Cytology and High-Risk Human Papillomavirus Test for Cervical Cancer Screening Assessment

Stübs, Frederik A.; Koch, Martin; Dietl, Anna; Adler, Werner; Geppert, Carol I.; Hartmann, Arndt; Knöll, Antje; Beckmann, Matthias W.; Mehlhorn, Grit; Schulmeyer, Carla E.; Gaß, Paul

doi:10.3390/diagnostics12071748

Cited by 9 publications

(16 citation statements)

References 36 publications

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“…The etiological role of HPV in the development of anogenital carcinomas has been widely studied and is well known [ 13 , 14 ]. However, the role of this virus in the development of non-small cell lung cancer is controversial and is currently under discussion.…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus in Non-Small Cell Lung Carcinoma: Assessing Virus Presence in Tumor and Normal Tissues and Its Clinical Relevance

et al. 2023

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The significance of the role of human papillomavirus (HPV) in the development of lung cancer remains an open question. The data from the literature do not provide conclusive evidence of HPV being involved in the pathogenesis of lung cancer. The aim of this work was to detect the presence of HPV infections with a high carcinogenic risk in patients with non-small cell lung cancer (NSCLC). Materials and methods: the study involved 274 patients with stage IIA–IIIB non-small cell lung cancer. We analyzed normal and tumor tissues as well as blood from each patient. DNA was extracted from patients’ specimens, and HPV detection and genotyping was carried out using commercially available kits by PCR. Results: HPV was detected in 12.7% of the patients (35/274 of all cases). We detected nine different types of human papillomavirus in the patients, namely, types 16, 18, 31, 35, 45, 51, 52, 56, and 59. The HPV-positive samples had a clinically insignificant viral load and were predominantly integrated. The relationship between the presence of HPV and its virological parameters and the clinical and pathological parameters of the patients was established. A metastatic-free survival analysis showed that all patients with HPV in the tumor tissue had a higher 5-year survival rate (94%) compared with the HPV-negative patients (78%). The result was not statistically significant (p = 0.08). Conclusions: data showing a 12.7% human papillomavirus representation among patients with non-small cell lung cancer were obtained. The presence/absence of a viral component in patients with lung cancer was a clinically significant parameter. HPV types 16, 18, and 56, which are the most oncogenic, were most often detected.

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Section: Discussionmentioning

confidence: 99%

Human Papillomavirus in Non-Small Cell Lung Carcinoma: Assessing Virus Presence in Tumor and Normal Tissues and Its Clinical Relevance

et al. 2023

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“…A new organized screening program was implemented in Germany in January 2020 [ 13 ]. Women between 20 and 34 years of age are continuing to have annual Pap smears, while women over the age of 34 receive a co-test comprising a Pap smear in combination with an hrHPV test every 3 years.…”

Section: Discussionmentioning

confidence: 99%

“…Women between 20 and 34 years of age are continuing to have annual Pap smears, while women over the age of 34 receive a co-test comprising a Pap smear in combination with an hrHPV test every 3 years. All women aged 20–65 are invited for testing by their health insurance providers every 5 years [ 13 , 31 , 32 , 33 , 34 , 35 , 36 ]. We therefore investigated the differences between the two different age groups.…”

Section: Discussionmentioning

confidence: 99%

“…There is considerable debate regarding the accuracy of Pap smears, which is reported to range between 53% and 78% [ 10 ]. The combination of colposcopy, cytology, and colposcopy-directed biopsy is the gold standard for diagnosing HSIL and cervical cancer [ 6 , 12 , 13 ]. Although cervical cancer screening is highly effective, the management of HSIL is a public health challenge [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Concordance Rate of Colposcopy in Detecting Cervical Intraepithelial Lesions

et al. 2022

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Background: The purpose of this research is to estimate the rate of concordance, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of colposcopy for high-grade squamous lesions and carcinomas (HSIL+). Methods: We conducted a retrospective study of colposcopies performed in the certified Dysplasia Unit in Erlangen between January 2015 and May 2022 (7.5 years). The colposcopic findings were correlated with biopsies obtained during examinations or surgery. Cases without histology were excluded. The primary outcome was the rate of concordance between the colposcopic and histological findings in relation to the type of transformation zone (TZ), examiner’s level of experience and age of the patients. Results: A total of 4778 colposcopies in 4001 women were analyzed. The rates of concordance for CIN I/LSIL, CIN II/HSIL, CIN III/HSIL, and carcinoma were 43.4%, 59.5%, 78.5%, and 53.9%, respectively. The rate of concordance was lowest for TZ3 and highest for colposcopists with more than 10 years’ experience. Conclusions: Colposcopy is an important, feasible, and effective method. Careful work-up needs to be performed for women with TZ3 who are over 35 years old, as they are at the highest risk of being misdiagnosed. The highest concordance for detecting HSIL+ was seen for colposcopists with >10 years’ experience.

