Cytology, pollen stainability and yield in desynaptic autotetraploid Capsicum.

Singh, R. N.; Roy, S. K.

doi:10.1508/cytologia.49.797

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Seeds were tested for their diploid character and normal feature of meiosis. The colchicine solution was applied to the apical buds of the plant (Singh and Roy, 1984).…”

Section: Methodsmentioning

confidence: 99%

Meiotic Behaviour and Pollen Fertility in Colchiploid Zea mays L.

.¹,

Ahmad²

1998

Pakistan J. of Biological Sciences

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Colchicine at various concentration was given to the apical buds of maize (Zea mays L.). Plants, in order to induce tetraploidy for meiotic studies. Highly irregular meiosis in the C 1 generation was revealed. These meiotic abnormalities increased pollen sterility which lead to a lower seed setting. In the C 2 generation the occurrence of bivalents has increased and that of multivalents has decreased. Restoration of meiotic regularities, pollen fertility.

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“…Seeds were tested for their diploid character and normal feature of meiosis. The colchicine solution was applied to the apical buds of the plant (Singh and Roy, 1984).…”

Section: Methodsmentioning

confidence: 99%

Meiotic Behaviour and Pollen Fertility in Colchiploid Zea mays L.

.¹,

Ahmad²

1998

Pakistan J. of Biological Sciences

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“…Tested for their diploid character and normal feature of meiosis. The col chicine treatment was applied on apical portion of the plant (See Singh and Roy 1984).…”

Section: Methodsmentioning

confidence: 99%

Induced colchiploidy in Petunia hybrida Hort. Morphocytogenetical studies.

Singh

Roy

1988

CYTOLOGIA

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Increase in the productivity or improvement in the quality of a plant by the use of fertilizers has limitations while these aspects can be achieved with greater assertion and to a long range advantage by genetical means. Of these the application of the methods of selection, hybridi zation and polyploidy had been most fruitful in bringing about all round betterment in many cases of agricultural, horticultural or even wild plants. Petunia hybrida Hort. is a common ornamental plants grown for its beautiful flowers which range in colur from white to deep red or purple (Bailey 1958). With the increase of the ploidy level all or most of these can be achieved although they may be associated with some disadvantage such as poor seed setting. However, reduce seed setting or such disabilities may assume lesser importance in this orna mental because the plant has too many seeds per capsule. In the present experiment, P. hybrida (pink) has been selected to induce autoploidy to observe the following objectives: Lengthening of flowering period, increasing the colour diversity and improving the size and keeping quality of flowers. Materials and methodsThe seeds of Petunia hybrida Hort. (pink) Vilm. has been utilized in the present study and collected from the plants grown for several successive generations in the botanical graden of the university. Tested for their diploid character and normal feature of meiosis. The col chicine treatment was applied on apical portion of the plant (See Singh and Roy 1984).After treatment, the dimension of the plant parts changed and these were documented after measuring the plant height, internodal length, leaf (L/W), stomatal size, flower size and seed setting (Average of 10 plants in each case).For meiotic studies, flower buds of different treatment plants were fixed in acetic-alcohol (1:3) with a few drop of ferric chloride in it to improve staining. Squashes were made per manent by t-butyl alcohol schedule.Heating the pollen grains in a drop of 1% acetocarmine solution, those which suitably stained were taken as fertile and the empty shrunken ones as sterile. Fertility percentage was scored on the average of 200 pollen grains. ObservationsVarious concentration of colchicine (0.20-0.50%) was applied on the apical bud and the 0.25% concentration was found effective in inducing the maximum number of tetraploid plants. Out of thirty plants were treated with above solution, 63.33% plants were survived and only 30% turned out as tetraploid (Table 1). Seeds obtained from induced tetraploids by selfing were sown in the field and plants of C2 generation were also found to be tetraploid on being cytologically investigated. Observations on induced autotetraploidy in P. hybrida was extended over first (C1) and second (C2) generation using suitable parameters.

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Meiotic behaviour and pollen fertiity in C1 and C2 generation of autotetraploid Phlox.

Singh

1991

CYTOLOGIA

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Induced polyploidy is known to enhance the production potential of plants where veg etative organs and their biomass constitute the economic product (Lavania 1986). It is well known that experimentally produced autotetraploids show a reduced fertility as related to their diploid initial lines. This is true for the pollen as well as the seed fertility. With regard to the seed fertility, the situation is considerably worse. In the family Polemoniaceae, genus Phlox (2n=14) has mainly horticultural plants in which ploidy was induced to increase flower size as well as seed out put, with a hope that enough variability could be created by the induc tion of polyploidy and selection from segregation generation which would yield better genotype for the improvement of the plant. In the present study chromosome pairing behaviour and pollen fertility were investigated up to second (C2) generation. Materials and methodsThe seeds of Phlox drummondii Hook., family polemoniaceae, were collected from the healthy plants of botanical garden of the University. Seeds were tested for their diploid character and normal feature of meiosis. The colchicine solution was applied to the apical buds of the plant (see Singh and Roy 1984). First generation (C1)The effects induced by colchicine were measured by following parameters:Meiotic abnormalities For meiotic studies, flower buds were fixed in acetic-alcohol (1:3) with a few drop of ferric chloride in it to facilitate staining.Squashes were made permanent by t-butyl alcohol schedule and microphotographs were taken for every important observation and present whenever possible. Pollen fertilityHeating the pollen grains in a drop of 1% aceto-carmine solution, those which suitably stained were observed as fertile and the empty, shrunken ones as sterile. Fertility percentage was scored on the average of 200 pollen grains. Second generation (C2)The seeds were collected from C1 generation and sown for the study of C2 generation.Meiotic abnormalities Second generation meiocytes were examined on the lines of first generation (C1).

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Cytology, pollen stainability and yield in desynaptic autotetraploid Capsicum.

Cited by 3 publications

References 20 publications

Meiotic Behaviour and Pollen Fertility in Colchiploid Zea mays L.

Meiotic Behaviour and Pollen Fertility in Colchiploid Zea mays L.

Induced colchiploidy in Petunia hybrida Hort. Morphocytogenetical studies.

Meiotic behaviour and pollen fertiity in C1 and C2 generation of autotetraploid Phlox.

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