Cytological Studies of 4 Species of the Genus Kobresia (Cyperaceae) Collected from Nepal Himalaya

Yano, Okihito; Ikeda, Hiroshi; Watson, Mark; Rajbhandari, Keshab R.

doi:10.1508/cytologia.76.137

Cited by 3 publications

(5 citation statements)

References 9 publications

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“…Multiple origins from the same diploid progenitor species have been reported for bryophytes (Wyatt et al ., ), ferns (Ranker et al ., ) and angiosperms (Brochmann, Soltis & Soltis, ; Parisod & Besnard, ; Wu et al ., ), which have continuously increased intra‐autopolyploid diversity by introducing new maternal lineages (Soltis & Soltis, ). Therefore, even if we rejected an allopolyploid origin for K. pygmaea following Heilborn (), multiple origins from the same diploid ancestor or introgression from other species potentially played a role in the diversification history of K. pygmaea , and possibly in the entire genus (Waterway, ; Zhang, ; Yano et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…The resulting diploid chromosome number 2 n = 52 is not a multiple of x = 16 or x = 18, and thus x = 26 may represent a third basic number. Chromosome numbers of K. curvata (2 n = 50, Yano et al ., ) and K. duthiei (2 n = c . 84, Yano et al ., ) could represent multiples (Table ).…”

Section: Discussionmentioning

confidence: 99%

“…Chromosome numbers of K. curvata (2 n = 50, Yano et al ., ) and K. duthiei (2 n = c . 84, Yano et al ., ) could represent multiples (Table ). In any case, K. myosuroides is the species with the most variable chromosome counts ranging from 2 n = 4 x = 52 to 66.…”

Section: Discussionmentioning

confidence: 99%

“…One exception based on chromosome counts is a possible allopolyploid origin for K. nepalensis from ancestors such as K. myosuroides (Vill.) Fiori and K. esenbeckii (Kunth) Noltie (Yano et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Ploidy in the alpine sedgeKobresia pygmaea(Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry

et al. 2014

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Polyploidy is a fundamental mechanism in evolution, but is hard to detect in taxa with agmatoploidy or aneuploidy. We tested whether a combination of chromosome counting, microsatellite analyses and flow cytometric measurements represents a suitable approach for the detection of basic chromosome numbers and ploidy in Kobresia (Cyperaceae). Chromosome counting resulted in 2n = 64 for Kobresia pygmaea and K. cercostachys, 2n = 58 and 64 for K. myosuroides, and 2n = 72 for K. simpliciuscula. We characterized eight microsatellite loci for K. pygmaea, which gave a maximum of four alleles per individual. Cross-species amplification was tested in 26 congeneric species and, on average, six of eight loci amplified successfully. Using flow cytometry, we confirmed tetraploidy in K. pygmaea. Basic chromosome numbers and ploidy were inferred from chromosome counts and the maximum number of alleles per locus. We consider the basic numbers as x = 16 and 18, with irregularities derived from agmatoploidy and aneuploidy. Across all Kobresia taxa, ploidy ranged from diploid up to heptaploid. The combination of chromosome counts and microsatellite analyses is an ideal method for the determination of basic chromosome numbers and for inferring ploidy, and flow cytometry is a suitable tool for the identification of deviating cytotypes.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Ploidy in the alpine sedgeKobresia pygmaea(Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry

et al. 2014

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“…Combined application of chromosome counts, new microsatellite markers and flow cytometry confirmed tetraploidy in C. parvula (2n = 4x = 64) 5 . Chromosome numbers of Kobresia esenbeckii (2n = 66), Kobresia duthiei (2n = 84), Kobresia curvata (2n = 50), Kobresia schoenoides (2n = 32) were reported and it is concluded that chromosomal evolution in the unispicate Kobresia species may have been caused by both polyploidy and aneuploidy 6 .…”

Section: Introductionmentioning

confidence: 99%

Draft genomes assembly and annotation of Carex parvula and Carex kokanica reveals stress-specific genes

Bao

Liao

et al. 2022

Sci Rep

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Kobresia plants are important forage resources on the Qinghai-Tibet Plateau and are essential in maintaining the ecological balance of grasslands. Therefore, it is beneficial to obtain Kobresia genome resources and study the adaptive characteristics of Kobresia plants on the Qinghai-Tibetan Plateau. Previously, we have assembled the genome of Carex littledalei (Kobresia littledalei), which is a diploid with 29 chromosomes. In this study, we assembled genomes of Carex parvula (Kobresia pygmaea) and Carex kokanica (Kobresia royleana) via using Illumina and PacBio sequencing data, which were about 783.49 Mb and 673.40 Mb in size, respectively. And 45,002 or 36,709 protein-coding genes were further annotated in the genome of C. parvula or C. kokanica. Phylogenetic analysis indicated that Kobresia in Cyperaceae separated from Poaceae about 101.5 million years ago after separated from Ananas comosus in Bromeliaceae about 117.2 million years ago. C. littledalei and C. parvula separated about 5.0 million years ago, after separated from C. kokanica about 6.2 million years ago. In this study, transcriptome data of C. parvula at three different altitudes were also measured and analyzed. Kobresia plants genomes assembly and transcriptome analysis will assist research into mechanisms of plant adaptation to environments with high altitude and cold weather.

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Angiosperm Diversity in Nepal

Bhandari

2024

Plant and Vegetation

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Cytological Studies of 4 Species of the Genus Kobresia (Cyperaceae) Collected from Nepal Himalaya

Cited by 3 publications

References 9 publications

Ploidy in the alpine sedgeKobresia pygmaea(Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry

Ploidy in the alpine sedgeKobresia pygmaea(Cyperaceae) and related species: combined application of chromosome counts, new microsatellite markers and flow cytometry

Draft genomes assembly and annotation of Carex parvula and Carex kokanica reveals stress-specific genes

Angiosperm Diversity in Nepal

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