Cytological details of Luffa cylindrica (L.)M.Roem. a meiotically disturbed diploid

Jamwal, Mamata; Sharma, Namrata

doi:10.1007/s13237-016-0186-z

Cited by 5 publications

(2 citation statements)

References 13 publications

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“…Suitably sized DNA fragments were selected for library (ID: CL1000100146) construction and processed for paired‐end (PE) sequencing. L. cylindrica was reported to be a diploid (2n = 26) plant (Jamwal & Sharma, ). The quality of the Hi‐C library was evaluated by HiC‐Pro by identifying valid interaction pairs and invalid interaction pairs (Servant et al, ).…”

Section: Methodsmentioning

confidence: 99%

Long‐read sequencing and de novo assembly of the Luffa cylindrica (L.) Roem. genome

Zhang

Ren

Zhang

et al. 2020

Molecular Ecology Resources

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Sponge gourd (Luffa cylindrica (L.) Roem.) or luffa is a diploid herbaceous plant with 26 chromosomes (2n = 26) and belongs to the family Cucurbitaceae. To address the limited knowledge of the genome of Luffa species, the chromosome‐level genome of L. cylindrica was assembled and analysed using PacBio long reads and Hi‐C data. We combined Hi‐C data with a draft genome assembly to generate chromosome‐length scaffolds. Thirteen scaffolds corresponding to the 13 chromosomes were assembled from 1,156 contigs to a final size of 669 Mb with a contig N50 size of 5 Mb and a scaffold N50 size of 53 Mb. After removing redundant sequences, 416.31 Mb (62.18% of the genome) of repeat sequences was detected. Subsequently, 31,661 protein‐coding genes with an average of 5.69 exons per gene were identified in the L. cylindrica genome using de novo methods, transcriptome data and homologue‐based approaches. In addition, 27,552 protein‐coding genes (87.02%) were annotated in five databases. According to the phylogenetic analysis, L. cylindrica is closely related to Cucurbita and Cucumis species and diverged from their common ancestor ~28.6–67.1 million years ago. Genome collinearity analysis was performed in Cucurbita moschata, Cucumis sativus and L. cylindrica, and it demonstrated a high degree of conserved gene order in these three species. The completeness of the genome will provide high‐quality genomic knowledge on breeding and reveal genetic variation in L. cylindrica.

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Section: Methodsmentioning

confidence: 99%

Long‐read sequencing and de novo assembly of the Luffa cylindrica (L.) Roem. genome

Zhang

Ren

Zhang

et al. 2020

Molecular Ecology Resources

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“…In this study, both monoecious and dioecious species were utilized. L. acutangula , L. cylindrica , and L. graveolens are monoecious vines, bearing separate male and female flowers on the same plants ( Jamwal and Sharma, 2017 ), while L. echinata is dioecious and bears male and female flowers on the different plants. The time gap between the first staminate flower’s appearance at the lower node and the first female flower on the middle node varies among species (13–15 days in L. cylindrica , 16–17 days in L. acutangula , and 12–15 days in L. graveolens ).…”

Section: Introductionmentioning

confidence: 99%

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

Nagar,

Gowthami,

Sureja

et al. 2023

Front. Plant Sci.

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This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica, and L. echinata, and BK medium with 3% sucrose ideal for L. graveolens. Overestimation in staining tests compared to in vitro pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40 min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and −20°C) and duration. Cryopreserved pollen at −196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across Luffa species, facilitating long-term storage and its use in crop improvement efforts.

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First Report on Aneusomaty and Chromosomal Chimeras in Male Track of Luffa cylindrica (L.) M. Roem

Bano

Sharma

2020

Natl. Acad. Sci. Lett.

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Cytological details of Luffa cylindrica (L.)M.Roem. a meiotically disturbed diploid

Cited by 5 publications

References 13 publications

Long‐read sequencing and de novo assembly of the Luffa cylindrica (L.) Roem. genome

Long‐read sequencing and de novo assembly of the Luffa cylindrica (L.) Roem. genome

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency

First Report on Aneusomaty and Chromosomal Chimeras in Male Track of Luffa cylindrica (L.) M. Roem

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