Cytological Studies in &lt;i&gt;Vicia faba&lt;/i&gt; L. Treated with Asafoetida

Das, TusharKanti; Raj, A. Sudharsan; Rao, B. Dayakar

doi:10.1508/cytologia.33.100

Cited by 14 publications

(10 citation statements)

References 5 publications

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“…Some of the other chemicals which produced stickiness were 8 ethoxy caffeine (Kihlman 1955), potassium cyanide (Lily 1958), podophyllin (Hussein and Hakeem 1960) and asafoetida (Narasimba Das et al 1968).…”

mentioning

confidence: 99%

Cytological effects of Achillea Fragrantissima extract on Allium cepa and Vicia faba.

1978

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Many plant extracts such as resorcinol, gloriosine , ergot alkaloid, eserine, podophyllin, asafoetida, lathyrogens...etc.were reported by several authors as causing cytological abnormalities.The aim of this work was to study the cytological effect of water extract from Achillea fragrantissima which have a medicinal value , on Allium cepa and Vicia faba. Material and methodsPreparation of the extract A weighed quantity (3, 5 and 9g) of the plant was boiled in 100ml tap water for 5 minutes. The evaporated water was replaced by tap water to the original volume (100ml), then the extract was filtered.Secondry roots of Vicia faba (var. Rebaya 40) 1-3cm in length were treated with the different concentrations of the extract (3, 5 and 9%) for 1 and 2 hours for each concentration.Allium cepa bulbs were grown in tap water at room temperature for 4-6 days . When the roots were 2-4cm in length they were treated as in Vicia.The roots were cut, fixed in Carnoy's fixative (1-3 acetic-alcohol) for 24h then stored in 70% alcohol.Another group of Vicia and A. cepa roots were taken off the extract solution after every time treatment (I and 2 h) for each concentration, washed with running water and transfered back to tap water for 24, 48 and 72 hours. Simultaneously control plants were grown in tap water for each of the different periods. The re covered roots were cut, fixed, stained and examined.Mitotic index was calculated as the average number of dividing cells from 10 different root tips, for each treatment 10000-20000 cells were counted. Results and discussionAs shown in Table 1, the Achillea extract affected the mitotic phases. Ac cumulation of prophases was observed in nearly all the direct treatments of A. cepa and Vicia except in 1h 9% where increase of ana-telophases was noted. The accumulation of prophases was on the expense of the other phases. This indi cates a delay in the spindle formation.1 Present address, Faculty of Education, Doha, Qatar.

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confidence: 99%

Cytological effects of Achillea Fragrantissima extract on Allium cepa and Vicia faba.

1978

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“…The formation of ball metaphase may be due to the localized activity of spindle apparatus at the centre, so that the chromosomes were arranged in such a way that their centromeres remain at the equator and arms radiating in different directions and orienting in the form of a ball (Morgan, 2006). The improper functioning of spindle apparatus causes the diagonal orientation of chromosomes (Das et al, 1968). Star shaped arrangement may be due to the clumping of daughter chromosomes into star like structures near the polar region of the cell (Amer, 1965).…”

Section: Results:-mentioning

confidence: 99%

Mosquito Larvicidal and Cytotoxic Studies on Annona Reticulata L.

SoumyaS¹,

Ramachandran²,

DineshRaj³

et al. 2017

IJAR

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“…Since then, many alkaloids were tested by several investigators, among them, caffeine (Kihlman 1955), podo phyllin (Hussein and Hakeem 1960), asafoetida (Narasimha Das et al 1968), mor phine sulphate (Kabarity et al 1974) and vinblastine (Ciulei 1967, Srivastava et al 1973and Segawa and Kondo 1978. in one cell of Gasteria ernestii-ruschii root.…”

Section: Abnormalitiesmentioning

confidence: 99%

Cytological effects of Vinca alkaloids I. Effect of vinblastine and yohimbine on meiosis of Vicia faba.

Shehab

1985

CYTOLOGIA

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Cytological Studies in <i>Vicia faba</i> L. Treated with Asafoetida

Cited by 14 publications

References 5 publications

Cytological effects of Achillea Fragrantissima extract on Allium cepa and Vicia faba.

Cytological effects of Achillea Fragrantissima extract on Allium cepa and Vicia faba.

Mosquito Larvicidal and Cytotoxic Studies on Annona Reticulata L.

Cytological effects of Vinca alkaloids I. Effect of vinblastine and yohimbine on meiosis of Vicia faba.

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