Cytomegalovirus-Antigenemia-Positive and Polymerase-Chain-Reaction-Negative Transplant Patient

Hernandez, Amanda K.; Emmons, Jacqueline; Wimmer, Daniel; Payne, Deborah

doi:10.1309/nhkbj8ufm8hkyjrx

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…A 116 bp gene region of the envelope glycoprotein B (gpUL55) was used for the identification of CMV genome by the TaqMan real-time PCR method. The primer and probe sequences were: CMV-r ; 5'-AAGTACCCCTATCGCGTGTG-3', CMV-f; 5'-ATGATGCCCTCRTCCARGTC -3', CMV-P ; 5'-FAM-TGGCCCAGGGTACGGATCTTATTCG-BHQ1-3' (18). The realtime PCR amplification program was performed at 94°C for 10 minutes, followed by denaturation at 94°C for 10 seconds, and annealing and extension at 60°C for one minute (in 50 cycles).…”

Section: Screeningmentioning

confidence: 99%

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Aghdam

Nadji

Khoddami

et al. 2020

Arch Pediatr Infect Dis

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Background: Identifying etiologic factors contributing to Wilms tumor (WT) is necessary for its prevention and treatment. Oncogenic viruses cause nearly 20% of all human cancers. Although trials on preventing virus-caused cancers are complex and difficult, but they are not impossible to conduct. Human Cytomegalovirus (HCMV) and human herpes virus-6 (HHV6) can cause different types of cancers. Objectives: The current study aimed to investigate whether HCMV and HHV6-DNA are present in two patients with WT. This is the first study of this kind in Iran. Methods: This is a cross-sectional study performed on patients with kidney disorders who were referring to Mofid Pediatrics Hospital, Tehran (Iran), during 2010-16. In total, 98 kidney samples (49 patients with WT and 49 normal kidneys (autopsy) and kidneys with benign noninfectious lesions) were investigated to identify HCMV and HHV6-DNA. Qualitative Polymerase Chain reaction (PCR) method and nested polymerase chain reaction (nested-PCR) technique were used to identify HCMV and HHV6, respectively. Results: No significant difference was found between WT patients and controls concerning the HCMV or HHV6. Conclusions: Based on the findings, it can be concluded that there is no association between these viruses and WT.

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Section: Screeningmentioning

confidence: 99%

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Aghdam

Nadji

Khoddami

et al. 2020

Arch Pediatr Infect Dis

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Cytomegalovirus and Langerhans Cell Histiocytosis: Is There a Link?

et al. 2016

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Background:Langerhans cell histiocytosis is a rare proliferative histiocytic disease of unknown etiology. Histologically, it is characterized by granuloma-like proliferation of Langerhans-type dendritic cells derived from bone marrow. Many investigators have suggested the possible role of viruses such as Epstein-Barr virus, human herpesvirus-6 (HHV-6), herpes simplex virus (HSV) types 1 and 2, and Cytomegalovirus in the pathogenesis of Langerhans cell histiocytosis.Objectives:In this study, we have investigated the presence of Cytomegalovirus in Langerhans cell histiocytosis in Iranian children.Patients and Methods:In this retrospective study, we have investigated the presence of Cytomegalovirus DNA expression, using paraffin-embedded tissue samples of 30 patients with Langerhans cell histiocytosis and 30 age and site-matched controls by qualitative Polymerase Chain Reaction (PCR) method.Results:No significant difference in prevalence of Cytomegalovirus presence between patients and controls was found. Cytomegalovirus was found by qualitative PCR in only 2 (6.66%) out of 30 patients and in 1 (3.3%) of 30 control samples with a P value of 1 (1.00 > 0.05) using chi-square test with OR: 2.07; 95% CI of OR: 0.18 - 24.15.Conclusions:Our findings do not support the hypothesis of a possible role for Cytomegalovirus in the pathogenesis of Langerhans cell histiocytosis.

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Cytomegalovirus-Antigenemia-Positive and Polymerase-Chain-Reaction-Negative Transplant Patient

Cited by 2 publications

References 3 publications

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Human Cytomegalovirus and Human Herpesvirus-6 and Wilms Tumor: Is There a Link?

Cytomegalovirus and Langerhans Cell Histiocytosis: Is There a Link?

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