Cytologie fine des cellules interstitielles du testicule du poissonLebistes reticulatus R.

Follenius, E; Porte, A

doi:10.1007/bf02178980

Cited by 32 publications

(4 citation statements)

References 2 publications

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“…Thus, Leydig cells in the testis of this species showed morphological characteristics of a typical steroidogenic cell. Exceptionally, however, a few authors (Follenius & Porte, 1960 ; Follenius, 1968 ; Gresik et al, 1973 ) reported that lipid droplets were absent in healthy Leydig cells of teleost species. Therefore, the presence or absence of lipid droplets in the cytoplasm of Leydig cells varied with teleost species.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Studies of Germ Cell Development and the Functions of Leydig Cells and Sertoli Cells associated with Spermatogenesis in Kareius bicoloratus (Teleostei, Pleuronectiformes, Pleuronectidae)

2016

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The ultrastructures of germ cells and the functions of Leydig cells and Sertoli cells during spermatogenesis inmale Kareius bicoloratus (Pleuronectidae) were investigated by electron microscope observation. Each of the well-developed Leydig cells during active maturation division and before spermiation contained an ovoid vesicular nucleus, a number of smooth endoplasmic reticula, well-developed tubular or vesicular mitochondrial cristae, and several lipid droplets in the cytoplasm. It is assumed that Leydig cells are typical steroidogenic cells showing cytological characteristics associated with male steroidogenesis. No cyclic structural changes in the Leydig cells were observed through the year. However, although no clear evidence of steroidogenesis or of any transfer of nutrients from the Sertoli cells to spermatogenic cells was observed, cyclic structural changes in the Sertoli cells were observed over the year. During the period of undischarged germ cell degeneration after spermiation, the Sertoli cells evidenced a lysosomal system associated with phagocytic function in the seminiferous lobules. In this study, the Sertoli cells function in phagocytosis and the resorption of products originating from degenerating spermatids and spermatozoa after spermiation. The spermatozoon lacks an acrosome, as have been shown in all teleost fish spermatozoa. The flagellum or sperm tail of this species evidences the typical 9+2 array of microtubules.

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Section: Discussionmentioning

confidence: 99%

Ultrastructural Studies of Germ Cell Development and the Functions of Leydig Cells and Sertoli Cells associated with Spermatogenesis in Kareius bicoloratus (Teleostei, Pleuronectiformes, Pleuronectidae)

2016

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“…The latter seems to bc a possibility in the case of cholesterol synthesis bv the liver, for a considerable increase in granular reticulum has been found in hepatic cells stimulated to very high levels of cholesterol production (22,21). In the interstitial ceils of the testis, however, electron micrographs leave no doubt that the reticulum is agranular in opossum, man (16), albino rat (9), guinea pig (15), and the fish Lebistes (17). The major contribution of testicular interstitial cells to the microsome fraction would therefore consist of small vesicles resulting from the fragmentation of the agranular endoplasmic re ticulum.…”

Section: Discussionmentioning

confidence: 99%

The Normal Fine Structure of Opossum Testicular Interstitial Cells

Christensen

Fawcett

1961

The Journal of Cell Biology

249

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The interstitial tissue of the opossum testis includes interstitial or Leydig cells, macrophages, and small cells which morphologically resemble mesenchymal cells. The latter are thought to give rise to mature interstitial cells. The most prominent feature of the interstitial cell cytoplasm is an exceedingly abundant agranular endoplasmic reticulum. This reticulum is generally in the form of a meshwork of interconnected tubules about 300 to 450 A in diameter, but occasionally it assumes the form of flattened, fenestrated cisternae resembling those of pancreatic acinar cells, except for the lack of ribonucleoprotein particles on the surface of the membranes. The interstitial cells vary considerably in their cytoplasmic density. The majority are quite light, but some appear extremely dense, and in addition usually have a more irregular cell surface, with numerous small pseudopodia. These differences may well reflect variations in physiological state. Cytoplasmic structures previously interpreted as "crystalloids" consist of long bundles of minute parallel tubules, each about 180 A in diameter, which seem to be local differentiations of the endoplasmic reticulum. The mitochondria are rod-shaped, and contain a moderately complex internal membrane structure, and also occasional large inclusions that are spherical and homogeneous. The prominent juxtanuclear Golgi complex contains closely packed flattened sacs and small vesicles. The results of the present study, coupled with biochemical evidence from other laboratories, make it seem highly probable that the agranular endoplasmic reticulum is involved in the synthesis of the steroid hormones produced by the interstitial cell. This finding therefore constitutes one of the first functions of the agranular reticulum for which there is good morphological and biochemical evidence.

