Cytology of Vicia Species. 5. Nuclear Chromatin Structure, Karyomorphological Analysis and DNA Content in Newly Discovered Relatives of Vicia faba L.: Vicia kalakhensis Khattab, Maxted et Bisby and Vicia eristalioides Maxted.

Cremonini, R.; Miotto, Doria; Ngu, M. Api; Tota, Donato; Pignone, Domenico; Blangiforti, Sebastiano; Venora, Gianfranco

doi:10.1508/cytologia.63.371

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…The diploid chromosome number of V. narbonensis L. was found to be 14 in all the studied accessions and this result agrees with the previous counts of Schäfer, 1973, Yamamoto, 1973, Raina et al, 1989, Cremonini et al, 1998, Perrino and Pignone, 1981, El-Bakatoushi and Ashour, 2009, Lamine, 2009. Fig.…”

Section: Resultssupporting

confidence: 91%

Pod, seed traits and cytotaxonomic studies of some Vicia narbonensis L. accessions ( Fabaceae )

El-Bok

Jabri

Ben-Brahim

et al. 2017

Saudi Journal of Biological Sciences

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This study aimed to characterize eight accessions of Vicia narbonensis L. originated from different Mediterranean countries. The cytology of these species is rarely known despite the fact of its great socio-economical and ecological interest in these arid and semi-arid zones. This work aimed mainly to characterize the karyotype, morphological pod and seed traits of the species. Karyotypes of all accessions were similar to a diploid number of 2n = 2x = 14. All the accessions have submetacentric chromosomes with a secondary constriction attached to the long arm of pair VII close to the centromere. Variation in chromosome size was observed; it ranged from 5.86 μm to 7.62 μm. Indices of karyotype asymmetry were calculated as the total form percentage (TF%) and symmetric indices (Syi) which ranged from 33.75% to 35.42% and from 51.01% to 54.85%, respectively. The predominance of submetacentric chromosomes indicated that the karyotype is symmetrical and can be considered as primitive. However, the analysis of quantitative parameters measured on pods and seeds showed a significant variation between accessions. A relationship between centromeric index and the pod beak length was found. Estimation of phenotypic diversity using the Shannon diversity index (H′) showed that the length, the seed color and the number of seeds per pod are the most polymorphic traits with respectively, H′ = 0.92, 0.80 and 0.83. Cluster analysis of karyological, pod and seed traits showed four groups of accessions. This clustering is partially due to the geographical origin of the studied accessions. The variation in chromosome size, pod and seed traits could offer potentially valuable genetic resources for the improvement of V. narbonensis which is considered as neglected and underutilized crop species (NUCS).

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Section: Resultssupporting

confidence: 91%

Pod, seed traits and cytotaxonomic studies of some Vicia narbonensis L. accessions ( Fabaceae )

El-Bok

Jabri

Ben-Brahim

et al. 2017

Saudi Journal of Biological Sciences

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“…La citometría de imagen es un método que combina las ventajas de la reacción de Feulgen con el análisis de imágenes obtenidas desde un microscopio óptico, permitiendo la estimación del tamaño del genoma en forma confiable y de bajo costo. El análisis de imágenes Feulgen fue utilizado en estudios cuantitativos de ADN y permitió determinar el tamaño del genoma en varias especies de plantas (Venora et al, 1995;Cremonini et al, 1998;Voglmayr & Greilhuber, 1998;Vilhar et al, 2001) incluyendo algunas especies de Solanaceae (Acosta et al, 2012), demostrando ser un método aceptado para la determinación del tamaño del genoma de la plantas (Praça-Fontes et al, 2011). agradEcImIEntos Los autores agradecen a las agencias Argentinas CONICET, ANPCyT -FONCyT, MINCyT -Córdoba, and SECyT -UNC por su apoyo financiero.…”

Section: Resultados Y Discusiónunclassified

Estimación del tamaño del genoma en especies de la tribu Cestreae (Solanaceae) mediante citometría de imagen.

Frossasco

Trenchi

Urdampilleta

2015

Bol. Soc. Argent. Bot.

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Los géneros Cestrum, Sessea y Vestia pertenecen a la tribu Cestreae y presentan varias características cromosómicas que los diferencian de otras especies de Solanaceae, como el número y tamaño cromosómico. El tamaño del genoma contribuiría a generar nuevas hipótesis sobre su evolución en los clados basales de Solanaceae. Para estimar el tamaño del genoma en especies de Cestreae fue utilizada la citometría de imagen de núcleos en interfase y telofase teñidos con la técnica de Feulgen. Los valores del tamaño del genoma en las especies analizadas de Cestreae varían entre 17,22 a 24,40 Gpb (2C), siendo los mayores tamaños de genoma de la familia. Además los resultados demuestran que la citometría de imagen es un método alternativo a la citometría de flujo que permite la determinación del tamaño del genoma en forma práctica y económica.

