-positive reaction was seen.…”

Section: Discussionmentioning

confidence: 95%

“…In wethers some of these cells were stained with acid fuchsin after Slidder's trichrome and in most gonadectomized animals a weak P AS-positive reaction was seen. Since pituitary FSH content is higher in wethers than ewes (Bindon and Tassell 1969), these cells may be involved in production of this hormone as postulated by Mikami and Daimon (1968). However, such designation must be considered tentative.…”

Section: Discussionmentioning

confidence: 99%

“…The treatment resulted in macroscopic and microscopic changes in the thyroid which suggested marked stimulation of the gland. It seems probable that the AF cell class in sheep includes the thyrotrophs (Mikami and Daimon 1968) but it may also include a gona.dotroph since these cells were prominent in castrated sheep.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Tinctorial Differentiation of the Cell Types in the Pars Anterior of the Sheep

Tassell¹,

Kennedy²

1972

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

Histological methods have been used to study the pars anterior of the sheep in a number of physiological states with the object of identifying the cellular sources of the hormones produced by this gland.It was possible to identify definitely four types of cell. Two of these were acidophils and stained red or orange after Herlant's tetrachrome. The orange cell showed little variation in different physiological states and probably produces growth hormone while the red was active about the time of parturition and appears to be the source of prolactin. Two classes of basophil, staining dark or light blue after tetrachrome, were identified. The former, which also were stained by complex basic dyes, were prominent after castration. The light blue cells were faintly PAS-positive when the PAS procedure was used and this reaction was slightly stronger after gonadectomy. Both types of basophil may be involved in gonadotrophin production but thyrotrophic and corticotrophic cells may also be included in these classes. Evidence supporting this theory is discussed.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Tinctorial Differentiation of the Cell Types in the Pars Anterior of the Sheep

Tassell¹,

Kennedy²

1972

Aust. Jnl. Of Bio. Sci.

View full text Add to dashboard Cite

show abstract

The pituitary gland of the musk shrew, Suncus murinus L. (insectivora), with special reference to the cytology of the adenohypophysis

Naik

Dominic

1972

Am. J. Anat.

View full text Add to dashboard Cite

The pituitary gland of the musk shrew, Suncus murinus L. was studied with special reference to the cytology of the adenohypophysis. In addition to the agranular cells (chromophobes ), seven morphologically and tinctorially distinct Nchromophilic cell types (four mucoid and three acidophilic) were observed in the pars anterior. These cells have been tentatively named as types one to seven. On the basis of their morphology, topography and staining reactions, cells of types one to six were compared, respectively, with the TSH, FSH, ICSH (L,H), LTH (prolactin), STH and ACTH cells of other mammals. The identity of type 7 cells could not be determined. Although mucoid cells (types 1, 2) art: more concentrated in the rostroventral region of the pars anterior, a definite "zona tuberalis" seems to be lacking. Since the musk shrew is not a seasonal breeder, the gonadotropic cells (types 2, 3 ) of the adenohypophysis do not seem to fluctuate in structure during the different times of the year. However, conspicuous hypertrophy, hyperplasia and intense secretory activity of type 4 cells during pregnancy and lactation reinforces the postulation that these cells are lactotropic in function. Follicular cysts and colloid in the residual cleft were observed in a few instances. Their possible origin and significance are discussed.Knowledge cif the morphology and cytology of the hypophysis in several groups of mammals has been enriched immensely i n recent years. (Purves, '61, '66; Herlant, '64, '65; Hansi:rom, '66; Girod, '66; Allanson and Parkes, '66). However, only scanty inform ation is available on the order Insectivora, except for the mole (Herlant, '64; Holmes, '66), desman (Herlant, '64) and hedgehog (e.g., Girod et al., '66, '67). The family Soricidae of this order does not seem to have received any attention, the (only study having been confined to brief description of the hypophysis of the short-tailed shrew, Blarina (Dawson, '63; Hanstrom, '66). The present paper presents a detailed anatomical description of the hypophysis and a comprehensive account on the cytology of the adenohypophysis of the musk shrew, Suncus ,murinus L. Banaras Hindu University c a m m s and adjacent areas were used. Included were 50 females in different stages of gestation, 12 pregnant and concurrently lactating females, 8 lactating females, 90 non-pregnant females and nearly 100 males. Most of the animals were sacrificed within 12 hours of capture; some were maintained in the laboratory for varying periods before sacrifice. Conspicuous seminiferous tubules visible through the tunica albuginea of the testes, and the presence of spermatozoa in the testes and the genital tract, were taken as indicators of sexual maturity in the male. Since some animals weighing 28-35 gm were pregnant, females weighing 40 gm or more were regarded as being sexually mature. The condition of the mammary glands, uteri and ovaries were considered in assessing the sexual maturity of the female. Pregnant females were classified into three groups (those of early, ...

show abstract

Seasonal changes in prolactin and growth hormone cells in the hypophyses of white‐tailed deer (Odocoileus virginianus borealis) studied by light microscopic immunocytochemistry and radioimmunoassay

et al. 1980

View full text Add to dashboard Cite

Numerous investigators have shown a relationship between basal serum prolactin (PRL) levels and photoperiod in domestic ungulates, and we have shown recently that a similar seasonal baseline serum PRL profile exists in both sexes of a captive but free-breeding population of white-tailed deer. In order to localize and describe the distribution and possible seasonal changes of PRL and growth hormone (GH) cells in the adenohypophyses of deer, we have used antisera raised against ovine PRL and bovine GH and the unlabeled antibody enzyme technique, in conjunction with point counting morphometry. In addition, we have measured seasonal changes in pituitary PRL concentrations in this same population, using a heterologous application of a recently developed homologous radioimmunoassay (RIA) for ovine PRL. Our results show that GH and PRL cells comprise two morphologically separate and immunologically distinct populations. Growth hormone cells were distributed evenly throughout the gland, except in the zona tuberalis, and males had a slightly greater volume density of immunoreactive GH than females; however, there were no differences between sexes in the average size of these cells, and little seasonal change was observed in these cell populations. In contrast to GH cells, the volume density of PRL cells, the size of PRL cells, and the pituitary concentration of RIA--measurable PRL all showed marked seasonal changes in deer of both sexes. These changes were well correlated with one another and with previously reported changes in basal serum levels (in these animals), in that they all reached zenith during midsummer, and nadir in midwinter. Our results demonstrate the necessity of reporting the time of year and latitude (to specify photoperiod) in studies concerning PRL cell physiology, particularly when dealing with any wild animal population.

show abstract

Cytological and Cytochemical Investigations of the Adenohypophysis of the Sheep

Cited by 20 publications

References 22 publications

Tinctorial Differentiation of the Cell Types in the Pars Anterior of the Sheep

Tinctorial Differentiation of the Cell Types in the Pars Anterior of the Sheep

The pituitary gland of the musk shrew, Suncus murinus L. (insectivora), with special reference to the cytology of the adenohypophysis

Seasonal changes in prolactin and growth hormone cells in the hypophyses of white‐tailed deer (Odocoileus virginianus borealis) studied by light microscopic immunocytochemistry and radioimmunoassay

Contact Info

Product

Resources

About