Cytomegalovirus Genotypes gB1 and gH1 Are the Most Predominant Genotypes among Renal Transplant Recipients in Kuwait

Madi, Nada; Al‐Nakib, W.; Pacsa, A.S.; Saeed, Talha Bin

doi:10.1016/j.transproceed.2011.02.053

Cited by 13 publications

(14 citation statements)

References 12 publications

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“…Similar findings were reported in previous studies that demonstrated that the gH2 variant was the most prevalent genotype observed in 61-62% of newborns with congenital CMV infection [Ross et al, 2011;de Vries et al, 2012]. Conversely, the gH1 genotype was predominant in a Japanese population of infants infected congenitally [Yan et al, 2008] and renal transplant recipients in Kuwait [Madi et al, 2011]. The pathogenesis of hearing loss in children with congenital CMV infection is poorly understood and may occur in 22-65% of children with symptomatic CMV infection and in 6-23% of those who are asymptomatic at birth [Fowler and Boppana, 2006].…”

Section: Discussionsupporting

confidence: 91%

“…Similar findings were reported in previous studies that demonstrated that the gH2 variant was the most prevalent genotype observed in 61–62% of newborns with congenital CMV infection [Ross et al, ; de Vries et al, ]. Conversely, the gH1 genotype was predominant in a Japanese population of infants infected congenitally [Yan et al, ] and renal transplant recipients in Kuwait [Madi et al, ].…”

Section: Discussionsupporting

confidence: 89%

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Cytomegalovirus glycoprotein H genotype distribution and the relationship with hearing loss in children

Paradowska

Jabłońska

Studzińska

et al. 2014

Journal of Medical Virology

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Cytomegalovirus (CMV) is a leading cause of congenital infection and a leading infectious cause of hearing loss in children. The ORF UL75 gene encodes envelope glycoprotein H (gH), which is essential for CMV entry into host cells and the target of the immune response in humans. However, the distribution of gH variants and the relationship between the viral genotype, viral load, and sequelae in children infected with CMV is debated. The UL75 genetic variation of CMV isolates from 42 newborns infected congenitally with CMV and 93 infants with postnatal or unproven congenital CMV infection was analyzed. Genotyping was performed by analysis of PCR-amplified fragments, and the viral load was measured by quantitative real-time PCR. There were no differences in the distribution of gH genotypes in the children infected congenitally and postnatally. Mixed-genotype infections with both gH1 and gH2 variants were detected in approximately 25% of the examined patients. No relationship between UL75 gene polymorphisms and the symptoms at birth was observed. The results suggest that the infection with gH2 genotype diminishes the risk of hearing loss in children (P = 0.010). In addition, sensorineural hearing loss was associated with CMV gH1 genotype infection in infants (P = 0.032) and a high viral load in urine (P = 0.005). In conclusion, it was found that the gH genotype does not predict clinical sequelae in newborn infants following congenital CMV infection. However, these results suggest that the gH genotype might be associated with hearing loss in children.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Cytomegalovirus glycoprotein H genotype distribution and the relationship with hearing loss in children

Paradowska

Jabłońska

Studzińska

et al. 2014

Journal of Medical Virology

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“…Multiplex PCR revealed that gB-1 is the most prevalent genotype in both non-IBC and IBC carcinoma tissues with no significant difference between both patient groups. The present result is in agreement with previous studies, which reported that gB-1 had the highest incidence in renal transplant recipient HCMV-infected patients in Kuwait (57) and among children who live at the Phayathai Babies’ Home in Non-thaburi, Thailand (58). The predominance of the gB-1 genotype in HCMV-infected patients was also recorded in immunocompromised patients undergoing organ transplants (15) and congenitally infected neonates (59, 60) in a Chinese population.…”

