Cytological studies of recombination in rhesus males

Hassold, Terry; Hansen, Terah A.; Hunt, Patricia A.; VandeVoort, Catherine A.

doi:10.1159/000207519

Cited by 26 publications

(31 citation statements)

References 37 publications

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“…Both species have average MLH1 foci counts that approximate their autosomal chromosome numbers (Table 1), indicating that very rigid regulatory mechanisms must be in place to ensure that one (and usually only one) crossover is allocated to a given chromosome. In addition, both of these species have fewer MLH1 foci than chromosome arms (Table 1), complementing previous assertions that only one crossover per chromosome is necessary for correct homolog segregation Hassold et al 2009), at least in some taxa.…”

Section: Resultssupporting

confidence: 84%

“…A minimum of one crossover per chromosome arm is required to ensure the correct alignment of homologous chromosomes at the metaphase plate and their proper segregation during meiosis I, although recent studies have suggested that this constraint could be relaxed to one crossover per chromosome Hassold et al 2009). The average number of recombination events per autosome or per autosomal arm still varies markedly among the eight rodent taxa examined here (Table 1), suggesting that observed variation in raw MLH1 foci counts does not solely derive from differences in gross genome organization among rodent species.…”

Section: Resultsmentioning

confidence: 99%

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Evolution of the Genomic Recombination Rate in Murid Rodents

Dumont

Payseur

2011

Genetics

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Although very closely related species can differ in their fine-scale patterns of recombination hotspots, variation in the average genomic recombination rate among recently diverged taxa has rarely been surveyed. We measured recombination rates in eight species that collectively represent several temporal scales of divergence within a single rodent family, Muridae. We used a cytological approach that enables in situ visualization of crossovers at meiosis to quantify recombination rates in multiple males from each rodent group. We uncovered large differences in genomic recombination rate between rodent species, which were independent of karyotypic variation. The divergence in genomic recombination rate that we document is not proportional to DNA sequence divergence, suggesting that recombination has evolved at variable rates along the murid phylogeny. Additionally, we document significant variation in genomic recombination rate both within and between subspecies of house mice. Recombination rates estimated in F 1 hybrids reveal evidence for sex-linked loci contributing to the evolution of recombination in house mice. Our results provide one of the first detailed portraits of genomic-scale recombination rate variation within a single mammalian family and demonstrate that the low recombination rates in laboratory mice and rats reflect a more general reduction in recombination rate across murid rodents.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Evolution of the Genomic Recombination Rate in Murid Rodents

Dumont

Payseur

2011

Genetics

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“…As expected, longer chromosomes displayed more MLH1 foci and a significant correlation between the autosomal SC length and number of MLH1 foci was found. A similar covariance was observed in humans [Lynn et al, 2002] and rhesus monkeys [Hassold et al, 2009]. The distribution of recombination events along individual bivalents was influenced by interference, as reported previously for other mammalian species [Froenicke et al, 2002;Sun et al, 2004;Basheva et al, 2008;Borodin et al, 2008;Yang et al, 2011].…”

Section: Discussionsupporting

confidence: 84%

“…This difference might be caused by interindividual variability or by different scoring criteria, because signals at the ends of SCs and at SC overlaps were not counted by Hart et al The frequency of spermatocytes with one or more autosomal bivalents lacking recombination (8.3-15.7%) was within the range reported for other mammals (5% in humans, Sun et al [2006b]; 16.8% in rhesus monkeys, Hassold et al [2009]). …”

Section: Discussionmentioning

confidence: 95%

A Comparative Study of Meiotic Recombination in Cattle (Bos taurus) and Three Wildebeest Species (Connochaetes gnou, C. taurinus taurinus and C. t. albojubatus)

