Cytology and pollen morphology of Bougainvillea glabra ‘Elizabeth Angus’, a cultivar with low pollen fertility

Zhang, Wei; Wen-ling, Guan; Li, Yefang; Peng, Lvchun; Zhang, Lu; Meng, Jing; Wang, Jihua; Song, Jié

doi:10.1016/j.scienta.2022.111105

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…CMS is defective in producing functional pollen, where pollen abortion happened at various developmental stages ( Zhang et al., 2022 ). Previous studies have suggested that 38 species exhibited pollen abortion during anther development, 15.5% of them happened at the early stages of meiosis (13.5% in dicotyledonous plants, 2% in monocotyledonous plants), 57.5% happened at the tetrad stage (29% in dicotyledonous plants, 28.5% in monocotyledonous plants), and 27% happened at the microspore developmental stage (7.5% in dicotyledonous plants, 19.5% in monocotyledonous plants; Wang et al., 2015 ; Liu et al., 2018b ).…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Ge,

Ding,

Daniel

et al. 2023

Front. Plant Sci.

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IntroductionCytoplasmic male sterility (CMS) is an important tool for hybrid heterosis utilization. However, the underlying mechanisms still need to be discovered. An adequate supply of nutrients is necessary for anther development; pollen abortion would occur if the metabolism of carbohydrates were hampered.MethodsIn order to better understand the relationship between carbohydrate metabolism disorder and pollen abortion in S-CMS wheat, the submicroscopic structure of wheat anthers was observed using light microscopy and transmission electron microscopy; chloroplast proteome changes were explored by comparative proteomic analysis; sugar measuring and enzyme assays were performed; and the expression patterns of carbohydrate metabolism-related genes were studied using quantitative real-time PCR (qRT-PCR) method.ResultsThese results indicated that the anther and microspore in S-CMS wheat underwent serious structural damage, including premature tapetum degeneration, nutritional shortage, pollen wall defects, and pollen grain malformations. Furthermore, the number of chloroplasts in the anthers of S-CMS lines decreased significantly, causing abnormal carbohydrate metabolism, and disintegration of osmiophilic granules and thylakoids. Meanwhile, some proteins participating in the Calvin cycle and carbohydrate metabolism were abnormally expressed in the chloroplasts of the S-CMS lines, which might lead to chloroplast dysfunction. Additionally, several key enzymes and genes related to carbohydrate metabolism were significantly inhibited in S-CMS.DiscussionBased on these results, we proposed a carbohydrate metabolism pathway for anther abortion in S-type cytoplasmic male sterility, which would encourage further exploration of the pollen abortion mechanisms for CMS wheat.

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Section: Introductionmentioning

confidence: 99%

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Ge,

Ding,

Daniel

et al. 2023

Front. Plant Sci.

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Cytological observations in abortion formation and morphological character of pollen in seedless chestnut rose (Rosa sterilis)

Du,

Zhou,

Zhong

et al. 2023

Flora

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Morphological and metabolic changes in Changshan Huyou (Citrus aurantium) following natural tetraploidization

Huang,

Xu,

Wang

et al. 2024

Preprint

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Background Polyploids in citrus are generally used to improve crop varieties. Changshan Huyou (Citrus aurantium) is a native citrus species in China that is highly adaptable and has pharmaceutical value. However, the influence in Changshan Huyou following polyploidization remains unclear. Here we evaluated the phenotypic variations and metabolic alterations following autotetraploidization of Changshan Huyou seedlings and fruits and analyzed the associated transcriptomic changes. Result The tetraploid seedlings had rounder and thicker leaves, larger floral organs and fruits, and satisfactory viability of pollen grains and ovules. The tetraploid fruits accumulated lower levels of soluble solids but similar levels of organic acids. Metabolic profiling of three tissues of fruits revealed that most of 2064 differentially accumulated metabolites (DAMs), including flavonoids, lignans, and coumarins, were downregulated. In contrast, the upregulated DAMs mainly included alkaloids (clausine K and 2-(1-pentenyl)quinoline), amino acids (L-asparagine and L-ornithine), and terpenoids (deacetylnomilin and evodol) in tetraploid peels, as well as, flavonoids (neohesperidin and quercetin-5-O-β-D-glucoside) and organic acids (2-methylsuccinic acid and dimethylmalonic acid) in juice sacs. The upregulated genes were associated with phenylpropanoid biosynthesis, secondary metabolite biosynthesis, and the biosynthesis of various alkaloid pathways. The results of Pearson correlation analysis showed that the upregulated genes that encoded peroxidase and cytochrome P450 were closely related to a higher accumulation of amino acids and alkaloids in tetraploid peels, and neohesperidin and quercetin glucoside were increased by ferulate-5-hydroxylase, CYP450 81Q32, flavonoid 3'-monooxygenase, 4-coumarate–CoA ligase 1, and UDP-glucose flavonoid 3-O-glucosyltransferase, as well as, some transcription factors in tetraploid juice sacs. Conclusion The tetraploid Changshan Huyou investigated here may be used in breeding triploid citrus, especially to produce seedless varieties, and for pharmaceutical purposes in fruit processing, as they influence metabolites following polyploidization.

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Cytology and pollen morphology of Bougainvillea glabra ‘Elizabeth Angus’, a cultivar with low pollen fertility

Cited by 4 publications

References 24 publications

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Carbohydrate metabolism and cytology of S-type cytoplasmic male sterility in wheat

Cytological observations in abortion formation and morphological character of pollen in seedless chestnut rose (Rosa sterilis)

Morphological and metabolic changes in Changshan Huyou (Citrus aurantium) following natural tetraploidization

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