Cytology of the Adenohypophysis

Oordt, P. G. W. J. van

doi:10.1016/b978-0-12-455402-3.50007-3

Cited by 29 publications

(3 citation statements)

References 103 publications

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“…(2) extends the information of the histological features of the cell types in the distal lobe of Neoceratodus Hansen and Hansen 1994) by use of the classical pituitary staining concept established by van Oordt (1974); and (3) provides evidence that a distinctive population of distal lobe "basophils" of the pituitary of Neoceratodus contains a substance resembling mammalian TSH in its antigenic properties. Most, if not all, of these anti-TSH immunoreactive cells correspond to the type 2 basophils or basophil II cells previously described by Joss et al (1990).…”

Section: Discussionmentioning

confidence: 98%

Immunocytochemical localization and characterization of mammalian thyrotropin-like material in the pituitary of the Australian lungfish, Neoceratodus forsteri

Hansen

Hansen²

1998

Cell and Tissue Research

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The binding sites of polyclonal antisera raised against the beta-subunit of human thyroid-stimulating hormone (hTSHbeta), hTSH, and ovine TSH (oTSH) have been localized in the pituitary gland of the Australian lungfish, Neoceratodus forsteri, using light microscopy. Reactivity toward anti-TSH antiserum was demonstrated in a slightly elongated and irregularly-shaped distinct cell type forming clusters in the dorso-central and ventral regions of the distal lobe. Their granules react with alcian blue (AB), and with periodic acid-Schiff (PAS), and after AB-PAS-orange G they stain blue or purple. The specificity of the different antisera was established by liquid-phase absorptions and confirmed in positive and negative tissue control systems. Our observations confirm that dipnoan (Neoceratodus) TSH shares a number of antigenic determinants with those of mammalian TSHbeta and support the concept that mammalian TSHbeta, or part of it, was established early in evolution, and that dipnoans (Neoceratodus) as living sarcopterygians may have an ancestor in common with the early amphibians. The mapping and detailed description of TSH-like immunoreactive cells may furnish a background to facilitate current and future analysis of the ontogeny and time course of TSH production and release in Neoceratodus in relation to different physiological conditions.

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Section: Discussionmentioning

confidence: 98%

Immunocytochemical localization and characterization of mammalian thyrotropin-like material in the pituitary of the Australian lungfish, Neoceratodus forsteri

Hansen

Hansen²

1998

Cell and Tissue Research

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“…Following decapitation and removal of the lower jaw and the roof of the skull, the heads were immersion-fixed in Bouin's fluid for 72 h at 218C. The heads were subsequently decalcified in 5% nitric acid before the brains with the pituitaries attached were excised and washed for 24 h in running tap water, dehydrated in a graded series of ethanol, cleared in methyl benzoate followed by benzol, and embedded in paraffin (see van Oordt, 1974).…”

Section: Tissue Material Fixation and Staining Proceduresmentioning

confidence: 99%

Vascularization of the pituitary of the Australian lungfish and Neoceratodus forsteri

Hansen¹

1999

Acta Zoologica

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The vascularization of the brain and the pituitary region of the Australian lungfish, Neoceratodus forsteri is described from serial section reconstruction. The distal lobe has no direct arterial blood supply and receives blood solely from a pituitary portal system basically similar to that of other sarcopterygians. The primary capillary plexus of the median eminence receives its arterial blood from the infundibular arteries, which on their way distribute some small branches to the prechiasmatic region. The primary plexus also receives capillaries from the adjacent pial hypothalamic plexus. The primary capillary plexus of the median eminence comprises a rostral ‘uncovered’ and caudal ‘covered’ part which are not sharply delineated. Distinct portal vessels connect the ‘uncovered’ rostral part of the primary plexus with the secondary capillary plexus supplying the rostral subdivision of the pars distalis. The ‘covered’ caudal part of the primary plexus merges into the proximal subdivision of the pars distalis, apparently without formation of distinct portal vessels. The primary plexus has some connections with the plexus intermedius via a hypophysial stem capillary plexus. The plexus intermedius has a substantial arterial supply and gives off capillaries to the parenchyma of the pars intermedia. The adenohypophysis is drained into an unpaired hypophysial vein. The significance of the vascular pathways is discussed from comparative, functional, and evolutionary viewpoints.

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“…A final general characteristic is that the cells of the adenohypophysis are arranged in zones so that readily identifiable regions responsible for the production of ACTH or prolactin, for example, pars intermedia. A few studies have allocated specific hormones to identified cells (460,881,945).…”

Section: The Hypotha/amo-hypophyseal Complexmentioning

confidence: 99%

Endocrinology of the Vertebrates

Henderson

1997

Comprehensive Physiology

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Cytology of the Adenohypophysis

Cited by 29 publications

References 103 publications

Immunocytochemical localization and characterization of mammalian thyrotropin-like material in the pituitary of the Australian lungfish, Neoceratodus forsteri

Immunocytochemical localization and characterization of mammalian thyrotropin-like material in the pituitary of the Australian lungfish, Neoceratodus forsteri

Vascularization of the pituitary of the Australian lungfish and Neoceratodus forsteri

Endocrinology of the Vertebrates

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