Section: Discussionsupporting

confidence: 94%

Inflammatory Breast Cancer: High Incidence of Detection of Mixed Human Cytomegalovirus Genotypes Associated with Disease Pathogenesis

et al. 2014

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Inflammatory breast cancer (IBC) is a highly metastatic, aggressive, and fatal form of breast cancer. Patients presenting with IBC are characterized by a high number of axillary lymph node metastases. Recently, we found that IBC carcinoma tissues contain significantly higher levels of human cytomegalovirus (HCMV) DNA compared to other breast cancer tissues that may regulate cell signaling pathways. In fact, HCMV pathogenesis and clinical outcome can be statistically associated with multiple HCMV genotypes within IBC. Thus, in the present study, we established the incidence and types of HCMV genotypes present in carcinoma tissues of infected non-IBC versus IBC patients. We also assessed the correlation between detection of mixed genotypes of HCMV and disease progression. Genotyping of HCMV in carcinoma tissues revealed that glycoprotein B (gB)-1 and glycoprotein N (gN)-1 were the most prevalent HCMV genotypes in both non-IBC and IBC patients with no significant difference between patients groups. IBC carcinoma tissues, however, showed statistically significant higher incidence of detection of the gN-3b genotype compared to non-IBC patients. The incidence of detection of mixed genotypes of gB showed that gB-1 + gB-3 was statistically significantly higher in IBC than non-IBC patients. Similarly, the incidence of detection of mixed genotypes of gN showed that gN-1 + gN-3b and gN-3 + gN-4b/c were statistically significant higher in the carcinoma tissues of IBC than non-IBC. Mixed presence of different HCMV genotypes was found to be significantly correlated with the number of metastatic lymph nodes in non-IBC but not in IBC patients. In IBC, detection of mixed HCMV different genotypes significantly correlates with lymphovascular invasion and formation of dermal lymphatic emboli, which was not found in non-IBC patients.

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“…Different HCMV gH genotypes may be associated with disease severity (11)(12)(13)(14). Using multiplex PCR plus restriction fragment length polymorphism to discriminate between HCMV gH genotypes is complicated when using clinical samples.…”

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confidence: 99%

Rapid and Sensitive Identification of Glycoprotein H Genotypes in Clinical Human Cytomegalovirus Samples

Tao

Zhang

et al. 2015

Jpn J Infect Dis

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SUMMARY: Human cytomegalovirus (HCMV) is a common pathogen that causes persistent infections in immune deficient patients and results in significant morbidity and mortality, particularly among transplant recipients and children. Different HCMV glycoprotein H (gH) genotypes may cause different diseases and affect the severity of these diseases. To develop a sensitive quantitative real-time PCR assay that could rapidly distinguish between two HCMV gH genotypes, primers were designed to target the conserved region of the gH gene. gH1 and gH2 probes were designed to target the two variable regions. Standard HCMV strains (AD169 and TOWNE) and 203 clinical urine samples from HCMV infected children were used for the present study. Based on the primer-probe set used to detect the target gH gene segment of HCMV, our quantitative real-time PCR assay specifically discriminated between HCMV gH1 and gH2 with a detection limit of approximately 10 2 viral copies/ml. Among the 203 clinical urine samples tested, 145 were gH1 positive, 56 were gH2 positive, and 2 were positive for both. Thus, we developed a gH gene-based real time-PCR method that could rapidly, stably, and specifically distinguish between two HCMV gH genotypes. We found HCMV gH1 to be common among children examined in Zhejiang, China.

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Cytomegalovirus Genotypes gB1 and gH1 Are the Most Predominant Genotypes among Renal Transplant Recipients in Kuwait

Cited by 13 publications

References 12 publications

Cytomegalovirus glycoprotein H genotype distribution and the relationship with hearing loss in children

Cytomegalovirus glycoprotein H genotype distribution and the relationship with hearing loss in children

Inflammatory Breast Cancer: High Incidence of Detection of Mixed Human Cytomegalovirus Genotypes Associated with Disease Pathogenesis

Rapid and Sensitive Identification of Glycoprotein H Genotypes in Clinical Human Cytomegalovirus Samples

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