Cytology and Cytochemistry of the Pericardial Gland Cells of Mytilus Edulis and their Lysosomal Responses to Injected Horseradish Peroxidase and Anthracene

Abstract

The functions and structure of the pericardial glands (Keber's organ) of bivalves were reviewed by White (1942). The cells of this gland have been considered to have an excretory role and Grobben (1887) suggested that the cells of the pericardial gland could be compared with the chloragogen cells of annelids.

Individual age and connective tissue lipofuscin in the hard clam Eurhomalea exalbida

“…Lipofuscin seems to be common in molluscs, as it has been reported in situ in various tissues and organs of several species such as the gastropods Maoricrypta monoxyla (Nelson and Morton, 1979) and Aplysia punctata (Taieb and Vicente, 1999) and the bivalves Mytilus galloprovincialis (Viarengo et al, 1987(Viarengo et al, , 1990Regoli, 1992;Sarasquete et al, 1992), Mytilus edulis (Moore et al, 1980;George et al, 1982;Lowe, 1988;Burbidge et al, 1994;Hole et al, 1995;Krishnakumar et al, 1994) and Perna viridis (Krishnakumar et al, 1990;Mathew and Damodaran, 1997). Only a few findings are reported from clams so far: In the alimentary tract epithelium, in gonadal connective tissue and in the renal epithelium of Mya arenaria and Mercenaria mercenaria (Brown and O'Toole, 1978); in the digestive gland of Ruditapes phillipinarum (Sarasquete et al, 1992) and of Sunetta scripta (Mathew and Damodaran, 1997); and in the kidney of Tridacna gigas (Reid et al, 1984).…”

“…We checked unstained thin sections (5 Am) for autofluorescent lipofuscin-like granules using a Zeiss III RS fluorescence microscope with Plan-Apochromat objective (25 Â ) and an HBO 50W AC super pressure mercury lamp with 365 nm as well as 450 nm excitation filters. The histochemical properties of candidate granules were assessed using the PAS -Alcian blue -hematoxylin (Moore et al, 1980) and the Sudan black B (see Bluhm et al, 2001a) techniques. The first of two subsequent sections was stained with the former, the second section with the latter.…”

“…Nelson and Morton, 1979;Moore et al, 1980;Mathew and Damodaran, 1997), crustaceans (e.g. Sheehy, 1989;Wahle et al, 1996;Medina et al, 2000;Bluhm et al, 2001a,b) and vertebrates (Eldred et al, 1982).…”

“…Donato and Sohal, 1978;Moore et al, 1980;Bassin et al, 1982; 0022-0981/02/$ -see front matter D 2002 Elsevier Science B.V. All rights reserved. PII: S 0 0 2 2 -0 9 8 1 ( 0 2 ) 0 0 2 4 0 -X Sheehy, 1989;Mathew and Damodaran, 1997;Sheehy et al, 1998;Bluhm et al, 2001a).…”

Comparative ultrastructural investigations of the podocyte in marine bivalve Pinctada radiata (Pteriidae) and freshwater bivalve Anodonta rubens (Unionidae)

“…These cells contain the prominent Golgi complexes with associated vesicles and large membrane-bound electron-dense granules. The vesicles may possibly be the primary lysosomes containing hydrolytic enzymes, which was detected as acid hydrolases by Moore et al ( 1980). The electron-dense granules may be the secondary lysosomes or the residual bodies containing materials from intracellular digestive processes.…”