Cytological observations of “nonproducer” Rous sarcoma cells

Stefano, Henry S. Di; Dougherty, Robert M.

doi:10.1016/0042-6822(65)90116-9

Cited by 14 publications

(10 citation statements)

References 10 publications

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“…Ultrastructural studies of other RSVinduced mammalian tumors have revealed aggregates of cytoplasmic crystalline material in tumors oi the rhesus monkey (3) and hamster (4) and budding virus particles in intracerebral tumors of the dog (5) but no cytoplasmic virus particles or unusual changes in cytoplasmic membranes. Chicken fibroblasts infected by defective RSV of the Bryan strain contained parallel arrays of endoplasmic reticulum associated with particles thought to be glycogen (6,7), but similar arrays of hypertrophied endoplasmic reticulum have been reported in tumor cells of diverse origin (8,9).…”

Section: Discussionsupporting

confidence: 53%

Unique Cytoplasmic Membranes in Rous Sarcoma Virus-Induced Tumors of a Subhuman Primate

Smith

Deinhardt

1968

The Journal of Cell Biology

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Although the natural host of the Rous sarcoma virus (RSV) is the chicken, many strains of the virus have induced tumors in various mammals. Recently, a New World monkey, the marmoset (Saquinus sp.), was added to the list of animals susceptible to RSV oncogenesis (1). This report deals with a unique membranous cytoplasmic structure found in cells from two intracerebral and four subcutaneous tumors which developed following neonatal inoculation with the Schmidt-Ruppin strain of RSV.

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Section: Discussionsupporting

confidence: 53%

Unique Cytoplasmic Membranes in Rous Sarcoma Virus-Induced Tumors of a Subhuman Primate

Smith

Deinhardt

1968

The Journal of Cell Biology

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“…The number of virus particles in the cells was estimated at 1/10 to 1/100 the number found in culture of Rous sarcoma cells releasing infectious virus. The morphology of the NP cells in the electron microscope was similar to that of cells releasing infectious particles of RSV (55). The failure of NP cells to produce infectious virus indicated that transformation was dependent only on infection by RSV.…”

Section: Virus Particles In Np Cellsmentioning

confidence: 66%

The early history of tumor virology: Rous, RIF, and RAV

Rubin

2011

Proc. Natl. Acad. Sci. U.S.A.

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One hundred years ago Peyton Rous recovered a virus, now known as the Rous sarcoma virus (RSV), from a chicken sarcoma, which reproduced all aspects of the tumor on injection into closely related chickens. There followed recovery of causal viruses of tumors of different morphology from 4 more of 60 chicken tumors. Subsequent studies in chickens of the biology of the first RSV isolated moved slowly for 45 y until an assay of ectodermal pocks of the chorioallantoic membrane of chicken embryos was introduced. The inadequacies of that assay were resolved with the production of transformed foci in cultures of chicken fibroblasts. There followed a productive period on the dynamics of RSV infection. An avian leukosis virus (ALV) was found in some chicken embryos and named resistance-inducing factor (RIF) because it interferes with RSV. Its epidemiology in chickens is described. Another ALV was found in stocks of RSV and called Rous-associated virus (RAV). Cells preinfected with RAV interfere with RSV infection, but RSV does not produce infectious virus unless RAV is added during or after RSV infection. Intracellular RAV provides the infectious coat for the otherwise defective RSV. The coat determines the antigenicity, host range, and maturation rate of RSV. RSV particles carry reverse transcriptase, an enzyme that converts their RNA into DNA and allows integration into the cell's DNA, where it functions as a cellular gene. This was the bridge that joined the biological era to the molecular era. Its relation to oncogenes and human cancer is discussed.

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“…The presence of such viral material in nonproducing Rous sarcoma cells suggested that morphologically identifiable precursors of RSV might be found in such cells, and prompted the electron microscopic investigations reported in this communication. Similar expectations led Dougherty and Di Stefano (10,12) to study the ultrastructure of virus-producing and nonproducing Rous sarcoma cells. The principal observations coming from the two laboratories are in excellent agreement.…”

mentioning

confidence: 63%

Electron Microscopy of Chick Fibroblasts Infected by Defective Rous Sarcoma Virus and Its Helper

Courington

Vogt

1967

J Virol

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Nonproducing Rous sarcoma cells of the chicken and their virus-producing as well as uninfected counterparts were studied with an electron microscope. The structural peculiarities of transformed cells included cytoplasmic annulate lamellae, aggregates of membrane-bound, glycogen-like granules, and empty, virus-like shells. Of 69 individual lines of nonproducing Rous sarcoma cells, 64 contained small numbers of viral particles. These particles were morphologically indistinguishable from mature avian tumor virus but lacked demonstrable infectivity. In sessile normal and leukosis virus-infected fibroblasts, microtubules and fibrils occurred in parallel arrays at the periphery of the cytoplasm. This cortical organization was absent from rounded Rous sarcoma cells. The characteristics of microtubular arrangement seemed to reflect differences in the locomotory activity of normal and transformed cells.

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Cytological observations of “nonproducer” Rous sarcoma cells

Cited by 14 publications

References 10 publications

Unique Cytoplasmic Membranes in Rous Sarcoma Virus-Induced Tumors of a Subhuman Primate

Unique Cytoplasmic Membranes in Rous Sarcoma Virus-Induced Tumors of a Subhuman Primate

The early history of tumor virology: Rous, RIF, and RAV

Electron Microscopy of Chick Fibroblasts Infected by Defective Rous Sarcoma Virus and Its Helper

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