Vozdová

Sebestova

Kubı́čková

et al. 2013

Cytogenet Genome Res

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The karyotypic evolution in the family Bovidae is based on centric fusions of ancestral acrocentric chromosomes. Here, the frequency and distribution of meiotic recombination was analyzed in pachytene spermatocytes from Bos taurus (2n = 60) and 3 wildebeest species (Connochaetes gnou, C. taurinus taurinus and C. t. albojubatus) (2n = 58) using immunofluorescence and fluorescence in situ hybridization. Significant differences in mean numbers of recombination events per cell were observed between B. taurus and members of the genus Connochaetes (47.2 vs. 43.7, p < 0.001). The number of MLH1 foci was significantly correlated with the length of the autosomal synaptonemal complexes. The average interfocus distance was influenced by interference. The male recombination maps of bovine chromosomes 2 and 25 and of their fused homologues in wildebeests were constructed. A significant reduction of recombination in the fused chromosome BTA25 was observed in wildebeests (p = 0.005). This was probably caused by interference acting across the centromere, which was significantly stronger than the intra-arm interference. This comparative meiotic study showed significant differences among the species from the family Bovidae with the same fundamental number of autosomal arms (FN_a = 29) which differ by a single centric fusion.

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“…In meiosis, heterodimers of MLH1 with another MutL DNA repair homologue, MLH3, are essential for wild-type levels of crossing over in budding yeast, mammals and plants [Baker et al, 1996;Hunter and Borts, 1997;Lipkin et al, 2002;Jackson et al, 2006] The discovery that MLH1 and MLH3 form foci on chromosomes during meiotic prophase, and that these foci numbers and distribution correlate with the known frequency and distribution of COs in mice and humans [Baker et al, 1996;Lipkin et al, 2002] allowed cytological mapping of CO events along individual chromosomes [Froenicke et al, 2002]. Analysis of MLH1 foci along the meiotic chromosome axes provided information on genetic recombination rates and the nature of genetic interference in mammals [Anderson et al, 1999;Lynn et al, 2002;Hassold et al, 2004;de Boer et al, 2006;Cheng et al, 2009;Hassold et al, 2009].…”

mentioning

confidence: 99%

An Easy Protocol for Studying Chromatin and Recombination Protein Dynamics during Arabidopsisthaliana Meiosis: Immunodetection of Cohesins, Histones and MLH1

Chelysheva¹,

Grandont²,

Vrielynck³

et al. 2010

Cytogenet Genome Res

141

136

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Plant meiosis studies have enjoyed a fantastic boom in recent years with the use of Arabidopsis thaliana as a model not only for molecular genetics and genomics but also for cytogenetics. In this article we describe a new protocol for immunolabelling meiotic proteins that allows the detection of a large range of proteins on strongly spread chromosomes throughout the entire meiotic process. We used this method to immunodetect MLH1, a crucial component of the meiotic recombination machinery, and found that it can be visualised as foci from pachytene to diakinesis, where it co-localises with chiasmata. The mean MLH1 foci number per meiotic cell at diakinesis was 8.4 for WS-4 and 9.95 for Col-0, with the number of foci per bivalent ranging from 1 to 5. We also analysed MLH1 distribution within bivalents and found that they were not restricted to specific chromosomal regions. The analysis of MLH1 foci formation in the Atzip4 mutant, where class I crossover (CO) formation is prevented, revealed that residual chiasmata were not labelled by MLH1, strongly suggesting that MLH1 antibodies only label class I COs in Arabidopsis. It thus appears that the ‘obligatory CO’ is systematically labeled by MLH1 and is generated through the class I pathway.

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Cytological studies of recombination in rhesus males

Cited by 26 publications

References 37 publications

Evolution of the Genomic Recombination Rate in Murid Rodents

Evolution of the Genomic Recombination Rate in Murid Rodents

A Comparative Study of Meiotic Recombination in Cattle <b><i>(Bos taurus)</i></b> and Three Wildebeest Species <b><i>(Connochaetes gnou, C. taurinus taurinus</i></b> and <b><i>C. t. albojubatus)</i></b>

An Easy Protocol for Studying Chromatin and Recombination Protein Dynamics during <i>Arabidopsis</i><i>thaliana</i> Meiosis: Immunodetection of Cohesins, Histones and MLH1

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