Cytological diagnosis by fine needle sampling without aspiration

Abstract

The merits of a simplified cytological method of fine needle sampling without aspiration are compared to those of the classical fine needle aspiration techniques in a series of benign and malignant mammary tumors which were subsequently proved histologically. A comparable cellular yield was obtained by both techniques. In a series of 635 benign and malignant breast tumors examined in 1981 with fine needle alone, insufficient cellular yield was recorded in 5.5% of the lesion. The same incidence (6%) was recorded with aspiration techniques in 7877 benign and malignant mammary tumors examined from 1954 to 1980. With the new technique, trauma is reduced and a better perception of the tumor and of its consistency is directly obtained.

Comparison of fixation procedures for fluorescent quantitation of DNA content using image cytometry

“…Fine needle samples of ten breast carcinomas were harvested in 1 ml HBSS/BSA according to the procedure of Zajdela et al (22). The cells were immediately fixed and treated as above.…”

Plasminogen activator inhibitor-1 (PAI-1) is not related to response to neoadjuvant chemotherapy in breast cancer

“…Responders were defined by either a complete response (CR, disappearance of clinically palpable disease) or partial response (PR, reduction of more than 50% of the product of the two largest tumour diameters). Stable disease was defined by no change in tumour size and progressive disease 537 (26) 29 (42) 3 (4) 55 (80) 12 (17) 2 (3) 7 (10) 30 (43) 28 (41) 4 (6) 35 (51) 32 (46) 2 (3) 40 (58) (Zajdela et al, 1987) and processed as previously described for S-phase analysis by flow cytometry (Remvikos et al, 1991). Assays PAI-I concentration was measured by ELISA (American Diagnostica) on hypertonic (0.4 M potassium cholride) cytosolic extracts of the drill biopsy sample before chemotherapy and of the surgical sample after chemotherapy, as described previously (Romain et al, 1995 DNA flow cytometry DNA flow cytometry analysis was performed on the pretreatment fine-needle sample of the primary tumour according to a technique previously described (Remvikos et al, 1991).…”

Biopsje cienkoigłowe guzków tarczycy pod kontrolą USG — porównanie wydajności próbki za pomocą różnych technik pobierania próbek, rozmiarów igieł, z/bez analizy cytologicznej na miejscu

“…In this method, cells are detached by the cutting edge of the needle and are conducted into the lumen by capillary forces [18]. The absence of the vacuum effect of aspiration results in a significant reduction in trauma to the nodule and surrounding thyroid tissues and thus reduces blood contamination and yields higher quality material [18,21]. The capillary technique is therefore recommended, especially for nodules found to be highly vascular on colour Doppler imaging [4,10,11].…”