Cytomegalovirus Antibodies in Breast Milk and Sera of Mother-infant Pairs

Kassim, Olakunle O.; Afolabi, Oladapo O.; Ako-Nai, Kwashie Ajibade; Torimiro, S. E. A.; Littleton, G. K.; Oke, O O; Grissom, F. E.

doi:10.1093/tropej/33.2.75

Cited by 13 publications

(14 citation statements)

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“…Furthermore, neither sIgA-nor IgG-opsonized bacteria stimulated chemiluminescence. This indicates that optimal chemiluminescence is dependent on the presence of bound C3 fragments, which are known to be present in low concentrations (0.1-0.16 mg/ml) in breast milk (3,5,26). Thus a synergistic interaction between complement and Fc receptors is necessary for the generation of a maximal oxidative response.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial properties of breast milk: Requirements for surface phagocytosis and chemiluminescence

Avery

Gordon

1991

Eur. J. Clin. Microbiol. Infect. Dis.

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The opsonic components of breast milk responsible for phagocytosis of surface-adherent Staphylococcus aureus by human polymorphonuclear leukocytes were investigated. There was significantly greater phagocytosis of bacteria pre-opsonized with 100% breast milk than of unopsonized bacteria (p less than 0.001). Heat inactivation of breast milk had no effect on surface phagocytosis, indicating that phagocytosis is independent of complement. The predominant immunoglobulin in breast milk, secretory immunoglobulin A (IgA), did not promote phagocytosis. In contrast, IgG, which is present in very low amounts in breast milk (0.05 mg/ml), was as opsonic as 100% breast milk, suggesting that this is the major opsonin. An oxidative burst as measured by chemiluminescence was observed during phagocytosis of bacteria pre-opsonized with 100% breast milk. Heat inactivation of breast milk reduced the chemiluminescence response to the level of control. Neither secretory IgA nor IgG stimulated a polymorphonuclear leukocyte chemiluminescence response to surface-adherent bacteria. These experiments indicate that IgG is the principal component of breast milk responsible for surface phagocytosis but that complement is required for the generation of chemiluminescence and thus may be essential for intracellular killing of bacteria. Secretory IgA, despite its abundance in breast milk, has no effect on surface phagocytosis or neutrophil chemiluminescence.

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Section: Discussionmentioning

confidence: 99%

Antibacterial properties of breast milk: Requirements for surface phagocytosis and chemiluminescence

Avery

Gordon

1991

Eur. J. Clin. Microbiol. Infect. Dis.

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“…According to Abu-Madi et al 25 , most of the children and adolescents in Qatar, Arabian Gulf, appear to have been exposed to CMV with seroprevalences of 79.0% in the 2 to 10-year and 91.0% in the 11 to 20-year age groups 25 . Kassim et al 26 reported that 91.0% of 33 mothers were seropositive for CMV compared to 33.0% of their infants 17 .…”

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confidence: 99%

High seropositivity of IgG and IgM antibodies against cytomegalovirus (CMV) among HIV-1 seropositive patients in Ilorin, Nigeria

Fowotade¹,

Okonko²,

Agbede

et al. 2015

Afr H. Sci.

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Background: Human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) is a major public health problem in sub-saharan Africa. Cytomegalovirus (CMV) has been reported to enhance HIV replication and accelerate the progression of HIV infection to AIDS. Objective: This study reports on the high seropositivity of immunoglobulin (Ig) G and M antibodies against CMV and the risk factors for CMV infection among HIV/AIDS patients in Ilorin, Nigeria. Method: A total of 180 consented HIV-1 seropositive patients (age-range 16-56 years; 108 females and 72 males) were consecutively recruited. Socio-demographic/behavioral data and 5 ml blood samples were collected from each patient. Plasma of each sample was assayed for anti-CMV IgG/IgM using a CMV IgG and IgM Enzyme Linked ImmunoSorbent Assay (ELISA) kit. Results: Twenty (11.1%) of the 180 HIV-1 seropositive subjects were positive for anti-CMV IgM antibody while 169(93.9%) were positive for anti-CMV IgG antibody. Age, marital status, number of sexual partners, CD4 cells counts and previous history of blood transfusion were the main correlates of CMV seropositivity among these patients. However, occupation, sex, highly active antiretroviral therapy (HAART) were not statistically associated with CMV seropositivity in this study. Conclusion:This study has shown that greater percentages of HIV-1 seropositive patients had active CMV infection. It has further shown that CMV is hyperendemic in HIV-1 seropositive patients in Ilorin, Nigeria.

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“…In Nigeria, a study conducted in 2008, reported a prevalence of 45.0% and 33% IgM antibodies among breastfeeding mothers and their infants [38]. Similarly, a study carried out in Kafanchan among pregnant women reveals prevalence of 10.5% of IgM specific antibodies [40].…”

Section: Discussionmentioning

confidence: 99%

“…In Nigeria Cytomegalovirus has been reported as 45% and 33% in breast milk, 86.15% (IgG) among expected mothers, 77.1% (IgG) among primigravid women in Bida, 97.2% (IgG) among pregnant women in Lagos, and 10.5% (IgM) among pregnant women in Kafanchan Kaduna State [30,38,39,40]. According to Spring, 1998 50% of babies born with symptoms of CMV infections and 15% of babies born with asymptomatic CMV infection will develop some degree of hearing loss [8].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria

Usman¹

2017

AJLM

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Abstract:Cytomegalovirus cause viral congenital infection at any stage of pregnancy leading to congenital defects, which can either be primary or recurrent and hearing loss is one of the common congenital CMV infection occurring in 10-15% of infected children. This study, therefore examined the seroprevalence of cytomegalovirus among paediatric patients with hearing loss attending National Ear Care Centre Kaduna northwest Nigeria. A total of 77 samples was collected from patients aged between 0-13 years, diagnosed of hearing impairment and screened for Cytomegalovirus IgG and IgM specific antibodies using ELISA method at National Ear Care Centre Kaduna State North West Nigeria. Among the 77 (100%) patients screened, 35 (45.5%) were male, while 42 (54.5%) were female with mean age (2.04). The study reveavled that, of the total numbers screened, 62 (80.5%) were IgG specific antibodies positive, while 15 (19.5%) were IgG specific antibodies negative, and none of the patient were positive for IgM specific antibodies. the result of patients based on their gender status show male patients 21 (60%) were positive while 14 (40%) were negative for CMV IgG specific antibodies. The female is 33 (78.5%) positive and 9 (21.4%) negative for CMV IgG specific antibodies. The findings hearing impairment is associated with cytomegalosvirus showing statistically significant in IgG specific antibodies (P<0.05), while in IgM it is not statistically significant. Therefore CMV infection is prevalent among patients with Hearing impairment and affect the social economic and political status of the patients.

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Cytomegalovirus Antibodies in Breast Milk and Sera of Mother-infant Pairs

Cited by 13 publications

References 0 publications

Antibacterial properties of breast milk: Requirements for surface phagocytosis and chemiluminescence

Antibacterial properties of breast milk: Requirements for surface phagocytosis and chemiluminescence

High seropositivity of IgG and IgM antibodies against cytomegalovirus (CMV) among HIV-1 seropositive patients in Ilorin, Nigeria

Seroprevalence of Cytomegalovirus Among Paediatric Patients with Hearing Loss Attending National Ear Care Centre Kaduna Northwest Nigeria

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