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“…Bacterial and viral pelvic infections are significant diseases among young and adult females; they affect the female reproductive organs, including the vagina, cervix, womb, fallopian tubes, and ovaries. HPV is the most common viral infection of the reproductive tract and a leading sexually transmitted infection worldwide [ 16 , 17 ]. The vascular resistance (PI, RI) of the uterine arteries, ovarian arteries, and tubo-uterine arteries was low at the beginning of the acute phase of pelvic infection [ 18 , 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

Classification of Vascular Hotspots and Micro-Vessel Flow Velocity Waveforms in Low-Grade Squamous Intraepithelial Lesions and HPV Condyloma of the Cervix

Wu,

Chen,

et al. 2022

Diagnostics

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To assess hotspot micro-vessel flow velocity waveforms in human papillomavirus (HPV) cervical infections using transvaginal power Doppler ultrasound (TV-PDU) and to explore the associations of these sonographic parameters with HPV condyloma and low-grade squamous intraepithelial lesions (LSIL) of the cervix. A total of 39 patients with cervical HPV infections with abnormal cytology and colposcopy results (26 cases of LSIL; 13 cases of HPV condyloma) were enrolled to assess the vascular classification of the cervix and micro-vessel flow velocity using TV-PDU before treatment; 40 individuals with a pathologically normal cervix were used as the control group; seven parameters were measured, including vascular grading classification (Class I, Class II, and Class III), lowest pulsatility index (PI), resistance index (RI), peak systolic velocity (PS), end-diastolic velocity (ED), time average maximum velocity (TAMV), and the vascular index (VI = PS/ED). According to vascular classification, most LSILs were class I (69.2%, 18/26), followed by class II (26.9%, 7/26) and class III (3.8%, 1/26). Most HPV condylomas were class I (92.3%, 12/13), and one was class II (7.7%, 1/13). PI, RI, VI (p < 0.0001), and the PSs (p < 0.05) were significantly lower in these cases than in the controls. The ED and TAMV were not significantly different between the patients and controls (p = 0.4985 and p = 0.1564). No sonographic parameter was significantly different between LSIL and HPV condyloma. The mean PI, RI, and VI were significantly lower in LSIL than in the controls. For HPV condyloma, a PI of 1.07 had an 84.6% sensitivity, 85.0% specificity, and an AUC of 88.8%; for LSIL, a PI of 1.08 had a 100% sensitivity, 85% specificity, and an AUC of 94.2%; for HPV infection (HPV condyloma + LSIL), a PI of 1.08 had a 94.9% sensitivity, 85% specificity, and an AUC of 92.4%. Hotspot vascular classification and micro-vessel flow velocity waveforms may provide a potential practical method for the auxiliary diagnosis of cervical HPV infection. The PI may represent a valuable index for distinguishing the micro-vessel flow velocity waveforms in LSIL and HPV condyloma. Since the case numbers were limited in the current study, further validation is needed.

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Cytology and High-Risk Human Papillomavirus Test for Cervical Cancer Screening Assessment

Cited by 9 publications

References 36 publications

Human Papillomavirus in Non-Small Cell Lung Carcinoma: Assessing Virus Presence in Tumor and Normal Tissues and Its Clinical Relevance

Human Papillomavirus in Non-Small Cell Lung Carcinoma: Assessing Virus Presence in Tumor and Normal Tissues and Its Clinical Relevance

Concordance Rate of Colposcopy in Detecting Cervical Intraepithelial Lesions

Classification of Vascular Hotspots and Micro-Vessel Flow Velocity Waveforms in Low-Grade Squamous Intraepithelial Lesions and HPV Condyloma of the Cervix

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