Cytologie et cytophysiologie des cellules interstitielles de l'epinoche : Gasterosteus aculeatus L. Etude au microscope electronique

Follenius, E

doi:10.1016/0016-6480(68)90120-2

Cited by 25 publications

(4 citation statements)

References 30 publications

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“…As this enzyme is necessary to convert 11-ketoandrostenedione into 11-ketotestosterone, its presence in an extratesticular site may explain why 11-ketotestosterone is the main androgen in plasma while testes produce mainly 11-ketoandrostenedione in the reproductive period (Borg et al 1989). Confirmation of the presence of extratesticular androgen synthesis in the male stickleback also comes from the fact that 11-ketoandrostenedione in plasma increases, together with 11-hydroxyandrostenedione, during the postreproductive period, and reaches a peak in winter , while its production by the testes in the postreproductive period and in winter is very low, in agreement with the disappearance of Leydig cells (Gottfried & Van Mullem, 1967 ;Follenius, 1968). Furthermore, Mayer et al (1990 b) found that castration did not reduce 11-ketoandrostenedione plasma level.…”

Section: mentioning

confidence: 68%

“…In fact in the testes of this species, spermatogenesis is inhibited by androgens (Borg, 1981 ;Andersson et al 1988) and takes place in the postreproductive period after the disappearance of the Leydig cells (whose androgenic function is well known) from the testicular stroma. These cells appear again in the springtime prereproductive period (Gottfried & Van Mullem, 1967 ;Follenius, 1968). Nevertheless during the winter quiescence the plasma contains androgens which are evidently produced in some extratesticular site.…”

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confidence: 96%

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Ultrastructural and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus, Teleostea), in relation to the reproductive annual cycle

2001

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The steroidogenic interrenal cells in the adrenal homologue of the male stickleback (Gasterosteus aculeatus) were studied in relation to the reproductive cycle by means of histological and ultrastructural observations, and using histochemical methods for the localisation of 3β-hydroxysteroid dehydrogenase (3βHSD) and 17β-hydroxysteroid dehydrogenase (17βHSD). To determine the various stages of the reproductive cycle, the testes were also examined by histological and histochemical methods (3βHSD). The results indicate that in this teleost the interrenal cells can undergo a cycle in which phases characterised by different cytological aspects are observed. During this cycle there is a renewal of organelles, in particular mitochondria and SER. Periodic degenerative processes are also found. Organelle cytology showed that the cell cycle has at least 3 different aspects during the year. An analogy with some cytological aspects of the adrenal zonation in mammals is possible. It is postulated that the interrenal gland activity could substitute or supplement androgen production by the testes.

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Section: mentioning

confidence: 68%

mentioning

confidence: 96%

Ultrastructural and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus, Teleostea), in relation to the reproductive annual cycle

2001

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“…Thus, Leydig cells in the testis of this species showed morphological characteristics of a typical steroidogenic cell. Exceptionally, however, a few authors (Follenius & Porte, 1960 ; Follenius, 1968 ; Gresik et al, 1973 ) reported that lipid droplets were absent in healthy Leydig cells of teleost species. Therefore, the presence or absence of lipid droplets in the cytoplasm of Leydig cells varied with teleost species.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Studies of Germ Cell Development and the Functions of Leydig Cells and Sertoli Cells associated with Spermatogenesis in Kareius bicoloratus (Teleostei, Pleuronectiformes, Pleuronectidae)

2016

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The ultrastructures of germ cells and the functions of Leydig cells and Sertoli cells during spermatogenesis inmale Kareius bicoloratus (Pleuronectidae) were investigated by electron microscope observation. Each of the well-developed Leydig cells during active maturation division and before spermiation contained an ovoid vesicular nucleus, a number of smooth endoplasmic reticula, well-developed tubular or vesicular mitochondrial cristae, and several lipid droplets in the cytoplasm. It is assumed that Leydig cells are typical steroidogenic cells showing cytological characteristics associated with male steroidogenesis. No cyclic structural changes in the Leydig cells were observed through the year. However, although no clear evidence of steroidogenesis or of any transfer of nutrients from the Sertoli cells to spermatogenic cells was observed, cyclic structural changes in the Sertoli cells were observed over the year. During the period of undischarged germ cell degeneration after spermiation, the Sertoli cells evidenced a lysosomal system associated with phagocytic function in the seminiferous lobules. In this study, the Sertoli cells function in phagocytosis and the resorption of products originating from degenerating spermatids and spermatozoa after spermiation. The spermatozoon lacks an acrosome, as have been shown in all teleost fish spermatozoa. The flagellum or sperm tail of this species evidences the typical 9+2 array of microtubules.

