Cytology and Biochemistry of Pathogenic Growth of Botrytis cinerea Pers. in Apple Fruit

Tronsmo, Anne Marte; Raa, Jan

doi:10.1111/j.1439-0434.1977.tb02860.x

Cited by 8 publications

(3 citation statements)

References 7 publications

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“…15 I was detected in certain of the initial extracts, including those of Monilinia fructigena in plum fruits. Similar observations on the apparent absence of PG were made by Skare et al (1975) in homogenates of cucumber infected with Cladosporium cucumerinum and by Tronsmo & Tronsmo (1977) in extracts of apple fruit infected by Botrytis cinerea.…”

Section: Introductionsupporting

confidence: 78%

Natural Inhibitors of Fungal Polygalacturonases in Infected Fruit Tissues

Fielding¹

1981

Microbiology

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Section: Introductionsupporting

confidence: 78%

Natural Inhibitors of Fungal Polygalacturonases in Infected Fruit Tissues

Fielding¹

1981

Microbiology

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“…Swelling of cell walls and severe disruption of bost tissues in advance of infection by B. cinerea bas been reported in TEM studies of leaves of lettuce (Cornford, Pitt & Wakley, 1985) and broad bean (Mansfield & Ricbardson, 1981) and in young tomato fruits (Rijkenberg et al, 1980) and tbese cbanges bave been attributed to the ability of B. cinerea to produce a multiplicity of cell wall degrading enzymes, proteases and lipases, together witb toxins (VerboefF, 1980). In tbe early stages of dry eye rot disease of apple, B. cinerea byphae are restricted to the middle lamella but during soft rot development in store tbe patbogen extends through tbe cellulose layers of tbe bost cell walls (Tronsmo, Tronsmo & Raa, 1977).…”

Section: I S C U S S I O Nmentioning

confidence: 99%

Use of cryo‐techniques with scanning electron microscopy to study infection of mature red raspberry fruits by Botrytis cinerea

Williamson¹,

Duncan²

1989

New Phytologist

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SUMMARYScanning electron microscopy (SEM) was used to study the infection of the mesocarp cells of ripe drupelets of the compound fruit of red raspberry (Rubus idaeus L.) hy Botrytis cinerea Pers.: Fr. 24 il after the IVtiit iiad heen wounded and inoculated vvith dry conidia. Frozen fully hydrated specimens showed that conidia had germinated in juice at the point of inoculation and in partialiy freeze-dried specimens hypiiae were visihle in some cells. However, structural detail was largely obscured by ice segregation zones in the closely packed large columnar, poyhedral cells of the fruit. These artifacts were removed in specimens whieh had been cryotrimmed and fully freeze-dried. In such specimens there were four distinct zones around the inoculation wound. Intercellular and intracellular hyphae were visible in the innermost area (zone 1); the walls of some infected cells were swollen, eroded and porous. In zone 2, an area of cells immediately beyond the hyphae, little structure was retained but in zone 3 cell walls were well preserved and showed lamellar or reticulate material in the intercellular spaces. The outer zone (zone 4) consisted of apparently healthy cells.

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“…During the study of pectic enzymes produced in fleshy plant organs by some pathogenic fungi, little or no endopolygalacturonase activity was detected, e.g. in apple fruits infected with S. fructigena (COLE and WOOD 1961b) or Botrytis cinerea (COLE andWOOD 1961b, TRONSMO andTRONSMO 1977), in pear fruits infected with B. cinerea or Dothiorella gregaria (ABU- GOUKH and LABAVITCH 1983) and in Capsicum fruits infected with Glomerella cingulata (BROWN and ADIKARAM 1982). Fruits have been shown to contain proteins which are capable of inhibiting certain pectinases, e.g.…”

mentioning

confidence: 99%

Relationship of Endopolygalacturonase Inhibitor Activity to the Rate of Fungal Rot Development in Apple Fruits

Brown¹

1984

Journal of Phytopathology

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An inhibitor extracted form the cell walls of apple fruits suppressed the activity of endopopygalacruronases (endo‐PGs) produced in vivo and in vitro by Nectria galligena, Phomopsis mali, Fusarium Lateritium and Glomerella cingulata but not the endo‐PGs produced by Penicillium expansum or Phytophtobora syringae. Of four apple cultivars tested Granny Smith tissue contained the highest levels of inhibitor and Cox's Orange Pippin contained the least. Linear rot expansion in the four apple cultivars inoculated with N. galligena was inversely related to inhibitor activity in the fruit tissue, rot development being slowest in Granny Smith fruits and most rapid in Cox's Orange Pippin fruits. Rot expansion in fruits inoculated with P. expansum bore no such relationship to inhibitor activity in the tissue Apple tissue maceration by the endo‐PGs from N. galligena, P. mali. F. lateritium and G. cingulata was similarly related to inhibitory activity in the fruit. The properties of the partially purified inhibitor were consistent with it being proteinaceous but the relative slowness with which it was hear inactivated and the presence of a small percentage of carbohydrate might indicate that it was a glycoprotein.

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Cytology and Biochemistry of Pathogenic Growth of Botrytis cinerea Pers. in Apple Fruit

Cited by 8 publications

References 7 publications

Natural Inhibitors of Fungal Polygalacturonases in Infected Fruit Tissues

Natural Inhibitors of Fungal Polygalacturonases in Infected Fruit Tissues

Use of cryo‐techniques with scanning electron microscopy to study infection of mature red raspberry fruits by Botrytis cinerea

Relationship of Endopolygalacturonase Inhibitor Activity to the Rate of Fungal Rot Development in Apple Fruits

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