Cytomegalovirus <scp>DNA</scp> is highly prevalent in the blood of patients with asthma and is associated with age and asthma traits

Kowalski, Marek L.; Wardzyńska, Aleksandra; Studzińska, Mirosława; Pawełczyk, Małgorzata; Leśnikowski, Zbigniew J.; Paradowska, Edyta

doi:10.1111/all.13233

Cited by 12 publications

(11 citation statements)

References 15 publications

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“…EVD68 has been associated with asthma exacerbation and pneumonia in children [ 20 ], In this study, EV-D68 was detected in the 2014–2015 winter seasons, confirming the presence of this virus in Mexico City. With respect to CMV, some studies have found evidence of its implications in asthma pathogenesis and asthmatic inflammation [ 27 ]. InfA H1N1pdm09 was found in most of the studied groups, its participation and association with bacterial pneumonia [ 7 ] are well known, but a strong association with asthma exacerbation has been reported as well [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Virome and bacteriome characterization of children with pneumonia and asthma in Mexico City during winter seasons 2014 and 2015

et al. 2018

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BackgroundAcute asthma exacerbations and pneumonia are important causes of morbidity and mortality in children and may coexist in the same children, although symptom overlap may lead to difficulties in diagnosis. Microbial and viral diversity and differential abundance of either may play an important role in infection susceptibility and the development of acute and chronic respiratory diseases.ObjectivesTo describe the virome and bacteriome present in the upper respiratory tract of hospitalized children with a clinical diagnosis of asthma and pneumonia during an acute exacerbation and an acute respiratory illness ARI episode respectively.MethodsDuring the winter seasons of 2013–2014 and 2014–2015, 134 nasopharyngeal swabs samples of children <15 years of age with ARI hospitalized at a referral hospital for respiratory diseases were selected based on clinical diagnosis of asthma or pneumonia. The virome and bacteriome were characterized using Whole Genome Sequencing (WGS) and in-house bioinformatics analysis pipeline.ResultsThe Asthma group was represented mainly by RV-C, BoV-1 and RSV-B and the pneumonia group by Bacteriophage EJ-1 and TTMV. TTV was found in both groups with a similar amount of reads. About bacterial composition Moraxella catarrhalis, Propionibacterium acnes and Acinetobacter were present in asthma and Veillonella parvula and Mycoplasma pneumoniae in pneumonia. Streptococcus pneumoniae and Haemophilus influenzae were mostly found with both asthma and pneumonia.ConclusionsOur results show a complex viral and bacterial composition in asthma and pneumonia groups with a strong association of RV-C presence in asthmatic children. We observed Streptococcus pneumoniae and Haemophilus influenzae concurrently in both groups.

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Section: Discussionmentioning

confidence: 99%

Virome and bacteriome characterization of children with pneumonia and asthma in Mexico City during winter seasons 2014 and 2015

et al. 2018

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“…The pathophysiology of recurrent wheeze in young children is unclear. Persistent bronchial inflammation was thought to play an essential role, but more recently it has been reported that the presence of CMV DNA in the blood of adult and elderly patients was associated with an increased risk of asthma, and that CMV DNA copy numbers correlated with certain asthma traits [ 8 ]. This suggests that CMV infection may play a role in the pathophysiology of recurrent wheeze.…”

Section: Introductionmentioning

confidence: 99%

Associations between patient clinical characteristics and the presence of cytomegalovirus DNA in the bronchoalveolar lavage fluid of children with recurrent wheezing

