Cytomegalovirus Identification in Blood and Urine of Newborns by Nested PCR

Abstract

Cytomegalovirus detection in urine was slightly more efficient than in blood, and showed better sensitivity than in serological analysis ( < 0.01) therefore, boiled urine may be a better and easier specimen tool for CMV diagnosis in neonatal infection. The findings of the present research suggest that patients admitted to the NICU, especially premature infants, whose laboratory results are not compatible with bacterial infection, and exhibiting signs suggestive of CMV infection should have PCR done on urine for confirmation.

Frequent pUL27 Variations in HIV-Infected Patients

“…We studied 20 CMV-positive samples provided by our previous study [13]. Briefly, DNA of 2,000 samples from neonates with congenital disease and 180 HIV-infected patients have been extracted by boiling procedure and checked for CMV infection by using polymerase chain reaction (PCR) [14]. …”