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“…In India species of the genus Vicia are distributed in the temperate zones of Western Himalaya and the Northern plains. Many species of the genus are cultivated for food and fodder (Cremonini et al 1998). Maxted et al (1991) have divided the genus Vicia into 2 subgenera: Vicilla and Vicia with 17 and 9 sections, respectively.…”

Section: Abstract Chromosome Number Cytomixis Meiotic Irregularitimentioning

confidence: 99%

Chromosome Number, Meiosis and Pollen Fertility in Vicia rigidula Royle and V. tenera Grah. from Cold Desert Regions of India

Kaur

Singhal

2010

CYTOLOGIA

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SummaryWe have recorded the meiotic chromosome counts in 2 species, Vicia rigidula (nϭ12) and V. tenera (nϭ7) from the cold desert region of Kinnaur district of Himachal Pradesh (India). V. rigidula which exists at tetraploid level (xϭ6) and depicts the phenomenon of cytomixis involving chromatin transfer in 6.52% of the observed pollen mother cells (PMCs) during different meiotic stages. 3.43-12.20% of PMCs also show non-synchronous disjunction of a few bivalents, laggards and chromatin bridges during anaphases/telophases, and micronuclei and included micronuclei in tetrads. Products of such aberrant PMCs result in the formation of sterile/unstained pollen grains and fertile/stained pollen grains of variable sizes. The meiotic course in V. tenera, which is diploid (xϭ7), depicts normal meiotic behaviour in the majority of the PMCs. Only a few PMCs (0.20-1.20%) depict some meiotic disturbances during anaphases resulting in the formation of 6.00-7.20% sterile/unstained pollen grains. Key words Chromosome number, Cytomixis, Meiotic irregularities, Vicia rigidula, V. tenera.The genus Vicia L., Tribe: Vicieae, Family: Fabaceae with 190 species (ILDIS 1999) is distributed in Europe, Asia and North America, extending to the temperate regions of South America and Tropical Africa (Maxted 1993). In India species of the genus Vicia are distributed in the temperate zones of Western Himalaya and the Northern plains. Many species of the genus are cultivated for food and fodder (Cremonini et al. 1998). Maxted et al. (1991) have divided the genus Vicia into 2 subgenera: Vicilla and Vicia with 17 and 9 sections, respectively. Most of the species of the genus are annuals, but a few of them are perennials. V. rigidula Royle is a perennial subglabrous climber with 2-3 inch long leaves ending in intricate tendrils and with golden yellow flowers appearing during June to September. The species is found in the temperate regions of Western Himalaya between altitudes of 1500-3000 m. V. tenera Grah. grows as a perennial subglabrous climber with pinnate leaves and pinkish-purple flowers which appear during May-July. The species is distributed at elevations of 1800-2600 m in moist shady places.Perusal of chromosomal data on the basis of information gathered from Index to Plant Chromosome Numbers and other authors (Srivastava 1963, Raina and Bisht 1988, Kumari and Bir 1990, Nikiforova 1990, Maxted et al. 1991, Venora et al. 1991, Akpinar and Bilaloglu 1997, Bisht et al. 1998, Kamel 1999, Bagci and Sahin 2000, Kotseruba et al. 2000, Rahiminejad et al. 2000, Tabur et al. 2000, 2002 reveals that a large number of species of the genus Vicia are yet to be known chromosomally, especially those from the high altitudinal regions and cold deserts of Indian Himalayas. We have worked out the chromosome counts in V. rigidula and V. tenera collected in Kinnaur district of Himachal Pradesh. Simultareously, we have also recorded our observations on male meiotic course, pollen sterility and pollen grain size in these species.

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Cytology of Vicia Species. 5. Nuclear Chromatin Structure, Karyomorphological Analysis and DNA Content in Newly Discovered Relatives of Vicia faba L.: Vicia kalakhensis Khattab, Maxted et Bisby and Vicia eristalioides Maxted.

Abstract: SummaryThree species of the Vicia narbonensis complex, namely V. narbonensis L., V. kalaken-

Cited by 14 publications

References 22 publications

Pod, seed traits and cytotaxonomic studies of some Vicia narbonensis L. accessions ( Fabaceae )

Pod, seed traits and cytotaxonomic studies of some Vicia narbonensis L. accessions ( Fabaceae )

Estimación del tamaño del genoma en especies de la tribu Cestreae (Solanaceae) mediante citometría de imagen.

Chromosome Number, Meiosis and Pollen Fertility in Vicia rigidula Royle and V. tenera Grah. from Cold Desert Regions of India

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