Cytological dissection of barley genome by the gametocidal system

Endo, Takashi R.

doi:10.1270/jsbbs.59.481

Cited by 11 publications

(6 citation statements)

References 36 publications

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“…This approach was subsequently applied to barley (Shi and Endo 1999) and rye (Friebe et al 2000). The chromosome 2C c induces frequent breakage in the centromeric region of barley chromosomes, with high rate of telocentric chromosomes and whole-arm translocations (Endo 2009 (Said et al 2012) of H. chilense. Alien introgression is an important strategy in both common (Khlestkina 2014) and durum wheats (Ceoloni et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

et al. 2014

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Chromosome 7H ch of Hordeum chilense carries the Phytoene synthase 1 (Psy1) gene encoding the first step in the carotenoid biosynthetic pathway. As such it can be used in the improvement of seed carotenoid content in wheat. Four introgressions of chromosome 7H ch into wheat have been characterized by in situ hybridization of labeled DNA probes and by several sets of DNA markers. Chromosome-specific SSR were used for the identification of wheat chromosomes. Besides 113 conserved orthologous set (COS) markers were tested for homoeologous group 7, of which 97 amplified in H. chilense and 32 were polymorphic between H. chilense and wheat, and 28 expressed sequence tag (EST) barley markers previously allocated to chromosome 7. A total of 60 markers (32 COS and 28 EST) were allocated to chromosome 7H ch with 28 assigned to 7H ch S and 22 to 7H ch L. A combination of in situ probing and marker genotyping have shown that among the four introgressions there was a substitution line 7H ch (7D), a ditelosomic addition line for the long arm of 7H ch and two homozygous centric translocations 7H ch SÁ2DS and 7H ch SÁ5AL. The Psy1 gene was localized on the short arm of 7H ch . The positions of markers from the international barley consortium map (IBSC2012) were determined and the comparative arm location between H. chilense and H. vulgare is discussed. The genetic stocks characterized here include new wheat-H. chilense recombinations useful for genetic studies and with a potential for breeding.

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Section: Discussionmentioning

confidence: 99%

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

et al. 2014

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“…This system, the gametocidal (Gc) system, involves alien chromosomes called Gc chromosomes, which were introduced into common wheat from certain wild species belonging to the genus Aegilops. This system proved to be effective in inducing structural rearrangements in the barley chromosomes added to common wheat, as well as in common wheat chromosomes (Endo 2009). The rearranged chromosomes thus induced include deletions of barley chromosomes and translocations between the barley and wheat chromosomes.…”

Section: Chromosome Pairing In Wheat 3 Barley Hybridsmentioning

confidence: 98%

“…The rearranged chromosomes thus induced include deletions of barley chromosomes and translocations between the barley and wheat chromosomes. Lines carrying rearranged barley chromosomes are designated as 'dissection lines' (Endo 2009). Schubert et al (1998) developed wheat-barley translocations from wheat/ barley disomic addition lines by exploiting the gametocidal effect of the 2C chromosome of Aegilops cylindrica.…”

Section: Chromosome Pairing In Wheat 3 Barley Hybridsmentioning

confidence: 99%

Wheat–barley hybridization: the last 40 years

2013

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Several useful alien gene transfers have been reported from related species into wheat (Triticum aestivum), but very few publications have dealt with the development of wheat/barley (Hordeum vulgare) introgression lines. An overview is given here of wheat 9 barley hybridization over the last forty years, including the development of wheat 9 barley hybrids, and of addition and translocation lines with various barley cultivars. A short summary is also given of the wheat 9 barley hybrids produced with other Hordeum species. The meiotic pairing behaviour of wheat 9 barley hybrids is presented, with special regard to the detection of wheatbarley homoeologous pairing using the molecular cytogenetic technique GISH. The effect of in vitro multiplication on the genome composition of intergeneric hybrids is discussed, and the production and characterization of the latest wheat/barley translocation lines are presented. An overview of the agronomical traits (b-glucan content, earliness, salt tolerance, sprouting resistance, etc.) of the newly developed introgression lines is given. The exploitation and possible use of wheat/barley introgression lines for the most up-to-date molecular genetic studies (transcriptome analysis, sequencing of flow-sorted chromosomes) are also discussed.

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“…cylindrica. Gametocidal genes have been used to produce structural chromosome aberrations in wheat [36,37], barley [38,39] and rye [40]. In H. chilense, structural changes have been previously obtained for…”

Section: Discussionmentioning

confidence: 99%

Development of wheat—Hordeum chilense Chromosome 2Hch Introgression Lines Potentially Useful for Improving Grain Quality Traits

Palomino

Cabrera

2019

Agronomy

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The chromosome 2Hch of Hordeum chilense. has the potential to improve seed carotenoid content in wheat as it carries a set of endosperm carotenoid-related genes. We have obtained structural changes in chromosome 2Hch in a common wheat (Triticum aestivum L. “Chinese Spring”) background by crossing a wheat double disomic substitution 2Hch(2D) and 7Hch(7D) line with a disomic addition line carrying chromosome 2Cc from Aegilops cylindrica Host.. Seven introgressions of chromosome 2Hch into wheat were characterized by fluorescence in situ hybridization (FISH) and DNA markers. Chromosome-specific simple sequence repeats (SSRs) were used for identifying wheat chromosomes. In addition, we tested 82 conserved orthologous set (COS) markers for homoeologous group 2, of which 65 amplified targets in H. chilense and 26 showed polymorphism between H. chilense and wheat. A total of 24 markers were assigned to chromosome 2Hch with eight allocated to 2HchS and 16 to 2HchL. Among the seven introgressions there was a disomic substitution line 2Hch(2D), a ditelosomic addition line for the 2HchL arm, an isochromosome for the 2HchL arm, a homozygous centromeric 2HchS·2DL translocation, a double monosomic 2HchS·2DL plus 7HchS·D translocation, a homozygous centromeric 7HchS·2HchL translocation and, finally, a 2HchL·7HchL translocation. Wheat—H. chilense macrosyntenic comparisons using COS markers revealed that H. chilense chromosome 2Hch exhibits synteny to wheat homoeologous group 2 chromosomes, and the COS markers assigned to this chromosome will facilitate alien gene introgression into wheat. The genetic stocks developed here include new wheat—H. chilense recombinations which are useful for studying the effect of chromosome 2Hch on grain quality traits.

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Cytological dissection of barley genome by the gametocidal system

Cited by 11 publications

References 36 publications

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

Cytological and molecular characterization of wheat-Hordeum chilense chromosome 7Hch introgression lines

Wheat–barley hybridization: the last 40 years

Development of wheat—Hordeum chilense Chromosome 2Hch Introgression Lines Potentially Useful for Improving Grain Quality Traits

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