Cytological Identification and Chromosome Constitution Analyses of Ten Oc-toploid 
            <i>Trititrigia</i> Accessions

Qi, Xiaobin; Bao, Yinguang; Li, Xingfeng; Qian, Zhao-Guo; Wang, Ruixia; Wu, Ke; Wang, Honggang

doi:10.3724/sp.j.1006.2017.00967

Cited by 2 publications

(5 citation statements)

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“…intermedium to these three Trititrigia octoploids and that TE261-1 also exhibited moderate resistance to aphids. In addition, the chromosomal constitution of TE261-1, TE266-1 and TE346-1 is different from that of other TE series of Trititrigia octoploids reported previously [21,26,28]. Therefore, these accessions could be employed as bridge parents for crossing with wheat to develop addition, substitution or translocation lines to generate new rust-, powdery mildew-and aphid-resistant germplasms for wheat breeding.…”

Section: Discussionmentioning

confidence: 83%

“…2), which might have formed via the centromere breakage of one pair of J S -genome chromosomes. J-St translocated chromosomes also exist in many TE series of Trititrigia octoploids [21,26,28], but J S acrocentric chromosomes were first discovered in the TE series of Trititrigia octoploids. The formation of these two types of variations might occur through chromosome breakage and fusion when F 1 hybrids obtained through distant hybridization are backcrossed with hexaploid wheat [34].…”

Section: Discussionmentioning

confidence: 99%

“…intermedium to wheat. These Trititrigia octoploids include TAF46 [23], 78,829 [24], the 'Zhong' series of Trititrigia octoploids [25] and the TE series of Trititrigia octoploids [20,21,[26][27][28]. The TE series of Trititrigia octoploids was developed from hybrid progenies between Th.…”

Section: Introductionmentioning

confidence: 99%

“…intermedium, such as large spikes, multi-florets, multitillers, and good resistance to stripe rust, powdery mildew and leaf rust. Thus, this TE series of Trititrigia octoploids represent an excellent genetic resource for wheat improvement [21,[26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

“…intermedium, it was widely used in the identification of Th. intermedium-derived wheat germplasms [19,21,28,33].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of chromosome constitution in three wheat - Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomes

Cui

Xing

et al. 2021

BMC Plant Biol

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Background Thinopyrum intermedium (2n = 6x = 42) is an important wild perennial Triticeae species exhibiting many potentially favorable traits for wheat improvement. Wheat-Th. intermedium partial amphiploids serve as a bridge to transfer desirable genes from Th. intermedium into common wheat. Results Three octoploid Trititrigia accessions (TE261–1, TE266–1, and TE346–1) with good resistances to stripe rust, powdery mildew and aphids were selected from hybrid progenies between Th. intermedium and the common wheat variety ‘Yannong 15’ (YN15). Genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and multicolor GISH (McGISH) analyses demonstrated that the three octoploid Trititrigia possess 42 wheat chromosomes and 14 Th. intermedium chromosomes. The 14 alien (Th. intermedium) chromosomes belong to a mixed genome consisting of J-, JS- and St-genome chromosomes rather than a single J, JS or St genome. Different types of chromosomal structural variation were also detected in the 1A, 6A, 6B, 2D and 7D chromosomes via FISH, McGISH and molecular marker analysis. The identity of the alien chromosomes and the variationes in the wheat chromosomes in the three Trititrigia octoploids were also different. Conclusions The wheat-Th. intermedium partial amphiploids possess 14 alien chromosomes which belong to a mixed genome consisting of J-, JS- and St- chromosomes, and 42 wheat chromosomes with different structural variations. These accessions could be used as genetic resources in wheat breeding for the transfer of disease and pest resistance genes from Th. intermedium to common wheat.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…intermedium, it was widely used in the identification of Th. intermedium-derived wheat germplasms [19,21,28,33].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of chromosome constitution in three wheat - Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomes

Cui

Xing

et al. 2021

BMC Plant Biol

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Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes

Cui

Zhang

Qin

et al. 2018

Genome

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Synthesized oligonucleotides (oligos) can be used as effective probes similar to plasmid clones for chromosome identification in fluorescence in situ hybridization (FISH) analysis, making oligo FISH a simpler and more efficient molecular cytogenetic technique for studying plants. In this study, multiplex oligonucleotide probes, including pSc119.2-1, pAs1-4, (GAA), (AAC), and pTa71, were combined and used in FISH to identify chromosomes in common wheat, Thinopyrum intermedium, and a wheat - Th. intermedium amphiploid TE256-1. In comparison with general FISH probes, signals generated by the multiplex probes were more abundant, colorful, and characteristic. Combining the results of genomic in situ hybridization (GISH) with FISH, Th. intermedium chromosomes and alien chromosomes in TE256-1 could be classified and identified more precisely, especially the J- and J-genome chromosomes. Moreover, based on the FISH results using multiplex probes, more structural variations in wheat chromosomes of TE256-1 were detected. The results indicated that multiplex oligo probes would have a wide range of application prospects in the creation and identification of wheat - Th. intermedium germplasms.

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Cytological Identification and Chromosome Constitution Analyses of Ten Oc-toploid Trititrigia Accessions

Cited by 2 publications

References 0 publications

Characterization of chromosome constitution in three wheat - Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomes

Characterization of chromosome constitution in three wheat - Thinopyrum intermedium amphiploids revealed frequent rearrangement of alien and wheat chromosomes

Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes

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