Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Abstract

Impression cytology allowed collection of samples with maintained cytoarchitecture, while cytoplasmatic and nuclear details were often difficult to evaluate.

Conjunctival cytology assessment in dogs and cats. Sampling, diagnostic techniques and findings

“…It has been described that nylon-bristled cytobrushes for collection of conjunctival cytology specimens from veterinary patients, form more even monolayers and result in superior cell quality and yield, when compared to swab samples (Bauer et al, 1996;Willis et al, 1997;Perazzi et al, 2017). They do tend to be less cellular than those acquired by scraping but this technique is superior in safety and patient tolerance.…”

“…However, it will not offer clinically significant advantages over scrapings or cytological brush samples, both of which should collect a more satisfactory number of cells from deeper in the epithelium and the superficial stroma. This technique concerns cells that exfoliate with ease, therefore it is better suited when investigating superficial conjunctival disease (Bolzan et al, 2005;Perazzi et al, 2017). Besides using a clean glass slide, conjunctival imprints employing filter strips have been reported in dogs (Young, 2014).…”

Comparison of 3 corneal cytology collection methods for evaluating equine ulcerative keratitis: Cytobrush, kimura platinum spatula, and handle edge of scalpel blade

“…Corneal ulceration is common in the horse and is a frequent cause for veterinary examination . Collecting samples for cytological evaluation and microbial testing is routine practice in investigating the underlying cause or extent of the lesion . Cytologic examination is a safe, practical, and relatively inexpensive diagnostic tool that provides useful information for efficient assessment and guidance of therapy .…”

“…An ideal sample for cytologic evaluation is a well‐distributed monolayer of adequate cellularity that is representative of the lesion and causes the patient minimal irritation when obtained . The collection of cells for corneal cytology has been described using numerous tools and techniques in various species and can be divided into the use of sterile swab, brush, or tool for scraping the ocular surface …”

Cytology and the cell block method in diagnostic characterization of canine lymphadenopathy and in the immunophenotyping of nodal lymphoma

“…Bertazzolo et al report a high degree of agreement (kappa = 0.95) between four cytopathologists with varying levels of diagnostic training and experience in distinguishing adrenocortical tumours from pheochromocytoma . Perazzi et al described fair to perfect interobserver agreement between two cytopathologists in the cytological assessment of canine superficial ocular samples across nine descriptive categories and LeBlanc et al report partial to complete agreement in the evaluation of the quality of canine splenic cytology samples between two cytopathologists . To the authors' knowledge no previous published work has examined the diagnostic agreement among veterinary clinical pathologists in the assessment of canine lymph node samples aside from one study assessing interobserver agreement using the updated Kiel classification of canine lymphoma (kappa = 0.71) .…”