Cytological and enzymological characterization of adult human adipocyte precursors in culture.

Van, Robin L. R.; Bayliss, C. E.; Roncari, Daniel A. K.

doi:10.1172/jci108516

Cited by 241 publications

(106 citation statements)

References 28 publications

Supporting

Mentioning

102

Contrasting

Order By: Relevance

“…Later, this isolation procedure was modified for use in human models 17 . The collection of samples of adipose tissue, which was occasionally performed during other surgical procedures, has become more feasible since the advent of plastic surgery and liposuctioned.…”

Section: Introductionmentioning

confidence: 99%

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Fadel

Viana²,

Feitosa

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

Purpose:To evaluate different protocols to isolate stem cells from ovine umbilical cord blood and adipose tissue. Methods: There were used 5 samples of umbilical blood and 5 samples of perirenal adipose tissue from 10 female sheep. All the samples were obtained through surgery, to harvest aseptic samples. There were used 3 protocols for obtainment and culture of umbilical cord blood stem cells and 4 protocols for ovine adipose tissue stem cells. Results: It was possible to observe only one successful protocol for the obtainment of umbilical cord blood stem cells. When analyzing the techniques used to obtain adipose tissue stem cells, only one of the methods was effective as well. Through colony forming unit assay, there were obtained 58 colonies of cells after seven days in culture. Flow citometry tests revealed the cells were positive to CD44 and exhibited negative reaction to CD38, CD45, CD41/61. These cells showed a growth curve with very well defined phases LOG, LAG and PLATEAU. This phases are typically seem in mesenchymal stem cells growth curves. Conclusions: The isolation and culture of mesenchymal stem cells from ovine umbilical cord blood are complex and request more detailed assays. Stem cells from fat tissue sheep showed mesenchymal characteristics, according to their cell growth curve, ability to origin colonies of fibroblastoid cells and positive reactivity with the antibody CD44 by flow citometry. Key words: Stem Cells. Umbilical Cord. Adipose Tissue. Sheep. RESUMO Objetivo:Testar diferentes protocolos para o isolamento de células tronco a partir de sangue de cordão umbilical e tecido adiposo de ovinos. Métodos: Foram utilizadas cinco amostras de sangue de cordão umbilical e cinco amostras de tecido adiposo perirrenal de 10 fêmeas de ovelha. A coleta das amostras foi realizada através de procedimento cirúrgico para coleta do material de forma mais asséptica possível. Foram realizados três protocolos de isolamento e cultivo das células-tronco do cordão umbilical e quatro protocolos para o isolamento e cultivo das células-tronco de gordura de ovinos Resultados: Somente um dos protocolos utilizados para o isolamento das células-tronco de cordão umbilical foi efetivo. Dos quatro protocolos utilizados para isolamento das células-tronco de gordura, da mesma forma, apenas um obteve sucesso. Foi realizado o ensaio de unidades formadoras de colônias destas células, sendo contadas 58 colônias ao final de sete dias. Na citometria de fluxo essas células mostraram-se positivas para CD44 e negativas para CD38, CD45, CD41/61. Estas células apresentaram curva de crescimento com fases de LOG, LAG e PLATEAU bem definidas, características das curvas de crescimento das células-tronco de origem mesenquimal. Conclusões: O isolamento e cultivo das células-tronco mesenquimais do cordão umbilical de ovinos é de difícil realização, exigindo maiores ensaios e estudos profundos. Células tronco do tecido adiposo de ovelhas demonstraram características mesenquimais, de acordo com a curva de crescimento, habilidade de ...

show abstract

Section: Introductionmentioning

confidence: 99%

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Fadel

Viana²,

Feitosa

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…[3][4][5][6] As Green et al 7 reported that adipose tissue contains 'Preadipocytes' in 1974, a number of investigations have been reported regarding adipose tissue-derived stem/progenitor cells, so-called 'adipose-derived stromal cells (ADSCs). [3][4][5]8 Other adipogenic cell populations 3,9,10 have also been established with the multipotency of differentiation into not only mesenchymal lineage cells such as osteoblasts, 11,12 chondrocytes, 11,13,14 myocytes, 12 cardiomyocytes, 15 fibroblasts 3 and adipocytes 12 but also vascular lineage cells 15,16 such as endothelial, smooth muscle and circulating blood cells in stromal vascular fraction (SVF) isolated from adipose tissue. Among the variety of ADSCs, human multipotent adipose-derived stem (hMADS) cells 6 isolated from young donors show fast adherent cell characteristics in culture and have more extensive expansion capacity ex vivo over 200 passages.…”

mentioning

confidence: 99%

Local transplantation of human multipotent adipose-derived stem cells accelerates fracture healing via enhanced osteogenesis and angiogenesis

