Cytology and Kinetics of Spermatogenesis in the Rabbit

Swierstra, E. E.; Foote, R.H.

doi:10.1530/jrf.0.0050309

Cited by 122 publications

(71 citation statements)

References 13 publications

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“…The testes are the principal functional organs of the male reproductive system and are responsible for gametogenesis and steroidogenesis (6,20). On the basis of * This study is a summary of the Master's thesis of the first author.…”

Section: Introductionmentioning

confidence: 99%

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

BEYAZ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:This study was aimed at demonstrating the immunohistochemical expression of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis of the rabbit and at investigating the potential role of these molecules in testicular and epididymal physiology. The testes and epididymis of 15 male adult New Zealand rabbits constituted the material of the study. The streptavidinbiotin complex (ABC) immunohistochemical technique was used to determine the localization of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis. In the testes, immunoreactivity for VEGF and its receptors flt-1 and flk-1 was observed in the Sertoli cells and spermatogonia in the seminiferous tubules throughout all of the spermatogenic stages from I to VIII and in the Leydig cells of the interstitium. Furthermore, immunoreactivity was also observed in the acrosome of the spermatids in seminiferous tubules for VEGF at stages V, VI, VII and VIII and in the acrosome of the spermatids at stages VII and VIII and the blood vessels of the interstitium for flk-1. Positive immunoreactivity, ranging from strong to weak, was detected for VEGF, flt-1 and flk-1 in the cytoplasm of the principal, apical, basal and clear cells of the epithelium lining the epididymal initial segment and the caput, corpus and cauda epididymidis. The specific localization of the VEGF protein and its receptors flt-1 and flk-1 in the testes of the rabbit suggests that these molecules could have a functional role in several processes, including spermatogonial differentiation, spermiogenesis, acrosome development in spermatids, steroidogenesis in Leydig cells, epididymal sperm maturation, and the maintenance of the bloodepididymis barrier.Keywords: Epididymis, rabbit, testis, VEGF, VEGF receptors. Tavşan testis ve epididimislerinde vasküler endoteliyal büyüme faktörü ve reseptörlerinin immunohistokimyasal lokalizasyonuÖzet: Bu çalışmanın amacı, VEGF ve reseptörleri flt-1 ile flk-1'in tavşan testis ve epididimisindeki immunohistokimyasal ekspresyonlarını belirlemek ve bu moleküllerin testis ve epididimis fizyolojisindeki potansiyel rollerini araştırmaktır. Çalışmada materyal olarak, 15 adet sağlıklı, erkek Yeni Zellanda tavşanından elde edilen testis ve epididimisler kullanıldı. VEGF ve reseptörleri flt-1 ile flk-1'in testis ve epididimisdeki lokalizasyonlarının belirlenmesi amacıyla streptavidin biotin kompleks (ABC) immunohistokimyasal yöntemi kullanıldı. Testiste, I'den VIII'e kadarki tüm spermatojenik evrelerde seminifer tubüllerdeki Sertoli hücreleri ve spermatogonialarda ve intersitisyumda Leydig hücrelerinde VEGF ve reseptörleri flt-1 ile flk-1 için immunreaktivite gözlendi. Buna ilaveten, seminifer tubüllerde V., VI., VII. ve VIII. evrelerde VEGF için, VII. ve VIII. spermatojenik evrelerde ise flk-1 için spermatidlerin akrozomlarında ve intersitisyumda kan damarlarında immunreaktivite belirlendi. Epididimiste, başlangıç segmenti, kaput, korpus ve kauda epididimidis epitelindeki prensipal, apikal ve bazal hücreleri ile clear hücrelerinin sitoplazmaların...

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Section: Introductionmentioning

confidence: 99%

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

BEYAZ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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show abstract

“…Fig. 1 (Ortavant, 1958;Swierstra & Foote, 1963;Hochereau-de Reviers, 1970;Swierstra, Gebauer & Pickett, 1974). The prominent chromatin body in nuclei may cor¬ respond to the X and Y chromosomes (Matthey, 1950;Sachs, 1953 (Ortavant, 1958).…”

Section: Introductionmentioning

confidence: 99%

Spermatogenesis in Mature and Regressed Testes of the Vole (Microtus Agrestis)

Grocock¹,

Clarke²

1975

Reproduction

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“…The most advanced labelled germ cell type in specific stages of the cycle of the seminiferous epithelium (Swierstra & Foote, 1963) (Swierstra & Foote, 1963), these data provided a series of From these data and those from testicular autoradiographs, it can be con¬ cluded that about 31-4 days are required for the last 2-9 of the 4-0 to 4-5 cycles of the seminiferous epithelium which constitute spermatogenesis including spermatogonia renewal (Heller & Clermont, 1963 Table 7 the biological half-life of 3h-water in blood and seminal plasma they actually were collected; more than twenty-nine grains were associated with 13% and 7% of the spermatozoa in the first samples containing labelled spermatozoa.…”

Section: Resultsmentioning

confidence: 99%

“…Cytological aspects of rabbit spermatogenesis recently were discussed by Swierstra & Foote (1963). The present autoradiographic investigations pro¬ vided information on the duration of spermatogenesis and the transit of spermatozoa through the epididymides as well as the labelling pattern of ejaculated spermatozoa after thymidine-3H injection.…”

mentioning

confidence: 81%