Sun

Wang

et al. 2018

BMC Infect Dis

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BackgroundThis study aimed to investigate the occurrence of Cytomegalovirus (CMV) DNA in the Bronchoalveolar lavage fluid (BALF) of children with recurrent wheezing and to identify associations with certain patient clinical characteristics.MethodsIn this cross-sectional study, pediatric patients (age < 36 months) admitted to Soochow University Hospital with recurrent wheezing (≥ 4 episodes of wheezing per year) were enrolled in the study. Cytomegalovirus DNA from their BALF was detected by real-time PCR. Subpopulations of blood immunoglobulins and T lymphocytes were quantified. The clinical characteristics of patients with and without BALF CMV DNA were compared. Comparisons of non-normally distributed continuous variables between groups were made using the Mann-Whitney U-test. Comparisons of frequency distributions were made using the Chi-squared test. Spearman’s rank correlation coefficient was used to evaluate correlations between the number of CMV DNA copies and continuous variables.ResultsA total of 111 patients aged 4 to 36 months (median 14.0 (IQR 8.0–22.0) months) were enrolled on to the study. Cytomegalovirus DNA was detected in 51.4% of patients (n = 111) with recurrent wheeze and was more prevalent among those aged 12 to 36 months with a positive modified asthma predictive index (mAPI) (n = 38, median 23.5 (IQR 19.7–31.2) months) than in those of the same age group with a negative mAPI (n = 25, median 15.0 (IQR 13.0–19.0) months) (57.9% vs. 20.0%, p = 0.003). Bronchoalveolar lavage fluid CMV DNA copy number [median 7560 (IQR 1200–71,150) copies/mL] was positively correlated with the duration of hospitalization (r = 0.33, p = 0.013), and negatively correlated with patient age (r = − 0.41, p = 0.002) and the percentage of BALF eosinophils (r = − 0.38, p = 0.004).ConclusionsCMV infection or reactivation in children with recurrent wheeze is associated with certain clinical characteristics, including younger age and lower levels of BALF eosinophils. Higher CMV DNA copy numbers were associated with a longer duration of hospitalization. Further studies are needed to address whether specific antiviral treatment could be beneficial for BALF CMV positive patients.

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“…2,3 A case-control study comparing CMV DNA between adults with and without asthma, demonstrated that those with asthma were more likely to be positive for CMV DNA. 25 Additionally, the number of CMV copies was higher in elderly, defined as age above 65 years, with asthma as compared to elderly without asthma. The number of CMV copies was not different in non-elderly with and without asthma.…”

Section: Comparison With Previous Studiesmentioning

confidence: 97%

The influence of Epstein‐Barr virus and cytomegalovirus on childhood respiratory health: A population‐based prospective cohort study

Meel

Jaddoe

Zelm

et al. 2020

Clin Experimental Allergy

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Background Epstein‐Barr virus (EBV) and cytomegalovirus (CMV) infection are common in early childhood. CMV infection favours a T‐helper‐1 and EBV infection a T‐helper‐2 cell response, possibly leading to disbalanced T‐helper cell response, and subsequent risk of asthma or atopy. Objective To study the associations of EBV and CMV with lung function, asthma and inhalant allergic sensitization at school age. Methods This study among 3546 children was embedded in a population‐based prospective cohort. At age 6 years, serum IgG levels against EBV and CMV were measured by ELISA. At age 10 years, lung function was measured by spirometry, asthma by questionnaire and inhalant allergic sensitization by skin prick test. Results Unadjusted models showed that seropositivity for EBV was associated with a higher FEV1 and FEF75 (Z‐score difference (95% CI): 0.09 (0.02, 0.16) and 0.09 (0.02, 0.15)), while seropositivity for CMV was not. Specific combinations of viruses showed that seropositivity for EBV was only associated with FEV1 and FEF75 in the presence of seropositivity for CMV (0.12 (0.04, 0.20)) and 0.08 (0.01, 0.15)). Seropositivity for CMV in the absence of seropositivity for EBV was associated with an increased risk of inhalant allergic sensitization (OR (95% CI): 1.31 (1.02, 1.68)). All effect estimates attenuated into non‐significant mainly after adjustment for child's ethnicity. Seropositivity for EBV or CMV was not associated with asthma. Conclusions and Clinical Relevance Associations of EBV and CMV infections in early childhood with school‐age lung function and inhalant allergic sensitization are explained by ethnicity, or sociodemographic and lifestyle‐related factors.

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Cytomegalovirus DNA is highly prevalent in the blood of patients with asthma and is associated with age and asthma traits

Cited by 12 publications

References 15 publications

Virome and bacteriome characterization of children with pneumonia and asthma in Mexico City during winter seasons 2014 and 2015

Virome and bacteriome characterization of children with pneumonia and asthma in Mexico City during winter seasons 2014 and 2015

Associations between patient clinical characteristics and the presence of cytomegalovirus DNA in the bronchoalveolar lavage fluid of children with recurrent wheezing

The influence of Epstein‐Barr virus and cytomegalovirus on childhood respiratory health: A population‐based prospective cohort study

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