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“…Using electrical stimulation and drugs, Nilsson (1970) concluded that in Gadus morhua cholinergic fibres innervate the testis, while the existence of adrenergic fibres has not been proved. Presence of nerve fibres and terminals has been observed in the testicular interstitium in three teleost species (Follenius, 1964;Gresik, 1973;van den Hurk, 1974). After ultrastructure, the observed fibres were considered to be either adrenergic or cholinergic.…”

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confidence: 96%

Spermatogonia of rainbow trout: III. in vitro study of the proliferative response to extracellular ATP and adenosine

Loir

1999

Mol. Reprod. Dev.

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Unlike in higher vertebrates, in fish it is not known whether the nerve supply of the testis can influence testicular functions or not. In addition to neurotransmitters, nerve terminals may release ATP and adenosine in the extracellular medium. On the assumption that these molecules might be released by fibers innervating the teleost testis, it is possible that they participate in the control of testicular function and, maybe, in the control of spermatogonia (Go) proliferation. This study addresses this issue. We have investigated the ability for extracellular ATP and adenosine to influence the in vitro incorporation, either basal or GTH‐, IGF‐I‐ and suramin‐stimulated, of 3H‐thymidine (3H‐Tdr) by trout Go. Mixed suspensions of somatic and germ cells prepared from testes, which were immature or spermatogenetic, were cultured usually for 4.5 days in the presence or not of the tested molecules; 3H‐Tdr was added during the last day in culture. In our cell culture conditions, 25 to 250 μM adenosine, ATP, ADP, and AMP stimulated the 3H‐Tdr incorporation by Go from prespermatogenetic testes and from testes starting spermatogenesis, in a dose‐dependent way. The effect of these molecules decreased when the testes were more advanced in spermatogenesis and it became inhibiting when the testes were in mid‐spermatogenesis. Five′‐N‐ethylcarboxamidoadenosine (NECA) was as potent as adenosine in stimulating or inhibiting 3H‐Tdr incorporation, while R‐N6‐(2‐phenylisopropyl)adenosine (R‐PIA) always had a marked inhibiting effect. Adenosine 5′‐O‐(3‐thiotriphosphate) (ATPγS; 25–200 μM), a non‐hydrolysable analogue of ATP, which had no effect on Go from prespermatogenetic testes (collected October–February) and from testes in advanced spermatogenesis, stimulated 3H‐Tdr incorporation by Go from testes at the beginning of spermatogenesis very efficiently. The order of potency of the different ATP analogues was as follows: ATPγS > ATP ≅ α,β‐methylene‐ATP > UTP > 2‐methylthio‐ATP. These data suggest that A2 adenosine receptors and P2 receptors would be present on unidentified testicular cells. The stimulating effect of adenosine/ATP was additive with that of either GTH‐I or IGF‐I or suramin when the cells were from testes at the beginning of spermatogenesis, but adenosine suppressed their effect when the cells were from testes in mid‐spermatogenesis. In conclusion, our results suggest that in the trout extracellular adenosine and ATP are able to influence the in vitro proliferation of Go, and are potential candidates for mediating the possible influence of the nervous system on the induction, speeding up, then slowing down of spermatogenesis. Mol. Reprod. Dev. 53:443–450, 1999. © 1999 Wiley‐Liss, Inc.

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Cytologie et cytophysiologie des cellules interstitielles de l'epinoche : Gasterosteus aculeatus L. Etude au microscope electronique

Cited by 25 publications

References 30 publications

Ultrastructural and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus, Teleostea), in relation to the reproductive annual cycle

Ultrastructural and histochemical study on the interrenal cells of the male stickleback (Gasterosteus aculeatus, Teleostea), in relation to the reproductive annual cycle

Ultrastructural Studies of Germ Cell Development and the Functions of Leydig Cells and Sertoli Cells associated with Spermatogenesis in Kareius bicoloratus (Teleostei, Pleuronectiformes, Pleuronectidae)

Spermatogonia of rainbow trout: III. in vitro study of the proliferative response to extracellular ATP and adenosine

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