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“…Somewhat similar results were obtained by McArthur (1952) in rats treated with luteinizing hormone, while Sluiter, van Oordt & Mighorst (1950) found that the Leydig cells of hypophysectomized frogs underwent a kind of fatty degeneration, characterized by the development of excessive lipid vacuolization. The view that the terminal stages of the secretory cycle, characterized by the appearance of the larger lipid cholesterol-positive droplets, is really a degenerative phase, has been supported by Follenius & Porte (1960). The young and active stage of the cell was described in their electron microscope studies as having a round nucleus, small nucleolus, numerous mitochondria and cytoplasmic vesicles, while in the light microscope, the cytoplasm showed eosinophilic and sudanophilic inclusions.…”

Section: Discussionmentioning

confidence: 99%

“…The nucleolus is characteristically large and osmiophilic and the nucleus also shows a distinct reticulum and granules of varying size. The cytoplasm is irregular in shape and in E.M. pictures shows the complex interdigitations that have been referred to by Follenius & Porte (1960) in the interstitial cells of the teleost Lebistes wticu- Zatus. As seen with the light microscope the cytoplasm shows a fine granulation which with Sudan dyes may give a more or less uniform background staining. However, staining tends to be more intense in the region immediately adjacent to the nucleus.…”

Section: Development Of the Secondary Sexual Charactersmentioning

confidence: 92%

The interstitial tissue of the testis of the River lamprey, Lampetra fluviatilis

Hardisty¹,

Rothwell²,

Steele³

1967

Journal of Zoology

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The cytology of the interstitial cells of the testis of the river lamprey has been studied from the autumn when the upstream migration begins up to the completion of sexual maturation in the following spring. In the early stages of spermatogenesis the interstitial tissue consists of isolated interlobular connective tissue cells. During the winter these appear to increase in numbers and form compact groups sometimes showing an acinar type of structure. In the earlier stages of the secretory cycle the cytoplasm contains small osmiophilic and sudanophilic granules, which appear to aggregate to form much larger lipid positive masses. In the later stages of spermatogenesis in early spring, the interstitium accumulates large areas of lipid and cholesterol positive droplets and in fixed material the cytoplasm shows extensive vacuolization. In the terminal stages the cells undergo complete disintegration. It seems probable that the stage when the interstitium shows masses of lipid and cholesterol positive droplets represents a phase of degeneration and that the secretion of hormone occurs in earlier stages. No convincing evidence has been obtained for the presence of the enzyme steroid‐3β‐ol‐dehydrogenase during the later stages of the secretory cycle. There is evidence that following the degeneration of the interstitium there may be a replacement of the secretory tissue by the transformation of residual connective tissue elements, which may then re‐enter the secretory phase. The changes in the interstitium are paralleled by hypertrophy and evidence of lipid metabolism in the modified fibroblasts of the lobular wall and this tissue may also be involved in the secretory activity of the testis.

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Cytologie fine des cellules interstitielles du testicule du poissonLebistes reticulatus R.

Cited by 32 publications

References 2 publications

Ultrastructural Studies of Germ Cell Development and the Functions of Leydig Cells and Sertoli Cells associated with Spermatogenesis in Kareius bicoloratus (Teleostei, Pleuronectiformes, Pleuronectidae)

Ultrastructural Studies of Germ Cell Development and the Functions of Leydig Cells and Sertoli Cells associated with Spermatogenesis in Kareius bicoloratus (Teleostei, Pleuronectiformes, Pleuronectidae)

The Normal Fine Structure of Opossum Testicular Interstitial Cells

The interstitial tissue of the testis of the River lamprey, Lampetra fluviatilis

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