Cytomegalovirus Infection: Perinatal Implications

Damato, Elizabeth G.; Winnen, Caitlin W.

doi:10.1111/j.1552-6909.2002.tb00026.x

Cited by 40 publications

(26 citation statements)

References 32 publications

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“…This is due to the prevalence of perinatal infection because of the growth of the infection in women of childbearing age, as well as opportunities (4-10%) of perinatal infection from mother to infant [4,5]. Among the pregnant women, they account for 42.6 of 94.5%.…”

Section: Introductionmentioning

confidence: 99%

Infection and Incidence of Herpes Viruses in Neonates in Aktobe Region of Kazakhstan

Zhumagaliyeva¹,

Mamyrbayeva²

2016

Biol Med (Aligarh)

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Section: Introductionmentioning

confidence: 99%

Infection and Incidence of Herpes Viruses in Neonates in Aktobe Region of Kazakhstan

Zhumagaliyeva¹,

Mamyrbayeva²

2016

Biol Med (Aligarh)

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“…Most likely, this rise in HCMV prevalence among parents of young children is via infection by their own children (Seale et al, 2006). Exposure to HCMV is increased by crowded living conditions, early sexual activity and greater numbers of sexual partners (Damato & Winnen, 2002).…”

Section: Hcmv Infection Of Immunocompromised Individualsmentioning

confidence: 99%

“…Clinical manifestations of cytomegalic inclusion disease at birth (microcephaly, hepatosplenomegaly, thrombocytopenic purpure, hearing loss, intracranial calcifications) are indicative of poor prognosis, culminating in death of 20 to 30% of neonates manifesting the disease (Damato & Winnen, 2002;Griffiths & Walter, 2005).…”

Section: Congenital Infectionmentioning

confidence: 99%

“…Thus, the diagnosis window of congenital infection is during the first three weeks of the neonate's life (Damato & Winnen, 2002;Griffiths & Walter, 2005;Stern-Ginossar et al, 2012).…”

Section: Congenital Infectionmentioning

confidence: 99%

“…Virus has been isolated from various body fluidsincluding saliva, blood, breast milk, urine, faeces, cervical secretions and semen -and, thereby, they are sources of infection. Infection occurs by viral entry through the mucous membrane and through the bloodstream, which allows numerous manners of contraction (Damato & Winnen, 2002;Seitz et al, 2010). Transmission may also be related to cultural factors .…”

Section: Hcmv Infection Of Immunocompromised Individualsmentioning

confidence: 99%

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Cell-type specific activities of cytomegalovirus GPCR homologues

Oliveira¹

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The human cytomegalovirus (HCMV) is the main agent of congenital infection worldwide. Although primary infections or reactivations do not represent a threat to most individuals, they impose a lifethreating event to a developing foetus or immunocompromised individuals. Although we have learnt much about sites of viral replication and cells involved in the end-stage disease, the specific pathway and essential cell types that the virus must go through to later establish a successful infection and latency is still not clear. HCMV encodes four GPCR homologues (vGPCR): US27, US28, UL33 and UL78. vGPCR have been implicated in viral pathogenesis due to their ability to activate signalling pathways and, thus, alter physiological processes to favour infection. US28 and UL33 have been shown to activate several kinases and transcriptional factors. A key component of both US28 and UL33 sequence is the DRY (Asp/Arg/Tyr) motif, a region directly involved with G protein binding.Because Gα protein content is cell type-dependent, signalling activation by vGPCR can differ from cell to cell. Furthermore, these receptors share the particular characteristic of being constitutively Functional studies demonstrated migration induction of HTR-8 following transfection by either US28 or UL33. In order to investigate potential mechanisms, signalling pathways were probed via detection of activated kinases and the secretion of matrix metalloproteinases (MMP) and chemokines (CK) via luminex assays. Although differences in the level of kinases could not be measured by western blot, induction of MMP and CK were detected by Luminex assays, with contrasting results for US28 and UL33 in transfected cells (HTR-8 and HUVEC). An HCMV recombinant disrupted for US28 signalling (DQY) was generated and compared with wild type HCMV and a US28 null mutant for induction of MMP and CK in virus-infected cells. US28-dependent effects were identified for infected human foreskin fibroblasts, but results for HUVEC were equivocal.iii To determine potential bottlenecks to viral dissemination that may require M33 function(s), mice were infected with M33 knockout (M33) viruses by different routes: intranasal, -mimicking the most natural route, footpad -a more compartmentalized route, and intraperitoneal, the most direct route to major organs. To help track viral dissemination, a luciferase tagged M33 was generated and infection was compared to control virus (M78luc). M33 intranasal infection did not result in attenuation for colonisation of the nose, draining (superficial cervical) lymph nodes (dLN) or the lungs. Via footpad route, M33 colonised the footpad and dLN to levels equivalent to control virus, but was significantly attenuated in spread to the spleen and, subsequently, the salivary glands.Following intraperitoneal infection, the virus is readily delivered to major organs due to direct access to the bloodstream, there was no difference in the colonisation of primary organs (spleen, liver) between M33 and control MCMV, but M33 was still unable ...

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Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

Liu

Líu

et al. 2021

Journal of Medical Virology

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Determination of human cytomegalovirus IgG (HCMV IgG) level is of great importance in the diagnosis of HCMV infections. In this study, a novel, double antigen sandwich homogeneous immunoassay‐based light‐initiated chemiluminescent assay (LICA) for measuring HCMV IgG serum levels was developed. This sandwich LICA for HCMV IgG was performed by incubating serum samples with HCMV pp150 protein coated with chemibeads, streptavidin‐coated sensibeads, and biotinylated HCMV pp150 protein. The working conditions of this assay were optimized and the correlation between the results of the LICA and enzyme‐linked immunosorbent assay was evaluated. As a homogeneous immunoassay, this sandwich LICA could accurately and rapidly determine the serum levels of HCMV IgG with a high‐throughput. Thus, this newly developed assay could be a useful analytical tool in the clinical diagnosis of HCMV infections.

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Cytomegalovirus Infection: Perinatal Implications

Cited by 40 publications

References 32 publications

Infection and Incidence of Herpes Viruses in Neonates in Aktobe Region of Kazakhstan

Infection and Incidence of Herpes Viruses in Neonates in Aktobe Region of Kazakhstan

Cell-type specific activities of cytomegalovirus GPCR homologues

Establishment of a sandwich light‐initiated chemiluminescence assay with double antigen for detecting human cytomegalovirus IgG antibody

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