Shoji

Masaaki

Mifune

et al. 2010

Laboratory Investigation

View full text Add to dashboard Cite

Adipose tissue is one of the promising sources of multipotent stem cells in human. Human multipotent adipose-derived stem (hMADS) cells have recently been isolated and showed differentiation potential into multiple mesenchymal lineages in vitro and in vivo. On the basis of these evidences, we examined the therapeutic efficacy of hMADS cells for fracture healing in an immunodeficient rat femur non-union fracture model. Local transplantation of hMADS cells radiographically and histologically promoted fracture healing with significant improvement of biomechanical function at the fracture sites compared with local transplantation of human fibroblasts (hFB) or PBS administration. Histological capillary density and physiological blood flow by laser Doppler perfusion imaging were significantly greater in hMADS group than hFB and PBS groups. Expressions of intrinsic (rat) bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and angiopoietin-1 in peri-fracture tissue were upregulated in hMADS group than other groups. In addition, presence of BMP-2 or VEGF activated the proliferation and migration of hMADS cells in vitro. These results indicate that hMADS cells stimulate the interaction between the transplanted cells and the resident cells stronger than other cells, and they promote fracture healing more effectively. Furthermore, immunohistochemistry for human-specific antibodies revealed direct differentiation of hMADS cells into osteoblasts or endothelial cells in newly formed callus or vasculature, respectively. RT-PCR for human-specific primers for osteogenic/endothelial markers also disclosed osteogenic and vasculogenic plasticity of the transplanted hMADS cells at the early stage of fracture healing. The present results suggest that transplantation of hMADS cells may become a useful strategy for cell-based bone regeneration in the future clinical setting.

show abstract

“…28,29 When 80 ± 90% conuent, the cells were trypsinised and subcultured in SCM into T75 cm 2¯a sks (two¯asks per treatment) for RNA extraction. SCM comprised DMEMaHam's F12 with 10% foetal bovine serum, 100 Uaml penicillin, 0.1 mgaml streptomycin and 2 mM glutamine.…”

Section: Preadipocyte Isolation and Culturementioning

confidence: 99%

Adipose depot-specific expression of cIAP2 in human preadipocytes and modulation of expression by serum factors and TNFα

et al. 2001

View full text Add to dashboard Cite

BACKGROUND: Visceral obesity is more closely associated with many deleterious metabolic sequelae than obesity per se. The identi®cation of properties that distinguish fat cells of the omentum from adipocytes situated elsewhere in the body may lead to the development of therapeutic strategies targeting visceral obesity. We have previously demonstrated that cIAP2 mRNA is signi®cantly overexpressed in omental (Om) compared with subcutaneous (Sc) adipocytes. This molecule is involved in the TNFa signalling pathway and may inhibit apoptosis. OBJECTIVE: To examine the effect of serum agents and TNFa upon cIAP2 mRNA expression in human primary culture preadipocytes. DESIGN: Paired omental and subcutaneous adipose tissue biopsies were obtained from 11 patients, nine female and two male, with ages ranging from 29 to 82. These were cultured in either serum containing medium or serum-free medium with or without the addition of TNFa for 4 h and mRNA levels analysed by quantitative reverse-transcription polymerase chain reaction. RESULTS: When human preadipocytes were cultured in a de®ned medium containing foetal calf serum the Om cells had a greater level of expression of cIAP2 mRNA than Sc cells from the same individual (mean 3.5-fold higher Sc b Om; P`0.01). However, when serum was removed from this media for a transitory period the level of cIAP2 mRNA decreased in the omental depot such that Sc preadipocytes had greater cIAP2 expression than their Om counterparts. Addition of TNFa induced a large increase in mRNA levels of cIAP2 (mean 20-fold).CONCLUSIONS: These results demonstrate that cIAP2 is expressed in a depot-speci®c manner in human preadipocytes and that levels of expression are regulated by serum factors and TNFa. Thus there may be intrinsic differences between preadipocyte cells from different adipose depots and this may play a role in the regulation of body fat distribution via the modulation of fat cell apoptosis.

show abstract

Cytological and enzymological characterization of adult human adipocyte precursors in culture.

Cited by 241 publications

References 28 publications

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Local transplantation of human multipotent adipose-derived stem cells accelerates fracture healing via enhanced osteogenesis and angiogenesis

Adipose depot-specific expression of cIAP2 in human preadipocytes and modulation of expression by serum factors and TNFα

Contact Info

Product

Resources

About