Cytological characterization of sex chromosomes and ribosomal DNA location in &lt;i&gt;Anastrepha&lt;/i&gt; species (Diptera, Tephritidae)

Goday, Clara; Selivon, Denise; Perondini, André Luiz Paranhos; Greciano, Patricia G.; Ruiz, María Fernanda

doi:10.1159/000091931

Cited by 40 publications

(79 citation statements)

References 47 publications

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“…Solferini and Morgante (1987) described the mitotic chromosomes of this species using C-banding techniques, and recently Goday et al (2006), using sequential fluorochrome staining, showed that blocks of heterochromatin have differential affinities for the fluorochromes DAPI and CMA 3 ( Figure 1C). For the comparisons that will be made in the present analysis, the description of the A. serpentina chromosomes will be briefly summarized below and shown in the idiogram in Figure 3.…”

Section: Anastrepha Serpentinamentioning

confidence: 95%

“…At least 25 larvae of the first generation of each species were used for chromosome preparations. Since the chromosomes of A. serpentina were described before by Goday et al (2006), only a few chromosome preparations were made for the present analysis in order to compare them with the karyotypes of the other two species.…”

Section: Chromosome Preparation Staining and In Situ Hybridizationmentioning

confidence: 99%

“…Mitotic chromosome preparations were obtained according to the methods used for Anastrepha species by Goday et al (2006). Briefly, the imaginal discs and neural ganglia were dissected, transferred to 0.2% sodium citrate for 13 min, fixed in methanol, acetic acid and water (11:11:2. v/v) and squashed in 45% acetic acid.…”

Section: Chromosome Preparation Staining and In Situ Hybridizationmentioning

confidence: 99%

“…Later, the size and distribution of heterochromatic blocks were also included in the descriptions Morgante, 1987,1990;Morgante et al, 1993Morgante et al, , 1996Basso and Manso, 1998;Basso et al, 2003;Selivon et al, 2004Selivon et al, , 2005aSelivon et al, , 2005b. More recently, the cytochemical properties of the heterochromatin as well as the location of ribosomal sequences were investigated in eight Anastrepha species (Goday et al, 2006), providing new tools for the cytotaxonomy of this group of flies.…”

Section: Introductionmentioning

confidence: 99%

“…The chromosomes of A. serpentina have been previously analyzed by these techniques (Goday et al, 2006). Using the markers generated by these procedures, the chromosomes of the three species were compared in order to obtain new data that might contribute to a better understanding of the evolution of their karyotypes and of the taxonomic relationships among them.…”

Section: Introductionmentioning

confidence: 99%

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Karyotype relationships among Anastrepha bistrigata, A. striata and A. serpentina (Diptera, Tephritidae)

et al. 2007

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The species of Anastrepha are arranged into 17 intrageneric groups. Recently, it was proposed that two species of the striata group, Anastrepha striata and A. bistrigata, might be realocated to serpentina group. Anastrepha bistrigata and A. serpentina have an X 1 X 2 Y/X 1 X 1 X 2 X 2 sex chromosome system while A. striata has a XY/XX system. It was previously proposed that the karyotype of A. bistrigata could be derived from that of A. striata by an Y:A fusion, and that the karyotype of A. serpentina would be derived from another, hypothetical karyotype. In the present report sequential staining with DAPI and chromomycin A3 (CMA 3 ), followed by C-banding, revealed that the C-banded heterochromatic blocks of the sex chromosomes of A. bistrigata have different affinities to fluorochromes in comparison to the chromosomes of A. striata, from which they have hypothetically derived. The chromosomes of A. serpentina show substantial differences in their cytochemical properties compared to their A. bistrigata and A. striata counterparts. The FISH technique showed that the ribosomal gene sequences are located in DAPI-or DAPI/CMA 3 -positive heterochromatic blocks of the sex chromosomes, one site on the Y chromosome and one site on the X chromosome (X 1 in A. bistrigata and A. serpentina). The data suggest that the karyotype of A. striata and A. bistrigata could be derived from a common ancestral karyotype, while the A. serpentina karyotype probably has a distinct origin.

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Section: Anastrepha Serpentinamentioning

confidence: 95%

Section: Chromosome Preparation Staining and In Situ Hybridizationmentioning

confidence: 99%

Section: Chromosome Preparation Staining and In Situ Hybridizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Karyotype relationships among Anastrepha bistrigata, A. striata and A. serpentina (Diptera, Tephritidae)

et al. 2007

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Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)

Drosopoulou

Nakou

Šíchová³

et al. 2012

Genetica

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The olive fruit fly, Bactrocera oleae, has a diploid set of 2n = 12 chromosomes including a pair of sex chromosomes, XX in females and XY in males, but polytene nuclei show only five polytene chromosomes, obviously formed by five autosome pairs. Here we examined the fate of the sex chromosomes in the polytene complements of this species using fluorescence in situ hybridization (FISH) with the X and Y chromosome-derived probes, prepared by laser microdissection of the respective chromosomes from mitotic metaphases. Specificity of the probes was verified by FISH in preparations of mitotic chromosomes. In polytene nuclei, both probes hybridized strongly to a granular heterochromatic network, indicating thus underreplication of the sex chromosomes. The X chromosome probe (in both female and male nuclei) highlighted most of the granular mass, whereas the Y chromosome probe (in male nuclei) identified a small compact body of this heterochromatic network. Additional hybridization signals of the X probe were observed in the centromeric region of polytene chromosome II and in the telomeres of six polytene arms. We also examined distribution of the major ribosomal DNA (rDNA) using FISH with an 18S rDNA probe in both mitotic and polytene chromosome complements of B. oleae. In mitotic metaphases, the probe hybridized exclusively to the sex chromosomes. The probe signals localized a discrete rDNA site at the end of the short arm of the X chromosome, whereas they appeared dispersed over the entire dot-like Y chromosome. In polytene nuclei, the rDNA was found associated with the heterochromatic network representing the sex chromosomes. Only in nuclei with preserved nucleolar structure, the probe signals were scattered in the restricted area of the nucleolus. Thus, our study clearly shows that the granular heterochromatic network of polytene nuclei in B. oleae is formed by the underreplicated sex chromosomes and associated rDNA.

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Antibiotic treatment reduces fecundity and nutrient content in females of Anastrepha fraterculus (Diptera: Tephritidae) in a diet dependent way

Goane

Salgueiro

Pereyra

et al. 2022

Journal of Insect Physiology

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Cytological characterization of sex chromosomes and ribosomal DNA location in <i>Anastrepha</i> species (Diptera, Tephritidae)

Cited by 40 publications

References 47 publications

Karyotype relationships among Anastrepha bistrigata, A. striata and A. serpentina (Diptera, Tephritidae)

Karyotype relationships among Anastrepha bistrigata, A. striata and A. serpentina (Diptera, Tephritidae)

Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)

Antibiotic treatment reduces fecundity and nutrient content in females of Anastrepha fraterculus (Diptera: Tephritidae) in a diet dependent way

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